LOCUS       V035296                10582 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035296
VERSION     V035296
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     promoter        890..908
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     CDS             918..6047
                     /label="CEP152(NM_001194998)"
                     /note="CEP152(NM_001194998)"
                     /gene="CEP152"
     polyA_signal    6149..6373
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6419..6847
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6861..7190
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7257..8051
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8225..8346
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8395..8411)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8419..8435
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8443..8473)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8488..8509
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8797..9382)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9553..10413)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10414..10518)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttcatacga tttaggtgac
      901 actatagaac cgccaccatg tcattagact ttggcagtgt ggcactacca gtgcaaaatg
      961 aagatgaaga gtatgacgaa gaggactatg aaagagagaa agagttgcag cagttactca
     1021 cagaccttcc ccatgacatg ctggatgacg acctctcctc tccagagctc cagtattcgg
     1081 actgcagcga ggatggcaca gacggacaac cacatcatcc tgagcaattg gagatgagct
     1141 ggaatgagca aatgctgccc aaatctcaaa gtgtaaatgg ctataatgaa attcagagtt
     1201 tatatgctgg agaaaaatgt ggtaatgtct gggaagaaaa tagaagtaaa actgaagacc
     1261 gacatcctgt gtaccatcct gaagaaggtg gagatgaagg tggaagtggt tatagtcctc
     1321 caagtaaatg tgaacagact gatttatatc accttcctga aaactttagg ccatatacca
     1381 atggtcagaa gcaggaattt aataaccaag caaccaatgt aattaaattt tcagatcctc
     1441 aatggaacca ttttcagggt cccagttgtc aaggtttgga accgtataat aaagtgacat
     1501 ataaacctta tcagtcttct gcccagaata atggctcacc agcccaggag ataacaggaa
     1561 gtgacacatt cgaaggcctg caacaacaat ttttaggagc taatgagaac tctgcagaaa
     1621 atatgcagat tattcaactt caggttctta acaaagcaaa agagagacaa ctggagaact
     1681 taattgaaaa gttaaatgaa agtgaacgtc aaattcgata tctgaatcac cagcttgtaa
     1741 taataaaaga tgaaaaggat ggtttgactc tcagccttcg agaatcacag aaactctttc
     1801 agaatggaaa agaaagagag atacagcttg aagctcaaat aaaagcactg gagactcaga
     1861 tacaagcatt aaaagtcaat gaagaacaga tgatcaagaa gtccagaaca actgaaatgg
     1921 ctctggaaag cttgaagcag cagctggtgg accttcatca ttctgaatca cttcaacgag
     1981 ctagagaaca gcatgagagc attgttatgg gcctcacaaa gaagtacgaa gagcaagtat
     2041 tgtccttaca aaagaatttg gatgccacag tcaccgcact taaagaacag gaagacattt
     2101 gctctcgtct gaaagatcac gtgaaacaac tggaaaggaa tcaagaagca atcaagttag
     2161 aaaagactga gatcattaat aagttgacaa gaagtctaga ggagagtcaa aagcagtgtg
     2221 cccacttgtt gcagtccggg tcagtacaag aggtggctca gctacagttc cagctgcagc
     2281 aagcacagaa ggcacatgct atgagtgcaa acatgaacaa ggctttgcaa gaagaattaa
     2341 cagaactaaa agatgaaatt tctctctatg aatctgctgc aaaactagga atacatccaa
     2401 gtgactcaga aggagaatta aatatagaac tcactgaatc gtatgtggat ttgggtatta
     2461 aaaaggtcaa ctggaaaaaa tccaaagtta ccagcattgt acaagaagaa gacccaaatg
     2521 aagagctttc aaaagatgag ttcattctga agttaaaggc agaagtacag cgtttgctgg
     2581 gtagcaactc aatgaagcgt catctggtgt ctcagttaca aaatgacctc aaagactgtc
     2641 ataagaaaat tgaagatctc caccaagtga agaaggatga aaaaagcatt gaggttgaga
     2701 ctaaaacaga tacctcagaa aaaccaaaga atcaattatg gcctgagtct tctacttctg
     2761 atgttgtcag agatgatatt ctgctgctta aaaatgaaat tcaagtttta caacaacaaa
     2821 atcaggaact taaagaaact gaaggaaaac tgagaaatac aaatcaagac ttatgtaatc
     2881 aaatgagaca aatggtacaa gattttgacc atgacaaaca agaagctgtg gataggtgtg
     2941 aaaggactta tcagcagcac catgaagcca tgaaaactca aatacgtgaa agcctattag
     3001 caaagcatgc tttggagaag cagcagctct ttgaggctta tgagagaact catttgcaac
     3061 tgaggtctga gttggataag ttgaataagg aggtgactgc tgtgcaggaa tgttacctag
     3121 aagtgtgcag agagaaggat aatctagaat tgactctcag gaagaccact gaaaaggagc
     3181 aacagactca ggagaagatc aaagaaaaac tcattcaaca gcttgaaaag gagtggcagt
     3241 ctaagctgga tcaaactata aaggcaatga aaaagaagac cttagattgt ggcagccaaa
     3301 ctgaccaagt aaccaccagt gatgttattt ccaagaaaga gatggcaatt atgatagaag
     3361 agcagaagtg cacaatccag caaaacttag aacaagagaa ggacatagcc atcaaggggg
     3421 ctatgaagaa actcgaaatt gaattggaac tcaaacattg tgaaaatatt accaaacagg
     3481 tagaaatagc tgtgcaaaat gctcatcagc gatggctggg agaactacca gagctggcag
     3541 agtatcaagc acttgtgaag gcagaacaga aaaagtggga agaacagcat gaggtctctg
     3601 tgaacaaaag gatatcattt gctgtttctg aagctaaaga gaaatggaag agtgagcttg
     3661 aaaatatgag gaaaaatata cttcctggaa aggaattgga agagaagatt cattctcttc
     3721 agaaggaact tgagttaaag aacgaagaag tccctgtggt catcagggct gagttagcta
     3781 aggctcggag tgaatggaac aaagaaaagc aagaagaaat ccacagaatc caagaacaaa
     3841 atgagcaaga ttaccggcaa tttttagatg atcaccgaaa taaaattaat gaggtgcttg
     3901 cggcagctaa agaagacttt atgaaacaaa aaactgaact acttcttcag aaggagacag
     3961 aattacaaac ttgtctagac cagagtcgta gagaatggac tatgcaggaa gccaagcgga
     4021 tccaactgga aatctatcag tatgaggaag acatcctgac tgtacttggg gttcttttaa
     4081 gtgataccca aaaggagcac atcagtgatt ctgaggacaa gcagcttttg gaaatcatgt
     4141 cgacttgttc ttcaaaatgg atgtctgtgc aatattttga aaaactaaag ggctgcatac
     4201 agaaagcatt tcaagataca cttcctctgc ttgtagaaaa cgctgaccca gaatggaaaa
     4261 agagaaatat ggccgagctc tctaaggatt ctgccagcca gggcactggc caaggagacc
     4321 ctggacctgc tgctggacac catgctcagc ccttggcctt acaagcaaca gaagcagaag
     4381 ctgataagaa aaaggtcctt gaaattaagg atttatgctg tggacactgc ttccaagaac
     4441 ttgaaaaggc aaagcaggaa tgtcaagatc tgaaaggaaa actggagaaa tgctgtaggc
     4501 atcttcagca tttagaaagg aagcacaaag ctgtagtgga aaaaattgga gaagagaata
     4561 ataaagttgt tgaagaatta atagaagaaa acaacgacat gaagaataaa ttggaagaat
     4621 tgcaaacact ttgtaaaaca ccaccaaggt cattgtcagc aggggccatt gaaaatgctt
     4681 gcctgccatg cagtggggga gccttggaag aacttcgtgg gcagtacatt aaagctgtaa
     4741 aaaaaattaa atgtgacatg cttcgttata ttcaggagag taaggaacga gctgcagaaa
     4801 tggtaaaagc agaggtactg cgagaacgtc aagaaaccgc ccgaaagatg cgcaaatatt
     4861 atttgatttg cctccaacag attttgcagg atgatggaaa agaaggggct gagaaaaaga
     4921 ttatgaatgc tgctagcaaa cttgctacaa tggcaaaatt actggaaaca cctatttcta
     4981 gtaagtccca aagcaaaact acacagtcag cactgcccct aacttcagag atgctgattg
     5041 cagttaaaaa atcaaaaaga aatgatgtga atcagaaaat accatgttgt attgaaagca
     5101 aatcaaatag tgtaaacacc atcaccagaa ctctgtgcga acaagctccc aagaggaggg
     5161 cagcttgtaa cttacaaagg ctgttagaga actcagagca tcagagcata aagcatgtgg
     5221 gatccaaaga gacacatttg gaattccagt ttggggatgg tagttgcaag cacctaaaca
     5281 gtttgccaag gaatgtttct cctgagtttg ttccttgtga aggtgaagga ggctttggtt
     5341 tgcacaagaa gaaagaccta ctcagtgata atggttctga atcacttccg cattcagctg
     5401 catacccctt tcttggaacc ttaggaaata aaccctcacc tagatgtacc cctggtcctt
     5461 ctgaatcagg atgcatgcat ataacctttc gcgattctaa tgaaagactt ggtttaaaag
     5521 tatataaatg caatccacta atggaaagtg aaaatgctgc atctgagaaa agtcaaggtt
     5581 tggatgttca ggaacctcca gtaaaagatg gaggggacct tagtgactgc ttgggctggc
     5641 cttccagcag tgcaacctta tcctttgaca gtcgtgaagc atcatttgta catggtaggc
     5701 cacaaggaac tttggaaata ccaagtgaat ctgttaaatc caaacagttt tcaccatccg
     5761 gttatctttc agatacagag gaaagtaata tgatttgtca aacaatgaaa tgtcagcgtt
     5821 atcaaactcc atacctgtca gaagaaacca cgtatttgga gccaggaaag atcagtgtga
     5881 attgtggaca cccatctcgt cataaggctg atagattaaa gtcagatttc aaaaaactga
     5941 gcagtacatt accatcttca gtgtgtcagc agccttcaag aaaattaatt gttccgctat
     6001 ctagccaaca agatagtggc tttgatagcc catttgttaa tctagactaa aaaaaaaaaa
     6061 aaaaaaaaaa aaaaaaaaaa ccgatatcca gcacagtggc ggccgctcga gtctagaggg
     6121 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6841 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7201 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     7261 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7321 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7381 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7441 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7501 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7561 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7621 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7681 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7741 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7801 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7861 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7921 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7981 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8041 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8101 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8161 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8221 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8341 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9301 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     9361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    10561 agatctcccg atcccctatg gt
//
