LOCUS       V028769                 9250 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028769
VERSION     V028769
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4770
                     /label="ABCB1(NM_000927)"
                     /note="ABCB1(NM_000927)"
                     /gene="ABCB1"
     polyA_signal    4817..5041
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5087..5515
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5529..5858
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5925..6719
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6893..7014
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7063..7079)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7087..7103
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7111..7141)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7156..7177
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7465..8050)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8221..9081)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9082..9186)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggatcttg aaggggaccg caatggagga
      961 gcaaagaaga agaacttttt taaactgaac aataaaagtg aaaaagataa gaaggaaaag
     1021 aaaccaactg tcagtgtatt ttcaatgttt cgctattcaa attggcttga caagttgtat
     1081 atggtggtgg gaactttggc tgccatcatc catggggctg gacttcctct catgatgctg
     1141 gtgtttggag aaatgacaga tatctttgca aatgcaggaa atttagaaga tctgatgtca
     1201 aacatcacta atagaagtga tatcaatgat acagggttct tcatgaatct ggaggaagac
     1261 atgaccaggt atgcctatta ttacagtgga attggtgctg gggtgctggt tgctgcttac
     1321 attcaggttt cattttggtg cctggcagct ggaagacaaa tacacaaaat tagaaaacag
     1381 ttttttcatg ctataatgcg acaggagata ggctggtttg atgtgcacga tgttggggag
     1441 cttaacaccc gacttacaga tgatgtctcc aagattaatg aaggaattgg tgacaaaatt
     1501 ggaatgttct ttcagtcaat ggcaacattt ttcactgggt ttatagtagg atttacacgt
     1561 ggttggaagc taacccttgt gattttggcc atcagtcctg ttcttggact gtcagctgct
     1621 gtctgggcaa agatactatc ttcatttact gataaagaac tcttagcgta tgcaaaagct
     1681 ggagcagtag ctgaagaggt cttggcagca attagaactg tgattgcatt tggaggacaa
     1741 aagaaagaac ttgaaaggta caacaaaaat ttagaagaag ctaaaagaat tgggataaag
     1801 aaagctatta cagccaatat ttctataggt gctgctttcc tgctgatcta tgcatcttat
     1861 gctctggcct tctggtatgg gaccaccttg gtcctctcag gggaatattc tattggacaa
     1921 gtactcactg tattcttttc tgtattaatt ggggctttta gtgttggaca ggcatctcca
     1981 agcattgaag catttgcaaa tgcaagagga gcagcttatg aaatcttcaa gataattgat
     2041 aataagccaa gtattgacag ctattcgaag agtgggcaca aaccagataa tattaaggga
     2101 aatttggaat tcagaaatgt tcacttcagt tacccatctc gaaaagaagt taagatcttg
     2161 aagggtctga acctgaaggt gcagagtggg cagacggtgg ccctggttgg aaacagtggc
     2221 tgtgggaaga gcacaacagt ccagctgatg cagaggctct atgaccccac agaggggatg
     2281 gtcagtgttg atggacagga tattaggacc ataaatgtaa ggtttctacg ggaaatcatt
     2341 ggtgtggtga gtcaggaacc tgtattgttt gccaccacga tagctgaaaa cattcgctat
     2401 ggccgtgaaa atgtcaccat ggatgagatt gagaaagctg tcaaggaagc caatgcctat
     2461 gactttatca tgaaactgcc tcataaattt gacaccctgg ttggagagag aggggcccag
     2521 ttgagtggtg ggcagaagca gaggatcgcc attgcacgtg ccctggttcg caaccccaag
     2581 atcctcctgc tggatgaggc cacgtcagcc ttggacacag aaagcgaagc agtggttcag
     2641 gtggctctgg ataaggccag aaaaggtcgg accaccattg tgatagctca tcgtttgtct
     2701 acagttcgta atgctgacgt catcgctggt ttcgatgatg gagtcattgt ggagaaagga
     2761 aatcatgatg aactcatgaa agagaaaggc atttacttca aacttgtcac aatgcagaca
     2821 gcaggaaatg aagttgaatt agaaaatgca gctgatgaat ccaaaagtga aattgatgcc
     2881 ttggaaatgt cttcaaatga ttcaagatcc agtctaataa gaaaaagatc aactcgtagg
     2941 agtgtccgtg gatcacaagc ccaagacaga aagcttagta ccaaagaggc tctggatgaa
     3001 agtatacctc cagtttcctt ttggaggatt atgaagctaa atttaactga atggccttat
     3061 tttgttgttg gtgtattttg tgccattata aatggaggcc tgcaaccagc atttgcaata
     3121 atattttcaa agattatagg ggtttttaca agaattgatg atcctgaaac aaaacgacag
     3181 aatagtaact tgttttcact attgtttcta gcccttggaa ttatttcttt tattacattt
     3241 ttccttcagg gtttcacatt tggcaaagct ggagagatcc tcaccaagcg gctccgatac
     3301 atggttttcc gatccatgct cagacaggat gtgagttggt ttgatgaccc taaaaacacc
     3361 actggagcat tgactaccag gctcgccaat gatgctgctc aagttaaagg ggctataggt
     3421 tccaggcttg ctgtaattac ccagaatata gcaaatcttg ggacaggaat aattatatcc
     3481 ttcatctatg gttggcaact aacactgtta ctcttagcaa ttgtacccat cattgcaata
     3541 gcaggagttg ttgaaatgaa aatgttgtct ggacaagcac tgaaagataa gaaagaacta
     3601 gaaggttctg ggaagatcgc tactgaagca atagaaaact tccgaaccgt tgtttctttg
     3661 actcaggagc agaagtttga acatatgtat gctcagagtt tgcaggtacc atacagaaac
     3721 tctttgagga aagcacacat ctttggaatt acattttcct tcacccaggc aatgatgtat
     3781 ttttcctatg ctggatgttt ccggtttgga gcctacttgg tggcacataa actcatgagc
     3841 tttgaggatg ttctgttagt attttcagct gttgtctttg gtgccatggc cgtggggcaa
     3901 gtcagttcat ttgctcctga ctatgccaaa gccaaaatat cagcagccca catcatcatg
     3961 atcattgaaa aaaccccttt gattgacagc tacagcacgg aaggcctaat gccgaacaca
     4021 ttggaaggaa atgtcacatt tggtgaagtt gtattcaact atcccacccg accggacatc
     4081 ccagtgcttc agggactgag cctggaggtg aagaagggcc agacgctggc tctggtgggc
     4141 agcagtggct gtgggaagag cacagtggtc cagctcctgg agcggttcta cgaccccttg
     4201 gcagggaaag tgctgcttga tggcaaagaa ataaagcgac tgaatgttca gtggctccga
     4261 gcacacctgg gcatcgtgtc ccaggagccc atcctgtttg actgcagcat tgctgagaac
     4321 attgcctatg gagacaacag ccgggtggtg tcacaggaag agattgtgag ggcagcaaag
     4381 gaggccaaca tacatgcctt catcgagtca ctgcctaata aatatagcac taaagtagga
     4441 gacaaaggaa ctcagctctc tggtggccag aaacaacgca ttgccatagc tcgtgccctt
     4501 gttagacagc ctcatatttt gcttttggat gaagccacgt cagctctgga tacagaaagt
     4561 gaaaaggttg tccaagaagc cctggacaaa gccagagaag gccgcacctg cattgtgatt
     4621 gctcaccgcc tgtccaccat ccagaatgca gacttaatag tggtgtttca gaatggcaga
     4681 gtcaaggagc atggcacgca tcagcagctg ctggcacaga aaggcatcta tttttcaatg
     4741 gtcagtgtcc aggctggaac aaagcgccag tgactcgagt ctagagggcc cgtttaaacc
     4801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5041 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5101 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5161 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5221 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5281 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5341 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5401 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5461 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5521 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5581 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5701 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5761 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5821 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5881 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5941 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     6001 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     6061 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     6121 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6181 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6241 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6301 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6361 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6421 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6481 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6541 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6601 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6661 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6721 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6781 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6841 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7021 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7981 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9181 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     9241 cccctatggt
//
