LOCUS       V039759                10313 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039759
VERSION     V039759
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..5833
                     /label="ABCA5(NM_018672)"
                     /note="ABCA5(NM_018672)"
                     /gene="ABCA5"
     polyA_signal    5880..6104
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6150..6578
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6592..6921
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6988..7782
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7956..8077
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8126..8142)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8150..8166
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8174..8204)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8219..8240
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8528..9113)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9284..10144)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10145..10249)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tccactgcaa ttagggaggt aggagtttgg agacagacca gaacacttct
      961 actgaagaat tacttaatta aatgcagaac caaaaagagt agtgttcagg aaattctttt
     1021 tccactattt tttttatttt ggttaatatt aattagcatg atgcatccaa ataagaaata
     1081 tgaagaagtg cctaatatag aactcaatcc tatggacaag tttactcttt ctaatctaat
     1141 tcttggatat actccagtga ctaatattac aagcagcatc atgcagaaag tgtctactga
     1201 tcatctacct gatgtcataa ttactgaaga atatacaaat gaaaaagaaa tgttaacatc
     1261 cagtctctct aagccgagca actttgtagg tgtggttttc aaagactcca tgtcctatga
     1321 acttcgtttt tttcctgata tgattccagt atcttctatt tatatggatt caagagctgg
     1381 ctgttcaaaa tcatgtgagg ctgctcagta ctggtcctca ggtttcacag ttttacaagc
     1441 atccatagat gctgccatta tacagttgaa gaccaatgtt tctctttgga aggagctgga
     1501 gtcaactaaa gctgttatta tgggagaaac tgctgttgta gaaatagata cctttccccg
     1561 aggagtaatt ttaatatacc tagttatagc attttcacct tttggatact ttttggcaat
     1621 tcatatcgta gcagaaaaag aaaaaaaaat aaaagaattt ttaaagataa tgggacttca
     1681 tgatactgcc ttttggcttt cctgggttct tctatataca agtttaattt ttcttatgtc
     1741 ccttcttatg gcagtcattg cgacagcttc tttgttattt cctcaaagta gcagcattgt
     1801 gatatttctg ctttttttcc tttatggatt atcatctgta ttttttgctt taatgctgac
     1861 acctcttttt aaaaaatcaa aacatgtggg aatagttgaa ttttttgtta ctgtggcttt
     1921 tggatttatt ggccttatga taatcctcat agaaagtttt cccaaatcgt tagtgtggct
     1981 tttcagtcct ttctgtcact gtacttttgt gattggtatt gcacaggtca tgcatttaga
     2041 agattttaat gaaggtgctt cattttcaaa tttgactgca ggcccatatc ctctaattat
     2101 tacaattatc atgctcacac ttaatagtat attctatgtc ctcttggctg tctatcttga
     2161 tcaagtcatt ccaggggaat ttggcttacg gagatcatct ttatattttc tgaagccttc
     2221 atattggtca aagagcaaaa gaaattatga ggagttatca gagggcaatg ttaatggaaa
     2281 tattagtttt agtgaaatta ttgagccagt ttcttcagaa tttgtaggaa aagaagccat
     2341 aagaattagt ggtattcaga agacatacag aaagaagggt gaaaatgtgg aggctttgag
     2401 aaatttgtca tttgacatat atgagggtca gattactgcc ttacttggcc acagtggaac
     2461 aggaaagagt acattgatga atattctttg tggactctgc ccaccttctg atgggtttgc
     2521 atctatatat ggacacagag tctcagaaat agatgaaatg tttgaagcaa gaaaaatgat
     2581 tggcatttgt ccacagttag atatacactt tgatgttttg acagtagaag aaaatttatc
     2641 aattttggct tcaatcaaag ggataccagc caacaatata atacaagaag tgcagaaggt
     2701 tttactagat ttagacatgc agactatcaa agataaccaa gctaaaaaat taagtggtgg
     2761 tcaaaaaaga aagctgtcat taggaattgc tgttcttggg aacccaaaga tactgctgct
     2821 agatgaacca acagctggaa tggacccctg ttctcgacat attgtatgga atcttttaaa
     2881 atacagaaaa gccaatcggg tgacagtgtt cagtactcat ttcatggatg aagctgacat
     2941 tcttgcagat aggaaagctg tgatatcaca aggaatgctg aaatgtgttg gttcttcaat
     3001 gttcctcaaa agtaaatggg ggatcggcta ccgcctgagc atgtacatag acaaatattg
     3061 tgccacagaa tctctttctt cactggttaa acaacatata cctggagcta ctttattaca
     3121 acagaatgac caacaacttg tgtatagctt gcctttcaag gacatggaca aattttcagg
     3181 tttgttttct gccctagaca gtcattcaaa tttgggtgtc atttcttatg gtgtttccat
     3241 gacgactttg gaagacgtat ttttaaagct agaagttgaa gcagaaattg accaagcaga
     3301 ttatagtgta tttactcagc agccactgga ggaagaaatg gattcaaaat cttttgatga
     3361 aatggaacag agcttactta ttctttctga aaccaaggct gctctagtga gcaccatgag
     3421 cctttggaaa caacagatgt atacaatagc aaagtttcat ttctttacct tgaaacgtga
     3481 aagtaaatca gtgagatcag tgttgcttct gcttttaatt tttttcacag ttcagatttt
     3541 tatgtttttg gttcatcact cttttaaaaa tgctgtggtt cccatcaaac ttgttccaga
     3601 cttatatttt ctaaaacctg gagacaaacc acataaatac aaaacaagtc tgcttcttca
     3661 aaattctgct gactcagata tcagtgatct tattagcttt ttcacaagcc agaacataat
     3721 ggtgacgatg attaatgaca gtgactatgt atccgtggct ccccatagtg cggctttaaa
     3781 tgtgatgcat tcagaaaagg actatgtttt tgcagctgtt ttcaacagta ctatggttta
     3841 ttctttacct atattagtga atatcattag taactactat ctttatcatt taaatgtgac
     3901 tgaaaccatc cagatctgga gtaccccatt ctttcaagaa attactgata tagtttttaa
     3961 aattgagctg tattttcaag cagctttgct tggaatcatt gttactgcaa tgccacctta
     4021 ctttgccatg gaaaatgcag agaatcataa gatcaaagct tatactcaac ttaaactttc
     4081 aggtcttttg ccatctgcat attggattgg acaagctgtt gttgatatcc ccttattttt
     4141 tatcattctt attttgatgc taggaagctt attggcattt cattatggat tatattttta
     4201 tactgtaaag ttccttgctg tggttttttg ccttattggt tatgttccat cagttattct
     4261 gttcacttat attgcttctt tcacctttaa gaaaatttta aataccaaag aattttggtc
     4321 atttatctat tctgtggcag cgttggcttg tattgcaatc actgaaataa ctttctttat
     4381 gggatacaca attgcaacta ttcttcatta tgccttttgt atcatcattc caatctatcc
     4441 acttctaggt tgcctgattt ctttcataaa gatttcttgg aagaatgtac gaaaaaatgt
     4501 ggacacctat aatccatggg ataggctttc agtagctgtt atatcgcctt acctgcagtg
     4561 tgtactgtgg attttcctct tacaatacta tgagaaaaaa tatggaggca gatcaataag
     4621 aaaagatccc tttttcagaa acctttcaac gaagtctaaa aataggaagc ttccagaacc
     4681 accagacaat gaggatgaag atgaagatgt caaagctgaa agactaaagg tcaaagagct
     4741 gatgggttgc cagtgttgtg aggagaaacc atccattatg gtcagcaatt tgcataaaga
     4801 atatgatgac aagaaagatt ttcttctttc aagaaaagta aagaaagtgg caactaaata
     4861 catctctttc tgtgtgaaaa aaggagagat cttaggacta ttgggtccaa atggtgctgg
     4921 caaaagcaca attattaata ttctggttgg tgatattgaa ccaacttcag gccaggtatt
     4981 tttaggagat tattcttcag agacaagtga agatgatgat tcactgaagt gtatgggtta
     5041 ctgtcctcag ataaaccctt tgtggccaga tactacattg caggaacatt ttgaaattta
     5101 tggagctgtc aaaggaatga gtgcaagtga catgaaagaa gtcataagtc gaataacaca
     5161 tgcacttgat ttaaaagaac atcttcagaa gactgtaaag aaactacctg caggaatcaa
     5221 acgaaagttg tgttttgctc taagtatgct agggaatcct cagattactt tgctagatga
     5281 accatctaca ggtatggatc ccaaagccaa acagcacatg tggcgagcaa ttcgaactgc
     5341 atttaaaaac agaaagcggg ctgctattct gaccactcac tatatggagg aggcagaggc
     5401 tgtctgtgat cgagtagcta tcatggtgtc tgggcagtta agatgtatcg gaacagtaca
     5461 acatctaaag agtaaatttg gaaaaggcta ctttttggaa attaaattga aggactggat
     5521 agaaaaccta gaagtagacc gccttcaaag agaaattcag tatattttcc caaatgcaag
     5581 ccgtcaggaa agtttttctt ctattttggc ttataaaatt cctaaggaag atgttcagtc
     5641 cctttcacaa tcttttttta agctggaaga agctaaacat gcttttgcca ttgaagaata
     5701 tagcttttct caagcaacat tggaacaggt ttttgtagaa ctcactaaag aacaagagga
     5761 ggaagataat agttgtggaa ctttaaacag cacactttgg tgggaacgaa cacaagaaga
     5821 tagagtagta ttttgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     5881 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5941 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6001 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6061 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6121 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6361 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6841 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6901 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6961 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7021 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7081 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7141 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     7201 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     7261 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7321 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7381 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7441 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7501 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7561 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7621 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7681 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7741 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7801 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7861 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7921 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8101 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9061 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9121 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9181 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9241 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9301 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9361 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9421 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9481 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9541 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9601 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9661 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9721 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9781 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9841 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9901 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9961 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10021 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10081 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10141 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10201 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10261 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
