LOCUS Exported 3416 bp ds-DNA circular SYN 03-12-2013 DEFINITION Bacterial expression vector with a T5 promoter. For other reading frames, use pQE-4 or pQE-5. ACCESSION . VERSION . KEYWORDS pQE-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3416) AUTHORS Qiagen TITLE Direct Submission COMMENT Discontinued pQE vector. FEATURES Location/Qualifiers source 1..3416 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 10..54 /note="T5 promoter" /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 30..46 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 62..78 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" CDS 115..117 /codon_start=1 /product="start codon" /note="ATG" /translation="M" misc_feature 121..146 /note="MCS" /note="multiple cloning site" misc_feature 146..156 /note="stop codons" /note="stop codons in all three reading frames" terminator 162..256 /gene=" " /note="lambda t0 terminator" /note="transcription terminator from phage lambda" CDS 300..959 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" terminator 1024..1110 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin complement(1592..2180) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2351..3211) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS LIKHW" promoter complement(3212..3316) /gene="bla" /note="AmpR promoter" ORIGIN 1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 121 ggatccgtcg acctgcagcc aagcttaatt agctgagctt ggactcctgt tgatagatcc 181 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt 241 ttttattggt gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa 301 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 361 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 421 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 481 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg 541 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 601 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 661 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 721 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 781 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 841 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg 901 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat 961 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg 1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1081 tcggtgaacg ctctcctgag taggacaaat ccgccgctct agagctgcct cgcgcgtttc 1141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 1201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 1261 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg 1321 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 1381 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc 1441 gctcggtctg tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 2221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 2281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 2341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 2401 gttcatccat agctgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 2461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 2521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 2581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 2641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 2701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 2761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 2821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 2881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 2941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 3001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 3301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 3361 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac //