LOCUS       V018339                 8267 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018339
VERSION     V018339
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             890..3673
                     /label="BCAS3(NM_001099432)"
                     /note="BCAS3(NM_001099432)"
                     /gene="BCAS3"
     CDS             3713..3739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3743..3769
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3773..3799
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3834..4058
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4104..4532
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4546..4875
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4942..5736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5910..6031
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6080..6096)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6104..6120
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6128..6158)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6173..6194
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6482..7067)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7238..8098)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8099..8203)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgaatgaagc
      901 tatggctaca gattccccaa gaagacccag tcgttgtact ggtggagttg tggttcgccc
      961 ccaggctgtc acagagcagt cctacatgga aagtgttgtg acttttctgc aggatgttgt
     1021 gccacaggct tacagtggaa cacctctaac agaagaaaag gagaaaatag tctgggtcag
     1081 atttgaaaat gcagatttaa atgatacatc aagaaatctg gaatttcatg aaatacatag
     1141 tactgggaat gaaccgcctt tgttgattat gattggctac agtgatggaa tgcaggtctg
     1201 gagcatccct atcagtggtg aagcacaaga gctcttctct gttcgacatg gcccaattcg
     1261 agcggctaga atcttgcctg ctccacagtt tggtgctcaa aaatgtgata actttgctga
     1321 aaaaagaccc ctccttggtg tttgtaagag cattggatct tctggcacaa gcccaccgta
     1381 ctgttgtgtg gatctgtatt cacttcgtac tggggagatg gtcaagtcca ttcaatttaa
     1441 gacacctatt tatgatctcc attgcaataa acggatcctt gtcgtagtct tgcaggagaa
     1501 aattgctgcc tttgatagct gtactttcac gaagaaattc tttgttacaa gctgctatcc
     1561 atgtccaggg ccaaacatga atcctattgc tcttgggagc cgctggcttg cttatgcaga
     1621 aaacaagttg attcgatgtc atcagtcccg tggtggagcc tgtggagaca acattcagtc
     1681 ttatactgcc acagtcatta gtgctgctaa aacattgaaa agtggcctga caatggtagg
     1741 gaaagtggtg actcagctga caggcacact gccttcaggt gtgacagaag atgatgttgc
     1801 catccacagt aattcacggc ggagtccttt ggtcccaggc atcatcacag ttattgacac
     1861 cgaaaccgtt ggagagggcc aggtgcttgt gagtgaggat tctgacagtg atggcattgt
     1921 ggcccacttc cctgcccatg agaagccagt gtgctgcatg gcttttaata caagtggaat
     1981 gcttctagtc acaacagaca cccttggcca tgactttcat gtcttccaaa ttctgactca
     2041 tccttggtcc tcatcacaat gtgctgtcca ccatctgtat actcttcaca ggggagaaac
     2101 tgaagccaaa gtacaggaca tctgcttcag ccatgactgt cgctgggttg tggtcagtac
     2161 tctccggggt acttcccacg ttttccccat caacccttat ggtggccagc cttgtgttcg
     2221 tacacatatg tcaccacgag tagtgaatcg catgagccgt ttccagaaaa gtgctggact
     2281 ggaagagatt gaacaagaac tgacgtctaa gcaaggaggt cgctgtagcc ctgttccagg
     2341 tctatcaagc agcccttctg ggtcaccctt gcatgggaaa ctgaacagcc aagactccta
     2401 taacaatttt accaacaaca accctggcaa ccctcggctc tctcctcttc ccagcttgat
     2461 ggtagtgatg cctcttgcac aaatcaagca gccaatgaca ttggggacca tcaccaaacg
     2521 aaccgggcct tatctctttg gagcggggtg tttttccata aaagccccat gcaaagttaa
     2581 acctcctcca caaatttcac ccagcaaatc gatgggcgga gaattttgtg tggctgctat
     2641 cttcggaaca tccaggtcat ggtttgcaaa taatgcaggt ctgaaaagag aaaaagatca
     2701 gtccaaacaa gttgtagttg agtccctgta cattatcagt tgctatggca ccttagtgga
     2761 acacatgatg gagccgcgac ccctcagcac tgcacccaag attagtgacg acacaccact
     2821 ggaaatgatg acatcgcctc gagccagctg gactctggtt agaacccctc aatggaatga
     2881 attgcagcca ccgtttaatg caaaccaccc tctgctcctc gctgcagatg cagtacagta
     2941 ttatcagttc ctgcttgctg gcctggttcc ccctggaagt cctgggccca ttactcgaca
     3001 tgggtcttac gacagtttag cttctgacca tagtggacag gaagatgaag aatggctttc
     3061 ccaggttgaa attgtaacac acactggacc ccatagacgt ctgtggatgg gtccacagtt
     3121 ccagttcaaa accatccatc cctcaggcca aaccacagtt atctcatcca gttcatctgt
     3181 gttgcagtct catggtccga gtgacacgcc acagcctctt ttggattttg atacagatga
     3241 tcttgatctc aacagtctca ggatccagcc agtccgctct gaccccgtca gcatgccagg
     3301 gtcatcccgt ccagtctctg atcgaagggg agtttccaca gtgattgatg ctgcctcagg
     3361 tacctttgac aggagcgtga ccctgctgga ggtgtgcggg agctggcctg agggcttcgg
     3421 gctgcggcac atgtcctcca tggagcacac ggaggagggc ctccgggagc gacttgccga
     3481 cgccatggcc gagtcaccta gccgggacgt cgtgggatcc ggaacagaac ttcagcgaga
     3541 gggaagcatc gagactctga gtaacagctc aggctccacc agcggcagca taccaagaaa
     3601 ctttgatggc taccgatctc cgctgcccac caatgagagc cagcccctca gcctcttccc
     3661 gactggcttc ccggaattct gcagatatcc agcacagtgg cggccgctcg agtacccata
     3721 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     3781 tgatgtccca gactacgcat gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3841 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3901 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3961 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4021 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4081 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4141 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4201 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4261 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4321 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4381 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4441 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4501 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4561 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4621 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4681 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4741 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4801 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4861 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     4921 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4981 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5041 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5101 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5161 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5221 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5281 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5341 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5401 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5461 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5521 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5581 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5641 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5701 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5761 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5821 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5881 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     7021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7141 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7201 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7261 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7321 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7381 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7441 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7501 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7561 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7621 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7681 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7741 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7801 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7861 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7921 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7981 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8041 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8101 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8161 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8221 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
