LOCUS       V001890                 7420 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001890
VERSION     V001890
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     primer_bind     685..701
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        708..726
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     759..775
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(780..1496)
                     /label="EYFP"
                     /note="enhanced YFP"
     misc_feature    complement(1527..1562)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1721..2368
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2736..3772
                     /label="amyE back"
                     /note="amyE back"
     CDS             4972..5160
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5265..5405
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5591..6179)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6353..7210)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7211..7315)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgaaata gtacataatg ggaattcgaa
      661 ttcggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
      721 tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt ggatctttac
      781 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      841 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg
      901 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      961 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
     1021 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
     1081 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     1141 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     1201 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     1261 atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc cgaaggtggt cacgagggtg
     1321 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     1381 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
     1441 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
     1501 gcaaattcgc ttaccttttc tattgaatcc atagtagttc ctccttcccg ggaaagcccg
     1561 cggtaaaaga attcgaaaat tggataaagt gggatatttt taaaatatat atttatgtta
     1621 cagtaatatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac aagtaagcct
     1681 cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta ataaaattga
     1741 tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac aaacgacttt
     1801 tagtataacc acagaaattg atattagtgt tttataccga aacataaaac aagaaggata
     1861 taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa atacagcttt
     1921 tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag agccacttta
     1981 tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg taaagaatga
     2041 cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg gttcggggaa
     2101 attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta ttccatggac
     2161 ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc tacccattat
     2221 tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat ctttacaggt
     2281 acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta ttcaggaatt
     2341 gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg tactttttac
     2401 agtcggtttt ctaatgtcac taacctgccc cgttagttga agaaggtttt tatattacag
     2461 ctccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     2521 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     2581 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     2641 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     2701 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgacatgg atgagcgatg
     2761 atgatatccg tttaggctgg gcggtgatag cttctcgttc aggcagtacg cctcttttct
     2821 tttccagacc tgagggaggc ggaaatggtg tgaggttccc cagatctggg ggaaaagcca
     2881 aataggcgat cgcgggagtg ctttatttga agatcaggct atcactgcgg tcaatagatt
     2941 tcacaatgtg atggctggac agcctgagga actctcgaac ccgaatggaa acaaccagat
     3001 atttatgaat cagcgcggct cacatggcgt tgtgctggca aatgcaggtt catcctctgt
     3061 ctctatcaat acggcaacaa aattgcctga tggcaggtat gacaataaag ctggagcggg
     3121 ttcatttcaa gtgaacgatg gtaaactgac aggcacgatc aatgccaggt ctgtagctgt
     3181 gctttatcct gatgatattg caaaagcgcc tcatgttttc cttgagaatt acaaaacagg
     3241 tgtaacacat tctttcaatg atcaactgac gattaccttg cgtgcagatg cgaatacaac
     3301 aaaagccgtt tatcaaatca ataatggacc agacgacagg cgtttaagga tggagatcaa
     3361 ttcacaatcg gaaaaggaga tccaatttgg caaaacatac accatcatgt taaaaggaac
     3421 gaacagtgat ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc
     3481 ggccaaaacc atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta
     3541 taaacatgat gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac
     3601 taaaaatgca gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc
     3661 aaaagtgatt tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga
     3721 ttacgtgcta aatggtttat ataatgactc gggcttaagc ggttctcttc cccattgagg
     3781 gcaaggctag acgggactta ccgaaagaaa ccatcaatga tggtttcttt tttgttcata
     3841 aatcagacaa aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata taaatgtgaa
     3901 atacttcaca aacaaaaaga catcaaagag aaacataccc tgcaaggatg ctgatattgt
     3961 ctgcatttgc gccggagcaa accaaaaacc tggtgagaca cgccttgaat ttgacggcat
     4021 tttcttagtc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     4081 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     4141 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct
     4201 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     4261 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     4321 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     4381 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     4441 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc
     4501 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
     4561 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     4621 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     4681 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     4741 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     4801 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     4861 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     4921 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     4981 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     5041 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     5101 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5161 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5221 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     5281 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     5341 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     5401 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5461 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5521 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5581 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5641 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5701 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5761 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5821 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5881 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5941 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6001 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6061 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6121 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6181 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6241 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6301 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6361 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6421 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6481 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6541 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6601 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6661 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6721 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6781 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6841 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6901 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6961 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7021 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7081 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7141 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7201 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7261 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7321 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7381 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
//
