LOCUS       40924_32648        5935 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMTL82254, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Minton NP.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   J. Microbiol. Methods 78 (1), 79-85 (2009)
  PUBMED    19445976
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Pennington OJ, Heap JT, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The 
            University of Nottingham, University Park, Nottingham NG7 2RD, 
            United Kingdom
REFERENCE   3  (bases 1 to 5935)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2009"; volume: "78"; issue: "1"; pages: 
            "79-85"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2009) Centre for Biomolecular Sciences, The University of 
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /note="terminator derived from CD0164 gene of Clostridium 
                     difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             98..718
                     /gene="catP"
                     /label=catP
                     /note="Chloramphenicol acetyltransferase from Clostridium 
                     perfringens. Accession#: P26826"
     primer_bind     complement(767..783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      931..972
                     /note="terminator derived from fdx gene of Clostridium 
                     pasteurianum"
                     /regulatory_class="terminator"
     CDS             1493..2548
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label=repA
                     /protein_id="ACR43890.1"
                     /translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNIE
                     RTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRKEE
                     NKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQKEKY
                     ITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRERWLE
                     LWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKALKGKQV
                     LVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTEDEKEEL
                     NQDLIDEIEID"
     gene            1493..2548
                     /gene="repA"
                     /label=repA
     CDS             complement(2639..3142)
                     /codon_start=1
                     /product="putative plasmid replication protein"
                     /label=putative plasmid replication protein
                     /note="orf2"
                     /protein_id="ACR43893.1"
                     /translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYVG
                     PEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFATVT
                     IKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFKKEL
                     KTKN"
     CDS             3488..4222
                     /gene="ermBP"
                     /label=ermBP
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      4379..4967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            5288..5397
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             5430..5798
                     /label=traJ
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg gtatttgaaa aaattgataa
      121 aaatagttgg aacagaaaag agtattttga ccactacttt gcaagtgtac cttgtaccta
      181 cagcatgacc gttaaagtgg atatcacaca aataaaggaa aagggaatga aactatatcc
      241 tgcaatgctt tattatattg caatgattgt aaaccgccat tcagagttta ggacggcaat
      301 caatcaagat ggtgaattgg ggatatatga tgagatgata ccaagctata caatatttca
      361 caatgatact gaaacatttt ccagcctttg gactgagtgt aagtctgact ttaaatcatt
      421 tttagcagat tatgaaagtg atacgcaacg gtatggaaac aatcatagaa tggaaggaaa
      481 gccaaatgct ccggaaaaca tttttaatgt atctatgata ccgtggtcaa ccttcgatgg
      541 ctttaatctg aatttgcaga aaggatatga ttatttgatt cctattttta ctatggggaa
      601 atattataaa gaagataaca aaattatact tcctttggca attcaagttc atcacgcagt
      661 atgtgacgga tttcacattt gccgttttgt aaacgaattg caggaattga taaatagtta
      721 aacgcgtcca tggagatctc gaggcctgca gacatgcaag cttggcactg gccgtcgttt
      781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      901 tgcgcagcct gaatggcgaa tggcgctagc ataaaaataa gaagcctgca tttgcaggct
      961 tcttattttt atggcgcgcc gttctgaatc cttagctaat ggttcaacag gtaactatga
     1021 cgaagatagc accctggata agtctgtaat ggattctaag gcatttaatg aagacgtgta
     1081 tataaaatgt gctaatgaaa aagaaaatgc gttaaaagag cctaaaatga gttcaaatgg
     1141 ttttgaaatt gattggtagt ttaatttaat atattttttc tattggctat ctcgatacct
     1201 atagaatctt ctgttcactt ttgtttttga aatataaaaa ggggcttttt agcccctttt
     1261 ttttaaaact ccggaggagt ttcttcattc ttgatactat acgtaactat tttcgatttg
     1321 acttcattgt caattaagct agtaaaatca atggttaaaa aacaaaaaac ttgcattttt
     1381 ctacctagta atttataatt ttaagtgtcg agtttaaaag tataatttac caggaaagga
     1441 gcaagttttt taataaggaa aaatttttcc ttttaaaatt ctatttcgtt atatgactaa
     1501 ttataatcaa aaaaatgaaa ataaacaaga ggtaaaaact gctttagaga aatgtactga
     1561 taaaaaaaga aaaaatccta gatttacgtc atacatagca cctttaacta ctaagaaaaa
     1621 tattgaaagg acttccactt gtggagatta tttgtttatg ttgagtgatg cagacttaga
     1681 acattttaaa ttacataaag gtaatttttg cggtaataga ttttgtccaa tgtgtagttg
     1741 gcgacttgct tgtaaggata gtttagaaat atctattctt atggagcatt taagaaaaga
     1801 agaaaataaa gagtttatat ttttaactct tacaactcca aatgtaaaaa gttatgatct
     1861 taattattct attaaacaat ataataaatc ttttaaaaaa ttaatggagc gtaaggaagt
     1921 taaggatata actaaaggtt atataagaaa attagaagta acttaccaaa aggaaaaata
     1981 cataacaaag gatttatgga aaataaaaaa agattattat caaaaaaaag gacttgaaat
     2041 tggtgattta gaacctaatt ttgatactta taatcctcat tttcatgtag ttattgcagt
     2101 taataaaagt tattttacag ataaaaatta ttatataaat cgagaaagat ggttggaatt
     2161 atggaagttt gctactaagg atgattctat aactcaagtt gatgttagaa aagcaaaaat
     2221 taatgattat aaagaggttt acgaacttgc gaaatattca gctaaagaca ctgattattt
     2281 aatatcgagg ccagtatttg aaatttttta taaagcatta aaaggcaagc aggtattagt
     2341 ttttagtgga ttttttaaag atgcacacaa attgtacaag caaggaaaac ttgatgttta
     2401 taaaaagaaa gatgaaatta aatatgtcta tatagtttat tataattggt gcaaaaaaca
     2461 atatgaaaaa actagaataa gggaacttac ggaagatgaa aaagaagaat taaatcaaga
     2521 tttaatagat gaaatagaaa tagattaaag tgtaactata ctttatatat atatgattaa
     2581 aaaaataaaa aacaacagcc tattaggttg ttgtttttta ttttctttat taattttttt
     2641 aatttttagt ttttagttct tttttaaaat aagtttcagc ctctttttca atatttttta
     2701 aagaaggagt atttgcatga attgcctttt ttctaacaga cttaggaaat attttaacag
     2761 tatcttcttg cgccggtgat tttggaactt cataacttac taatttataa ttattatttt
     2821 cttttttaat tgtaacagtt gcaaaagaag ctgaacctgt tccttcaact agtttatcat
     2881 cttcaatata atattcttga cctatatagt ataaatatat ttttattata tttttacttt
     2941 tttctgaatc tattatttta taatcataaa aagttttacc accaaaagaa ggttgtactc
     3001 cttctggtcc aacatatttt tttactatat tatctaaata atttttggga actggtgttg
     3061 taatttgatt aatcgaacaa ccagttatac ttaaaggaat tataactata aaaatatata
     3121 ggattatctt tttaaatttc attattggcc tcctttttat taaatttatg ttaccataaa
     3181 aaggacataa cgggaatatg tagaatattt ttaatgtaga caaaatttta cataaatata
     3241 aagaaaggaa gtgtttgttt aaattttata gcaaactatc aaaaattagg gggataaaaa
     3301 tttatgaaaa aaaggttttc gatgttattt ttatgtttaa ctttaatagt ttgtggttta
     3361 tttacaaatt cggccggccg aagcaaactt aagagtgtgt tgatagtgca gtatcttaaa
     3421 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
     3481 tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact
     3541 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
     3601 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
     3661 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
     3721 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
     3781 tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca
     3841 tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca
     3901 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
     3961 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca
     4021 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt
     4081 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc
     4141 caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta
     4201 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac
     4261 acgttactaa agggaatgtg tttaaactcc tttttgataa tctcatgacc aaaatccctt
     4321 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4501 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4561 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4621 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4681 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4741 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4801 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4861 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4921 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4981 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5041 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5101 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5161 gcagggcccc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     5221 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     5281 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     5341 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     5401 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     5461 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg
     5521 cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa
     5581 tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg
     5641 gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg
     5701 gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca
     5761 aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac
     5821 ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagagcgac
     5881 ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgatcg ggccc
//
