LOCUS       V047348                 7993 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047348
VERSION     V047348
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             950..3493
                     /label="AHR(NM_001621)"
                     /note="AHR(NM_001621)"
                     /gene="AHR"
     CDS             3500..3526
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        complement(3545..3563)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     polyA_signal    3589..3813
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3859..4287
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4301..4631
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4698..5492
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5666..5787
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5836..5852)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5860..5876
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5884..5914)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5929..5950
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6238..6826)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6997..7857)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7858..7962)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgaacagcag
      961 cagcgccaac atcacctacg ccagtcgcaa gcggcggaag ccggtgcaga aaacagtaaa
     1021 gccaatccca gctgaaggaa tcaagtcaaa tccttccaag cggcatagag accgacttaa
     1081 tacagagttg gaccgtttgg ctagcctgct gcctttccca caagatgtta ttaataagtt
     1141 ggacaaactt tcagttctta ggctcagcgt cagttacctg agagccaaga gcttctttga
     1201 tgttgcatta aaatcctccc ctactgaaag aaacggaggc caggataact gtagagcagc
     1261 aaatttcaga gaaggcctga acttacaaga aggagaattc ttattacagg ctctgaatgg
     1321 ctttgtatta gttgtcacta cagatgcttt ggtcttttat gcttcttcta ctatacaaga
     1381 ttatctaggg tttcagcagt ctgatgtcat acatcagagt gtatatgaac ttatccatac
     1441 cgaagaccga gctgaatttc agcgtcagct acactgggca ttaaatcctt ctcagtgtac
     1501 agagtctgga caaggaattg aagaagccac tggtctcccc cagacagtag tctgttataa
     1561 cccagaccag attcctccag aaaactctcc tttaatggag aggtgcttca tatgtcgtct
     1621 aaggtgtctg ctggataatt catctggttt tctggcaatg aatttccaag ggaagttaaa
     1681 gtatcttcat ggacagaaaa agaaagggaa agatggatca atacttccac ctcagttggc
     1741 tttgtttgcg atagctactc cacttcagcc accatccata cttgaaatcc ggaccaaaaa
     1801 ttttatcttt agaaccaaac acaaactaga cttcacacct attggttgtg atgccaaagg
     1861 aagaattgtt ttaggatata ctgaagcaga gctgtgcacg agaggctcag gttatcagtt
     1921 tattcatgca gctgatatgc tttattgtgc cgagtcccat atccgaatga ttaagactgg
     1981 agaaagtggc atgatagttt tccggcttct tacaaaaaac aaccgatgga cttgggtcca
     2041 gtctaatgca cgcctgcttt ataaaaatgg aagaccagat tatatcattg taactcagag
     2101 accactaaca gatgaggaag gaacagagca tttacgaaaa cgaaatacga agttgccttt
     2161 tatgtttacc actggagaag ctgtgttgta tgaggcaacc aacccttttc ctgccataat
     2221 ggatccctta ccactaagga ctaaaaatgg cactagtgga aaagactctg ctaccacatc
     2281 cactctaagc aaggactctc tcaatcctag ttccctcctg gctgccatga tgcaacaaga
     2341 tgagtctatt tatctctatc ctgcttcaag tacttcaagt actgcacctt ttgaaaacaa
     2401 ctttttcaac gaatctatga atgaatgcag aaattggcaa gataatactg caccgatggg
     2461 aaatgatact atcctgaaac atgagcaaat tgaccagcct caggatgtga actcatttgc
     2521 tggaggtcac ccagggctct ttcaagatag taaaaacagt gacttgtaca gcataatgaa
     2581 aaacctaggc attgattttg aagacatcag acacatgcag aatgaaaaat ttttcagaaa
     2641 tgatttttct ggtgaggttg acttcagaga cattgactta acggatgaaa tcctgacgta
     2701 tgtccaagat tctttaagta agtctccctt cataccttca gattatcaac agcaacagtc
     2761 cttggctctg aactcaagct gtatggtaca ggaacaccta catctagaac agcaacagca
     2821 acatcaccaa aagcaagtag tagtggagcc acagcaacag ctgtgtcaga agatgaagca
     2881 catgcaagtt aatggcatgt ttgaaaattg gaactctaac caattcgtgc ctttcaattg
     2941 tccacagcaa gacccacaac aatataatgt ctttacagac ttacatggga tcagtcaaga
     3001 gttcccctac aaatctgaaa tggattctat gccttataca cagaacttta tttcctgtaa
     3061 tcagcctgta ttaccacaac attccaaatg tacagagctg gactacccta tggggagttt
     3121 tgaaccatcc ccatacccca ctacttctag tttagaagat tttgtcactt gtttacaact
     3181 tcctgaaaac caaaagcatg gattaaatcc acagtcagcc ataataactc ctcagacatg
     3241 ttatgctggg gccgtgtcga tgtatcagtg ccagccagaa cctcagcaca cccacgtggg
     3301 tcagatgcag tacaatccag tactgccagg ccaacaggca tttttaaaca agtttcagaa
     3361 tggagtttta aatgaaacat atccagctga attaaataac ataaataaca ctcagactac
     3421 cacacatctt cagccacttc atcatccgtc agaagccaga ccttttcctg atttgacatc
     3481 cagtggattc ctgctcgagt acccatacga cgtaccagat tacgcttagt ctagagggcc
     3541 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     3601 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3661 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3721 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3781 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3841 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3901 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3961 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     4021 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4081 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4141 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4201 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     4261 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4321 aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4381 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4441 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4501 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4561 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4621 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     4681 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4741 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4801 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4861 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4921 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4981 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5041 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5101 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5161 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5221 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5281 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5341 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5401 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5461 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5521 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5581 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5641 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5701 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5761 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5821 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5881 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5941 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6001 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6061 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6421 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6901 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6961 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7021 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7081 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7141 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7201 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7261 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7321 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7381 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7441 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7501 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7561 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7621 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7681 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7741 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7801 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7861 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7921 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7981 gccacctgac gtc
//
