LOCUS V030573 6248 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030573 VERSION V030573 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1741 /label="CD200(NM_001365851)" /note="CD200(NM_001365851)" /gene="CD200" CDS 1751..1768 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 1815..2039 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2085..2513 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2527..2856 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2923..3717 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3891..4012 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4061..4077) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4085..4101 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4109..4139) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4154..4175 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4463..5048) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5219..6079) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6080..6184) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gagaggctgg tgatcaggat gcccttctct catctgtcta cctacagcct 961 ggtttgggtc atggcagcag tggtgctgtg cacagcacaa gtgcaagtgg tgacccagga 1021 tgaaagagag cagctgtaca cacctgcttc cttaaaatgc tctctgcaaa atgcccagga 1081 agccctcatt gtgacatggc agaaaaagaa agctgtaagc ccagaaaaca tggtcacctt 1141 cagcgagaac catggggtgg tgatccagcc tgcctataag gacaagataa acattaccca 1201 gctgggactc caaaactcaa ccatcacctt ctggaatatc accctggagg atgaagggtg 1261 ttacatgtgt ctcttcaata cctttggttt tgggaagatc tcaggaacgg cctgcctcac 1321 cgtctatgta cagcccatag tatcccttca ctacaaattc tctgaagacc acctaaatat 1381 cacttgctct gccactgccc gcccagcccc catggtcttc tggaaggtcc ctcggtcagg 1441 gattgaaaat agtacagtga ctctgtctca cccaaatggg accacgtctg ttaccagcat 1501 cctccatatc aaagacccta agaatcaggt ggggaaggag gtgatctgcc aggtgctgca 1561 cctggggact gtgaccgact ttaagcaaac cgtcaacaaa ggctattggt tttcagttcc 1621 gctattgcta agcattgttt ccctggtaat tcttctcgtc ctaatctcaa tcttactgta 1681 ctggaaacgt caccggaatc aggaccgagg tgagttgtca cagggagttc aaaaaatgac 1741 agcggccgca catcaccatc atcaccattg actcgagtct agagggcccg tttaaacccg 1801 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 1861 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 1921 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 1981 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2041 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 2101 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2161 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2221 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2281 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 2341 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2401 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 2461 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 2521 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2581 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 2641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 2701 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 2761 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 2821 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 2881 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 2941 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 3001 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 3061 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 3121 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 3181 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 3241 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 3301 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 3361 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 3421 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 3481 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 3541 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 3601 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 3661 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 3721 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 3781 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 3841 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 3901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 3961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4021 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4861 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 4921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4981 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 5401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 5461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 5521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 5581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 5641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 5701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 5761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 5821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 5881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 5941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6181 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 6241 cctatggt //