LOCUS       40924_45213        6388 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.GATA4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 6388)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1817..3136
                     /codon_start=1
                     /gene="GATA4"
                     /product="Homo sapiens GATA4"
                     /label=GATA4
                     /protein_id="ABM67539.1"
                     /translation="MYQSLPWPPTTGRPPVPTRRAAPAPSCTARARPRQSTCPHRGALL
                     RAGPVLPPGRRRGLCVRRPSGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSP
                     RFSFPGTTGSLAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAAFAGSYSSPYPAYM
                     ADVGASWAAAAAASAGPFDSPVLHSLPGRANPGARHPNLDMFDDFSEGRECVNCGAMST
                     PLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRR
                     NAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPA
                     SGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSA
                     LKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA"
     gene            1817..3136
                     /gene="GATA4"
                     /label=GATA4
     polyA_signal    3357..3412
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3789..3805)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4279..4383
                     /label=AmpR promoter
     CDS             4384..5241
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5415..6003
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6291..6312
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6327..6357
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6365..6381
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctcgca gggaccatgt atcagagctt gccatggccg ccaaccacgg gccgcccccc
     1861 ggtgcctacg aggcgggcgg ccccggcgcc ttcatgcacg gcgcgggcgc gtcctcgcca
     1921 gtctacgtgc ccacaccgcg gtgccctcct ccgtgctggg cctgtcctac ctccagggcg
     1981 gaggcgcggg ctctgcgtcc ggaggccctc gggcggcagc tccggtgggg ccgcgtctgg
     2041 tgcggggccc gggacccagc agggcagccc gggatggagc caggcgggag ccgacggagc
     2101 cgcttacacc ccgccgccgg tgtcgccgcg cttctccttc ccggggacca ccgggtccct
     2161 ggcggccgcc gccgctgccg cggcccggga agctgcggcc tacagcagtg gcggcggagc
     2221 ggcgggtgcg ggcctggcgg gccgcgagca gtacgggcgc gccgcgttcg cgggctccta
     2281 ctccagcccc tacccggctt acatggccga cgtgggcgcg tcctgggccg cagccgccgc
     2341 cgcctccgcc ggccccttcg acagcccggt cctgcacagc ctgcccggcc gggccaaccc
     2401 cggcgcccga caccccaatc tcgatatgtt tgacgacttc tcagaaggca gagagtgtgt
     2461 caactgtggg gctatgtcca ccccgctctg gaggcgagat gggacgggtc actatctgtg
     2521 caacgcctgc ggcctctacc acaagatgaa cggcatcaac cggccgctca tcaagcctca
     2581 gcgccggctg tccgcctccc gccgagtggg cctctcctgt gccaactgcc agaccaccac
     2641 caccacgctg tggcgccgca atgcggaggg cgagcctgtg tgcaatgcct gcggcctcta
     2701 catgaagctc cacggggtcc ccaggcctct tgcaatgcgg aaagagggga tccaaaccag
     2761 aaaacggaag cccaagaacc tgaataaatc taagacacca gcagctcctt caggcagtga
     2821 gagccttcct cccgccagcg gtgcttccag caactccagc aacgccacca ccagcagcag
     2881 cgaggagatg cgtcccatca agacggagcc tggcctgtca tctcactacg ggcacagcag
     2941 ctccgtgtcc cagacgttct cagtcagtgc gatgtctggc catgggccct ccatccaccc
     3001 tgtcctctcg gccctgaagc tctccccaca aggctatgcg tctcccgtca gccagtctcc
     3061 acagaccagc tccaagcagg actcttggaa cagcctggtc ttggccgaca gtcacgggga
     3121 cataatcact gcgtaatctt ccctcttccc tcatcccatg gaagctgacg ggcccggtac
     3181 cgcggccgca cttaagttac gcgtggatca attcactcct caggtgcagg ctgcctatca
     3241 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3301 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3361 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3421 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3481 aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata
     3541 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     3601 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     3661 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     3721 ttctcttatg aagatccctc gacctgcagc ccaagctgat cccgggattt aaatgtttaa
     3781 acgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     3961 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     4021 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4081 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4141 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagcccg tgagcgtggg
     5101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5521 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6241 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6301 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6361 ggaattgtga gcggataaca atttcaca
//
