LOCUS       V047330                 8519 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047330
VERSION     V047330
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1157..4060
                     /label="AARS1(NM_001605)"
                     /note="AARS1(NM_001605)"
                     /gene="AARS1"
     CDS             4061..4084
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    4119..4343
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4389..4817
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4831..5160
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5227..6021
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6195..6316
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6365..6381)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6389..6405
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6413..6443)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6458..6479
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6767..7352)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7523..8383)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8384..8488)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gcagcgaaat tggcacaggc ttcccgttcg acccgcatta
      961 cgttgaggtg ctgggcgagc gcatgcacta tgtggacgtg ggtccgcgtg acggtacccc
     1021 tgtgctgttc ctgcatggta acccgaccag cagctacgtg tggcgcaaca ttatcccgca
     1081 cgtggccccg ggatccggcg gttctggagg ctccggcggt tcagggggat cgggcggctc
     1141 aggcggctct gaattcatgg actctactct aacagcaagt gaaatccggc agcgatttat
     1201 agatttcttc aagaggaacg agcatacgta tgttcactcg tctgccacca tcccattgga
     1261 tgaccccact ttgctctttg ccaatgcagg catgaaccag tttaaaccca ttttcctgaa
     1321 cacaattgac ccatctcacc ccatggcaaa gctgagcaga gctgccaata cccagaagtg
     1381 catccgggct gggggcaaac ataatgacct ggacgatgtg ggcaaggatg tctatcatca
     1441 caccttcttc gagatgctgg gctcttggtc ttttggagat tactttaagg aattggcatg
     1501 taagatggct ctggaactcc tcacccaaga gtttggcatt cccattgaaa gactttatgt
     1561 tacttacttt ggcggggatg aagcagctgg cttagaagca gatctggaat gcaaacagat
     1621 ctggcaaaat ttggggctgg atgacaccaa aatcctccca ggcaacatga aggataactt
     1681 ctgggagatg ggtgacacgg gcccctgtgg tccttgcagt gagatccact acgaccggat
     1741 tggtggtcgg gacgccgcac atcttgtcaa ccaggacgac cctaatgtgc tggagatctg
     1801 gaaccttgtg ttcatccagt ataacaggga agctgatggc attctgaaac ctcttcccaa
     1861 gaaaagcatt gacacaggga tgggcctgga acgactggta tctgtgctgc agaataagat
     1921 gtccaactat gacactgacc tttttgtccc ttactttgaa gccattcaga agggcacagg
     1981 tgcccgacca tacactggga aagttggtgc tgaggatgcc gatgggattg acatggccta
     2041 ccgggtgctg gctgaccacg ctcggaccat cactgtggca ctggctgatg gtggccggcc
     2101 tgacaacaca gggcgtggat atgtgttgag acggattctc cgccgagctg tccgatacgc
     2161 ccatgaaaag ctcaatgcca gcaggggctt ctttgctacg ttagtggatg ttgtcgtcca
     2221 gtccctggga gatgcatttc ctgagctgaa gaaggaccca gacatggtga aggacatcat
     2281 taatgaagaa gaggtgcagt ttctcaagac tctcagcaga gggcgtcgca tcctggacag
     2341 gaaaattcag agcctgggag acagcaagac cattcccgga gacactgctt ggctcctcta
     2401 tgacacctat gggtttccag tggatctgac tggactgatt gctgaagaga agggcctggt
     2461 ggtagacatg gatggctttg aagaggagag gaaactggcc cagctgaaat cacagggcaa
     2521 gggagctggt ggggaagacc tcattatgct ggacatttac gctatcgaag agctccgggc
     2581 acggggtctg gaggtcacag atgattcccc aaagtacaat taccatttgg actccagtgg
     2641 tagctatgta tttgagaaca cagtggctac ggtgatggct ctgcgcaggg agaagatgtt
     2701 cgtggaagag gtgtccacag gccaggagtg tggagtggtg ctggacaaga cctgtttcta
     2761 tgctgagcaa ggaggccaga tctatgacga aggctacctg gtgaaggtgg atgacagcag
     2821 tgaagataaa acagagttta cagtgaagaa tgctcaggtc cgaggagggt atgtgctaca
     2881 cattggaacc atctacggtg acctgaaagt gggggatcag gtctggctgt ttattgatga
     2941 gccccgacga agacccatca tgagcaacca cacagctacg cacattctga acttcgccct
     3001 gcgctcagtg cttggggaag ctgaccagaa aggctcattg gttgctcctg accgcctcag
     3061 atttgacttt actgccaagg gagccatgtc cacccaacag atcaagaagg ctgaagagat
     3121 tgctaatgag atgattgagg cagccaaggc cgtctatacc caggattgcc ccctggcagc
     3181 agcgaaagcc atccagggcc tacgggctgt gtttgatgag acctatcctg accctgtgcg
     3241 agtcgtctcc attggggtcc cggtgtccga gttgctggat gacccctctg ggcctgctgg
     3301 ctccctgact tctgttgagt tctgtggggg aacgcacctg cggaactcga gtcatgcagg
     3361 agcttttgtg atcgtgacgg aagaagccat tgccaagggt atccggagga ttgtggctgt
     3421 cacaggtgcc gaggcccaga aggccctcag gaaagcagag agcttgaaga aatgtctctc
     3481 tgtcatggaa gccaaagtga aggctcagac tgctccaaac aaggatgtgc agagggagat
     3541 cgctgacctt ggagaggccc tggccactgc agtcatcccc cagtggcaga aggatgaatt
     3601 gcgggagact ctcaaatccc taaagaaggt catggatgac ttggaccgag ccagcaaagc
     3661 cgatgtccag aaacgagtgt tagagaagac gaagcagttc atcgacagca accccaacca
     3721 gcctcttgtc atcctggaga tggagagcgg cgcctcagcc aaggccctga atgaagcctt
     3781 gaagctcttc aagatgcact cccctcagac ttctgccatg ctcttcacgg tggacaatga
     3841 ggctggcaag atcacgtgcc tgtgtcaagt tccccagaat gcagccaatc ggggcttaaa
     3901 agccagcgag tgggtgcagc aggtgtcagg cttgatggac ggtaaaggtg gtggcaagga
     3961 tgtgtctgca caggccacag gcaagaacgt tggctgcctg caggaggcgc tgcagctggc
     4021 cacttccttc gcccagctgc gcctcgggga tgtaaagaac gattacaagg atgacgacga
     4081 taagtgaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4141 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4201 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4261 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4321 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4381 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4441 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4501 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4561 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4621 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4801 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     5101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5161 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     5221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5401 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5701 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5881 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6181 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6241 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6301 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6421 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     7321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7441 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7501 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7561 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7621 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7681 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7741 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7801 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7861 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7921 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7981 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8041 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8101 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8161 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8221 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8281 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8341 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8401 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8461 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
