LOCUS       pYES2_NT_B.        6039 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector for galactose-inducible expression of 
            N-terminally tagged proteins in S. cerevisiae. For other reading 
            frames, use pYES2/NT A or pYES2/NT C.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2 NT B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             510..512
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             522..539
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             543..575
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             579..602
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    602..670
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             683..724
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             734..751
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      785..1032
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1280..1868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2042..2899)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2998..3798)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3799..4019)
                     /label=URA3 promoter
     rep_origin      complement(4618..5498)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5509..6022)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttacca tggggggttc tcatcatcat catcatcatg
      541 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
      601 aggtacctaa ggatccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct
      661 cgagtctaga gggcccttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
      721 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cctagagggc
      781 cgcatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
      841 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
      901 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
      961 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     1021 gctttaattt gcaagctgcg gccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaagcccagg aaccgtaaaa
     1261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2041 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2101 ttgcctgact ccccgtcgtg tagataacta cgatacggga gcgcttacca tctggcccca
     2161 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2221 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2281 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2341 ttgttggcat tgctacaggc atcgtggtgt cactctcgtc gtttggtatg gcttcattca
     2401 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2461 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2521 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2581 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2641 cttgcccggc gtcaatacgg gataatagtg tatcacatag cagaacttta aaagtgctca
     2701 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2761 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2821 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2881 ggaaatgttg aatactcata ctcttccttt ttcaatgggt aataactgat ataattaaat
     2941 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     3001 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     3061 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     3121 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     3181 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     3241 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     3301 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     3361 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     3421 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     3481 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     3541 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     3601 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     3661 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     3721 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     3781 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     3841 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     3901 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     3961 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
     4021 agctagctta tcgatgataa gctgtcaaag atgagaatta attccacgga ctatagacta
     4081 tactagatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     4141 gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg tgatctaaga
     4201 ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga aatgcaaaag
     4261 gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct tctcaatgat
     4321 attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac
     4381 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     4441 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     4501 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     4561 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     4621 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     4681 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     4741 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgacg agagcgctaa
     4801 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     4861 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     4921 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     4981 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     5041 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     5101 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     5161 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     5221 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     5281 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     5341 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     5401 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     5461 tacttttgag caatgtttgt ggaagcggta ttcgcaatgg gaagctccac cccggttgat
     5521 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaaacgtt
     5581 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taacgaatag
     5641 cccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5701 gttccagttt ccaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5761 aaaagggtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5821 gggtcgaggt gccgtaaagc agtaaatcgg aagggtaaac ggatgccccc atttagagct
     5881 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggg
     5941 gctagggcgg tgggaagtgt aggggtcacg ctgggcgtaa ccaccacacc cgccgcgctt
     6001 aatggggcgc tacagggcgc gtggggatga tccactagt
//
