LOCUS 40924_39473 3864 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pSEVA343, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3864) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3864) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3864) TITLE Direct Submission REFERENCE 4 (bases 1 to 3864) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3864 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 125..146 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 161..191 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 199..215 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 223..239 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 249..305 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(309..325) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(329..352) /label=F24 /note="F24" terminator 604..698 /label=lambda t0 terminator /note="transcription terminator from phage lambda" promoter 746..848 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 849..1505 /codon_start=1 /label=CmR /note="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" oriT 1657..1765 /label=oriT /note="incP origin of transfer" rep_origin 1859..2447 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2504..3334) /codon_start=1 /label=pRO1600 Rep /note="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa" /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA" rep_origin 3348..3699 /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" terminator complement(3772..3858) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca 301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat 481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc 601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa 661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg 721 atttaaattg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata 781 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg 841 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc 901 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc 961 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg 1021 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga 1081 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc 1141 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac 1201 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt 1261 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt 1321 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata 1381 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg 1441 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg 1501 gggcgtaatt tgacttttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc 1561 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg 1621 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc 1681 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg 1741 gaatcctgct ctgcgaggct ggccgtaggc cggccgataa tctcatgacc aaaatccctt 1801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 2041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2281 gaaaggcgga caggcatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2461 cggccgtgaa aggcaggccg gtccgtggtg gccacggcct ctaggccaga tccagcggca 2521 tctgggttag tcgagcgcgg gccgcttccc atgtctcacc agggcgagcc tgtttcgcga 2581 tctcagcatc tgaaatcttc ccggccttgc gcttcgctgg ggccttaccc accgccttgg 2641 cgggcttctt cggtccaaaa ctgaacaaca gatgtgtgac cttgcgcccg gtctttcgct 2701 gcgcccactc cacctgtagc gggctgtgct cgttgatctg cgtcacggct ggatcaagca 2761 ctcgcaactt gaagtccttg atcgagggat accggccttc cagttgaaac cactttcgca 2821 gctggtcaat ttctatttcg cgctggccga tgctgtccca ttgcatgagc agctcgtaaa 2881 gcctgatcgc gtgggtgctg tccatcttgg ccacgtcagc caaggcgtat ttggtgaact 2941 gtttggtgag ttccgtcagg tacggcagca tgtctttggt gaacctgagt tctacacggc 3001 cctcaccctc ccggtagatg attgtttgca cccagccggt aatcatcaca ctcggtcttt 3061 tccccttgcc attgggctct tgggttaacc ggacttcccg ccgtttcagg cgcagggccg 3121 cttctttgag ctggttgtag gaagattcga tagggacacc cgccatcgtc gctatgtcct 3181 ccgccgtcac tgaatacatc acttcatcgg tgacaggctc gctcctcttc acctggctaa 3241 tacaggccag aacgatccgc tgttcctgaa cactgaggcg atacgcggcc tcgaccaggg 3301 cattgctttt gtaaaccatt gggggtgagg ccacgttcga cattccttgt gtataagggg 3361 acactgtatc tgcgtcccac aatacaacaa atccgtccct ttacaacaac aaatccgtcc 3421 cttcttaaca acaaatccgt cccttaatgg caacaaatcc gtcccttttt aaactctaca 3481 ggccacggat tacgtggcct gtagacgtcc taaaaggttt aaaagggaaa aggaagaaaa 3541 gggtggaaac gcaaaaaacg caccactacg tggccccgtt ggggccgcat ttgtgcccct 3601 gaaggggcgg gggaggcgtc tgggcaatcc ccgttttacc agtcccctat cgccgcctga 3661 gagggcgcag gaagcgagta atcagggtat cgaggcggat tcacccttgg cgtccaacca 3721 gcggcaccag cggcgcctga gaggggcgcg cccagctgtc tagggcggcg gatttgtcct 3781 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg 3841 agcctttcgt tttatttgat gcct //