LOCUS V042492 5964 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042492 VERSION V042492 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 955..1479 /label="ARF6(NM_001663)" /note="ARF6(NM_001663)" /gene="ARF6" CDS 1501..1530 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 1559..1783 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1829..2257 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2271..2600 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2667..3461 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3637..3758 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3807..3823) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3831..3847 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3855..3885) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3900..3921 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4209..4797) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4968..5828) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5829..5933) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatgggg 961 aaggtgctat ccaaaatctt cgggaacaag gaaatgcgga tcctcatgtt gggcctggac 1021 gcggccggca agacaacaat cctgtacaag ttgaagctgg gccagtcggt gaccaccatt 1081 cccactgtgg gtttcaacgt ggagacggtg acttacaaaa atgtcaagtt caacgtatgg 1141 gatgtgggcg gccaggacaa gatccggccg ctctggcggc attactacac tgggacccaa 1201 ggtctcatct tcgtagtgga ctgcgccgac cgcgaccgca tcgatgaggc tcgccaggag 1261 ctgcaccgca ttatcaatga ccgggagatg agggacgcca taatcctcat cttcgccaac 1321 aagcaggacc tgcccgatgc catgaaaccc cacgagatcc aggagaaact gggcctgacc 1381 cggattcggg acaggaactg gtatgtgcag ccctcctgtg ccacctcagg ggacggactc 1441 tatgaggggc tcacatggtt aacctctaac tacaaatctc tcgagtctag agggcccttc 1501 gaacaaaaac tcatctcaga agaggatctg tgagtttaaa cccgctgatc agcctcgact 1561 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 1621 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 1681 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 1741 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 1801 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 1861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 1921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 1981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 2041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 2101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 2161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 2221 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 2281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 2461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2521 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 2581 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 2641 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 2701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 2761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 2821 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 2881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 2941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 3001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 3061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 3121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 3181 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 3241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 3301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 3361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 3421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc 3481 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 3541 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 3601 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 3661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 3781 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3961 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 4021 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 4081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 4141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 4201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 4261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 4321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 4381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 4441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4621 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 4681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 5041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 5101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 5161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 5221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 5281 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 5341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 5401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 5461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 5521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 5581 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5821 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5941 ccccgaaaag tgccacctga cgtc //