LOCUS V019787 7247 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019787 VERSION V019787 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2737 /label="ABCE1(NM_001040876)" /note="ABCE1(NM_001040876)" /gene="ABCE1" CDS 2744..2809 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2847..3071 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3117..3545 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3559..3888 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3955..4749 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4923..5044 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5093..5109) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5117..5133 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5141..5171) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5186..5207 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5495..6080) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6251..7111) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7112..7216) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcagaca agttaacgag 961 aattgctatt gtcaaccatg acaaatgtaa acctaagaaa tgtcgacagg aatgcaaaaa 1021 gagttgtcct gtagttcgaa tgggaaaatt atgcatagag gttacacccc agagcaaaat 1081 agcatggatt tccgaaactc tttgtattgg ttgtggtatc tgtattaaga aatgcccctt 1141 tggcgcctta tcaattgtca atctaccaag caacttggaa aaagaaacca cacatcgata 1201 ttgtgccaat gccttcaaac ttcacaggtt gcctatccct cgtccaggtg aagttttggg 1261 attagttgga actaatggta ttggaaagtc aactgcttta aaaattttag caggaaaaca 1321 aaagccaaac cttggaaagt acgatgatcc tcctgactgg caggagattt tgacttattt 1381 ccgtggatct gaattacaaa attactttac aaagattcta gaagatgacc taaaagccat 1441 catcaaacct caatatgtag accagattcc taaggctgca aaggggacag tgggatctat 1501 tttggaccga aaagatgaaa caaagacaca ggcaattgta tgtcagcagc ttgatttaac 1561 ccacctaaaa gaacgaaatg ttgaagatct ttcaggagga gagttgcaga gatttgcttg 1621 tgctgtcgtt tgcatacaga aagctgatat tttcatgttt gatgagcctt ctagttacct 1681 agatgtcaag cagcgtttaa aggctgctat tactatacga tctctaataa atccagatag 1741 atatatcatt gtggtggaac atgatctaag tgtattagac tatctctccg acttcatctg 1801 ctgtttatat ggtgtaccaa gcgcctatgg agttgtcact atgcctttta gtgtaagaga 1861 aggcataaac atttttttgg atggctatgt tccaacagaa aacttgagat tcagagatgc 1921 atcacttgtt tttaaagtgg ctgagacagc aaatgaagaa gaagttaaaa agatgtgtat 1981 gtataaatat ccaggaatga agaaaaaaat gggagaattt gagctagcaa ttgtagctgg 2041 agagtttaca gattctgaaa ttatggtgat gctgggggaa aatggaacgg gtaaaacgac 2101 atttatcaga atgcttgctg gaagacttaa acctgatgaa ggaggagaag taccagttct 2161 aaatgtcagt tataagccac agaaaattag tcccaaatca actggaagtg ttcgccagtt 2221 actacatgaa aagataagag atgcttatac tcacccacaa tttgtgaccg atgtaatgaa 2281 gcctctgcaa attgaaaaca tcattgatca agaggtgcag acattatctg gtggtgaact 2341 acagcgagta gctttagccc tttgcttggg caaacctgct gatgtctatt taattgatga 2401 accatctgca tatttggatt ctgagcaaag actgatggca gctcgagttg tcaaacgttt 2461 catactccat gcaaaaaaga cagcctttgt tgtggaacat gacttcatca tggccaccta 2521 tctagcggat cgcgtcatcg tttttgatgg tgttccatct aagaacacag ttgcaaacag 2581 tcctcaaacc cttttggctg gcatgaataa atttttgtct cagcttgaaa ttacattcag 2641 aagagatcca aacaactata ggccacgaat aaacaaactt aattcaatta aggatgtaga 2701 acaaaagaag agtggaaact actttttctt ggatgatctc gaggactaca aagaccatga 2761 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc 2821 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 2881 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 2941 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3001 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3061 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3541 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 3601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 3661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 3721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 3781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 3841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 3901 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 3961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 4021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 4081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 4141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 4201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 4261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 4321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 4381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 4441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 4501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 4561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 4621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 4681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 4741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 4801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 4921 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 4981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5041 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 5341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 5821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 5881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 5941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6001 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 6061 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6121 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6181 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6241 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6301 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6361 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6421 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6481 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6541 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6601 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6661 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6721 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 6781 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 6841 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 6901 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6961 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7021 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7081 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //