LOCUS V028429 6529 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028429 VERSION V028429 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2001 /label="ANXA2(NM_001002858)" /note="ANXA2(NM_001002858)" /gene="ANXA2" CDS 2008..2049 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" polyA_signal 2096..2320 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2366..2794 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2808..3137 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3204..3998 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4172..4293 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4342..4358) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4366..4382 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4390..4420) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4435..4456 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4744..5329) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5500..6360) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6361..6465) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgggccgcc agctagcggg gtgtggagac 961 gctgggaaga aggcttcctt caaaatgtct actgttcacg aaatcctgtg caagctcagc 1021 ttggagggtg atcactctac acccccaagt gcatatgggt ctgtcaaagc ctatactaac 1081 tttgatgctg agcgggatgc tttgaacatt gaaacagcca tcaagaccaa aggtgtggat 1141 gaggtcacca ttgtcaacat tttgaccaac cgcagcaatg cacagagaca ggatattgcc 1201 ttcgcctacc agagaaggac caaaaaggaa cttgcatcag cactgaagtc agccttatct 1261 ggccacctgg agacggtgat tttgggccta ttgaagacac ctgctcagta tgacgcttct 1321 gagctaaaag cttccatgaa ggggctggga accgacgagg actctctcat tgagatcatc 1381 tgctccagaa ccaaccagga gctgcaggaa attaacagag tctacaagga aatgtacaag 1441 actgatctgg agaaggacat tatttcggac acatctggtg acttccgcaa gctgatggtt 1501 gccctggcaa agggtagaag agcagaggat ggctctgtca ttgattatga actgattgac 1561 caagatgctc gggatctcta tgacgctgga gtgaagagga aaggaactga tgttcccaag 1621 tggatcagca tcatgaccga gcggagcgtg ccccacctcc agaaagtatt tgataggtac 1681 aagagttaca gcccttatga catgttggaa agcatcagga aagaggttaa aggagacctg 1741 gaaaatgctt tcctgaacct ggttcagtgc attcagaaca agcccctgta ttttgctgat 1801 cggctgtatg actccatgaa gggcaagggg acgcgagata aggtcctgat cagaatcatg 1861 gtctcccgca gtgaagtgga catgttgaaa attaggtctg aattcaagag aaagtacggc 1921 aagtccctgt actattatat ccagcaagac actaagggcg actaccagaa agcgctgctg 1981 tacctgtgtg gtggagatga cgatatcggt aagcctatcc ctaaccctct cctcggtctc 2041 gattctacgt gactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 2101 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2161 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2221 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2281 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2341 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 2401 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2521 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 2581 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 2641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 2701 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 2761 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 2821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2881 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2941 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3061 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3121 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 3181 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3361 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 3421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3721 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4141 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4321 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4381 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4441 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4501 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 5641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 5701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6481 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt //