LOCUS V018657 7518 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018657 VERSION V018657 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 890..3031 /label="AGGF1(NM_018046)" /note="AGGF1(NM_018046)" /gene="AGGF1" polyA_signal 3085..3309 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3355..3783 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3797..4126 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4193..4987 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5161..5282 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5331..5347) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5355..5371 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5379..5409) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5424..5445 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5733..6318) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6489..7349) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7350..7454) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tggcctcgga 901 ggcgccgtcc ccgccgcggt cgccgccgcc gcccacctcc cccgagcctg agctggccca 961 gctaaggcgg aaggtggaga agttggaacg tgaactgcgg agctgcaagc ggcaggtgcg 1021 ggagatcgag aagctgctgc atcacacaga acggctgtac cagaacgcag aaagcaacaa 1081 ccaggagctc cgcacgcagg tggaagaact cagtaaaata ctccaacgtg ggagaaatga 1141 agataataaa aagtctgatg tagaagtaca aacagagaac catgctcctt ggtcaatctc 1201 agattatttt tatcagacgt actacaatga cgttagtctt ccaaataaag tgactgaact 1261 gtcagatcaa caagatcaag ctatcgaaac ttctattttg aattctaaag accatttaca 1321 agtagaaaat gatgcttacc ctggtaccga tagaacagaa aatgttaaat atagacaagt 1381 ggaccatttt gcctcaaatt cacaggagcc agcatctgca ttagcaacag aagatacctc 1441 cttagaaggc tcatcattag ctgaaagttt gagagctgca gcagaagcgg ctgtatcaca 1501 gactggattt agttatgatg aaaatactgg actgtatttt gaccacagca ctggtttcta 1561 ttatgattct gaaaatcaac tctattatga tccttccact ggaatttatt actattgtga 1621 tgtggaaagt ggtcgttatc agtttcattc tcgagtagat ttgcaacctt atccgacttc 1681 tagcacaaaa caaagtaaag ataaaaaatt gaagaagaaa agaaaagatc cagattcttc 1741 tgcaacaaat gaggaaaagg atttgaactc agaggatcaa aaagccttca gtgttgaaca 1801 tacaagctgc aatgaggaag aaaatttcgc aaatatgaaa aagaaggcca aaataggcat 1861 tcatcacaaa aatagtcccc ccaaagtcac tgttccaact agtggaaata ctatagagtc 1921 tcctcttcat gaaaacatct ctaattcaac atcatttaaa gatgagaaaa tcatggagac 1981 tgatagtgaa ccagaggaag gtgaaattac agactctcag actgaggata gttatgacga 2041 agccattacc agtgaaggca atgtaactgc agaagatagt gaggatgaag atgaagacaa 2101 aatttggccc ccatgtatta gagtaattgt cattagatca cctgtgttgc agataggatc 2161 actctttatc attactgctg taaaccctgc tacaattgga agagaaaagg atatggaaca 2221 tactctccga atccctgaag ttggtgtcag taagtttcat gcagaaattt attttgacca 2281 tgacttacaa agttatgtcc ttgtggatca aggcagtcaa aatggcacaa ttgttaatgg 2341 aaaacagatt cttcagccga aaactaaatg tgacccttac gtacttgagc atggagatga 2401 agtcaaaatt ggagaaactg tcttatcctt tcacattcat cctggcagtg atacctgtga 2461 tggctgtgaa ccagggcagg ttagagccca ccttcgcctt gataagaaag atgaatcttt 2521 tgttggtcca acactaagta aggaggaaaa agagttggaa agaagaaaag aattaaagaa 2581 aatacgagta aaatatggtt tacagaatac agaatacgaa gatgaaaaga cattgaagaa 2641 tccaaaatat aaagatagag ctggaaaacg tagggagcag gttggaagtg aaggaacttt 2701 ccaaagagat gatgctcctg catctgttca ttctgaaatt actgatagca acaaaggtcg 2761 gaagatgttg gagaagatgg gttggaagaa aggagagggc ctggggaagg atggtggagg 2821 aatgaaaacg ccgatccagc ttcagcttcg gcgaacacat gcaggcttgg ggacaggcaa 2881 accatcctca tttgaagatg ttcaccttct ccaaaacaag aacaaaaaaa actgggacaa 2941 agcacgagag cggtttactg aaaacttccc agaaactaag cctcaaaaag atgacccagg 3001 gaccatgcct tgggtaaaag ggactttaga gtgagcggcc gctcgagtct agagggcccg 3061 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3301 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 3361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3421 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3541 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 3601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 3661 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 3721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 3781 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 3841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 3901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 3961 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4081 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4141 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4201 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4261 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4321 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4381 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4441 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4501 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4561 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4621 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4681 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 4741 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4801 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4861 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4921 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 4981 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5041 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5101 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5281 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 5341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 5401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 5461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 5521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 5581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 5641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 5701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 5761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 5821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 6181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6241 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 6301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6361 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 6541 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 6601 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 6661 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 6721 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 6781 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 6841 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 6901 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 6961 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7021 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7081 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7141 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7201 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7261 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7321 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7381 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7441 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 7501 ctcccgatcc cctatggt //