LOCUS       V034302                 8394 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034302
VERSION     V034302
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..3909
                     /label="AARS2(NM_020745)"
                     /note="AARS2(NM_020745)"
                     /gene="AARS2"
     CDS             3931..3960
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3989..4213
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4259..4687
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4701..5030
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5097..5891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6067..6188
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6237..6253)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6261..6277
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6285..6315)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6330..6351
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6639..7227)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7398..8258)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8259..8363)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggca
      961 gcgtcagtgg cagctgcagc ccggaggctg cggcgggcca ttcgaaggtc gcccgcatgg
     1021 cggggcctca gccatcggcc gctctcatcg gagccccctg cagccaaggc ctcggccgtg
     1081 agggccgcct ttctgaactt ctttcgggac cgccatggcc accggctggt gccctccgct
     1141 tccgtgcggc cccgcggcga ccccagtttg ctttttgtca atgcgggcat gaaccagttc
     1201 aagccaatct ttctgggcac cgtggatcca cgaagcgaga tggcaggctt ccgacgtgtg
     1261 gccaacagcc agaaatgtgt gagagctgga ggacaccata acgacctgga agatgtgggt
     1321 cgagaccttt cccatcatac cttctttgaa atgcttggca attgggcctt tgggggtgaa
     1381 tattttaagg aggaggcttg taacatggcc tgggaactgc tgactcaggt ctatgggatc
     1441 cctgaggaaa ggctctggat ctcctacttt gatggtgacc ccaaggcagg gctggaccca
     1501 gacctggaga ccagggacat ctggctgagc ttaggggtgc ctgctagccg tgtgctttcc
     1561 tttggaccac aagagaactt ctgggagatg ggggatactg gcccttgtgg gccctgtact
     1621 gagatccact acgaccttgc tggtggggtg ggagcccccc agctggtaga gctttggaac
     1681 ctggtcttca tgcaacacaa cagagaggca gatggaagcc tgcagcccct gccccagcgg
     1741 catgtggaca caggaatggg cctggaaagg ctggtggctg tgctgcaagg caaacactcc
     1801 acctatgaca ctgacctctt ttccccgctg ctcaacgcca tacagcaggg ctgcagggca
     1861 cccccttact tgggccgagt aggggtggca gacgaggggc gcacagacac agcgtaccgc
     1921 gtggtggctg accacatccg cacactcagt gtctgcatct ctgatggcat cttccctggg
     1981 atgtcaggtc ccccgctggt tcttcgtcgg atcctgcgtc gagctgtgcg tttctccatg
     2041 gagatcttaa aggcaccacc tggcttccta ggcagcctgg tacctgtagt ggtggagaca
     2101 ctgggagatg cttatccaga actgcaaagg aactcagccc agatcgccaa cctggtgtca
     2161 gaggacgagg cagccttcct ggcctccctg gagcggggta ggcggatcat tgatcggact
     2221 ctgaggaccc tggggccttc agatatgttc cctgctgaag tggcctggtc cttgtcactg
     2281 tgtggagacc tgggactccc cttggacatg gtagagctga tgctggagga gaaaggggtc
     2341 cagctagact ccgctggact ggagcggttg gcccaagagg aggcccagca ccgggcacgg
     2401 caggctgagc cagttcagaa gcagggattg tggcttgatg tccatgcgct tggggagctg
     2461 cagcgccaag gagtgccccc aactgacgac agccccaagt acaactactc cctgcgaccc
     2521 agcggaagtt atgagttcgg cacctgtgag gcccaggtgt tgcaactgta tacagaggac
     2581 gggacagcag tggcctccgt ggggaaaggc cagcgctgtg gcctcctctt ggacaggacc
     2641 aacttctacg cagaacaggg gggccaggct tcagaccgtg gctacctggt gcgggcaggg
     2701 caagaggacg tgctgttccc agtagcccgg gcccaggtct gtggaggttt catcctgcat
     2761 gaggcagtag cccctgagtg cctgcggtta ggggaccagg tgcagctgca tgtggatgag
     2821 gcctggcgtc taggctgcat ggcgaagcat acggccaccc acctgctgaa ctgggcactg
     2881 aggcagaccc tgggccctgg cacagagcag cagggctccc atctcaatcc tgagcagctg
     2941 cgcttggatg tgaccaccca gaccccattg accccagagc agctccgggc agtggagaac
     3001 actgtgcagg aggccgtggg gcaggatgag gctgtgtaca tggaggaggt gcccctggcg
     3061 ctcactgccc aggtccctgg cctgcgctct ctggatgagg tttacccaga ccctgtgcgg
     3121 gtggtatcag tgggggtgcc cgtggcccat gcattggacc cagcctccca agccgcactg
     3181 cagacctctg tggagctatg ctgtgggacg cacctgttac gtactggggc tgtaggggac
     3241 ctggttatca tcggggaccg ccagctttcc aagggcacta cccgcctgct ggccgtcact
     3301 ggggagcagg cccagcaggc ccgagagcta ggccagagcc tggcccagga agtgaaagcg
     3361 gccactgagc ggctgagtct ggggagccgg gatgtggcgg aggcactgag gctgtccaag
     3421 gacataggac gactcattga agctgtggaa actgctgtga tgccccagtg gcagcggcgg
     3481 gagctgctgg ccacagtgaa gatgctgcag cggcgtgcca acactgccat ccgtaagctg
     3541 caaatgggac aggctgcaaa gaaaactcag gagctgctgg agcggcactc gaaggggcct
     3601 ctgattgtgg acacagtctc tgctgagtct ctctcagtgc tggtgaaggt ggtacggcag
     3661 ctgtgtgagc aggcccccag cacgtctgtg ctcctactca gcccccagcc catggggaag
     3721 gtgctgtgtg cctgtcaggt ggcccagggt gccatgccca ccttcacagc agaggcctgg
     3781 gcactggcag tgtgcagcca catggggggc aaggcgtggg gctcacgagt ggtggcccaa
     3841 ggcaccggaa gcactactga cctggaagct gccctcagta tagcccaaac ctatgccctc
     3901 agccagctcc tcgagtctag agggcccttc gaacaaaaac tcatctcaga agaggatctg
     3961 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4021 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4081 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4141 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4201 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4321 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4441 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4561 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4621 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4681 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4741 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4801 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4861 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4921 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4981 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5041 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5101 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5161 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5221 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5281 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5341 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5401 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5461 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5521 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5581 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5641 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5701 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5761 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5821 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5881 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg
     5941 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6001 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6061 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6181 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
