LOCUS       V023983                10177 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023983
VERSION     V023983
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4923
                     /label="CNTNAP2(NM_014141)"
                     /note="CNTNAP2(NM_014141)"
                     /gene="CNTNAP2"
     CDS             4939..4995
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4996..5706
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    5744..5968
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6014..6442
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6456..6785
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6852..7646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7820..7941
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7990..8006)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8014..8030
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8038..8068)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8083..8104
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8392..8977)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9148..10008)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10009..10113)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgcaggcgg ctccgcgcgc cggctgcggg
      961 gcagcgctcc tgctgtggat tgtcagcagc tgcctctgca gagcctggac ggctccctcc
     1021 acgtcccaaa aatgtgatga gccacttgtc tctggactcc cccatgtggc tttcagcagc
     1081 tcctcctcca tctctggtag ctattctccc ggctatgcca agataaacaa gagaggaggt
     1141 gctgggggat ggtctccatc agacagcgac cattatcaat ggcttcaggt tgactttggc
     1201 aatcggaagc agatcagtgc cattgcaacc caaggaaggt atagcagctc agattgggtg
     1261 acccaatacc ggatgctcta cagcgacaca gggagaaact ggaaacccta tcatcaagat
     1321 gggaatatct gggcatttcc cggaaacatt aactctgacg gtgtggtccg gcacgaatta
     1381 cagcatccga ttattgcccg ctatgtgcgc atagtgcctc tggattggaa tggagaaggt
     1441 cgcattggac tcagaattga agtttatggc tgttcttact gggctgatgt tatcaacttt
     1501 gatggccatg ttgtattacc atatagattc agaaacaaga agatgaaaac actgaaagat
     1561 gtcattgcct tgaactttaa gacgtctgaa agtgaaggag taatcctgca cggagaagga
     1621 cagcaaggag attacattac cttggaactg aaaaaagcca agctggtcct cagtttaaac
     1681 ttaggaagca accagcttgg ccccatatat ggccacacat cagtgatgac aggaagtttg
     1741 ctggatgacc accactggca ctctgtggtc attgagcgcc aggggcggag cattaacctc
     1801 actctggaca ggagcatgca gcacttccgt accaatggag agtttgacta cctggacttg
     1861 gactatgaga taacctttgg aggcatccct ttctctggca agcccagctc cagcagtaga
     1921 aagaatttca aaggctgcat ggaaagcatc aactacaatg gcgtcaacat tactgatctt
     1981 gccagaagga agaaattaga gccctcaaat gtgggaaatt tgagcttttc ttgtgtggaa
     2041 ccctatacgg tgcctgtctt tttcaacgct acaagttacc tggaggtgcc cggacggctt
     2101 aaccaggacc tgttctcagt cagtttccag tttaggacat ggaaccccaa tggtctcctg
     2161 gtcttcagtc actttgcgga taatttgggc aatgtggaga ttgacctcac tgaaagcaaa
     2221 gtgggtgttc acatcaacat cacacagacc aagatgagcc aaatcgatat ttcctcaggt
     2281 tctgggttga atgatggaca gtggcacgag gttcgcttcc tagccaagga aaattttgct
     2341 attctcacca tcgatggaga tgaagcatca gcagttcgaa ctaatagtcc ccttcaagtt
     2401 aaaactggcg agaagtactt ttttggaggt tttctgaacc agatgaataa ctcaagtcac
     2461 tctgtccttc agccttcatt ccaaggatgc atgcagctca ttcaagtgga cgatcaactt
     2521 gtaaatttat acgaagtggc acaaaggaag ccgggaagtt tcgcgaatgt cagcattgac
     2581 atgtgtgcga tcatagacag atgtgtgccc aatcactgtg agcatggtgg aaagtgctcg
     2641 caaacatggg acagcttcaa atgcacttgt gatgagacag gatacagtgg ggccacctgc
     2701 cacaactcta tctacgagcc ttcctgtgaa gcctacaaac acctaggaca gacatcaaat
     2761 tattactgga tagatcctga tggcagcgga cctctggggc ctctgaaagt ttactgcaac
     2821 atgacagagg acaaagtgtg gaccatagtg tctcatgact tgcagatgca gacgcctgtg
     2881 gtcggctaca acccagaaaa atactcagtg acacagctcg tttacagcgc ctccatggac
     2941 cagataagtg ccatcactga cagtgccgag tactgcgagc agtatgtctc ctatttctgc
     3001 aagatgtcaa gattgttgaa caccccagat ggaagccctt acacttggtg ggttggcaaa
     3061 gccaacgaga agcactacta ctggggaggc tctgggcctg gaatccagaa atgtgcctgc
     3121 ggcatcgaac gcaactgcac agatcccaag tactactgta actgcgacgc ggactacaag
     3181 caatggagga aggatgctgg tttcttatca tacaaagatc acctgccagt gagccaagtg
     3241 gtggttggag atactgaccg tcaaggctca gaagccaaat tgagcgtagg tcctctgcgc
     3301 tgccaaggag acaggaatta ttggaatgcc gcctctttcc caaacccatc ctcctacctg
     3361 cacttctcta ctttccaagg ggaaactagc gctgacattt ctttctactt caaaacatta
     3421 accccctggg gagtgtttct tgaaaatatg ggaaaggaag atttcatcaa gctggagctg
     3481 aagtctgcca cagaagtgtc cttttcattt gatgtgggaa atgggccagt agagattgta
     3541 gtgaggtcac caacccctct caacgatgac cagtggcacc gggtcactgc agagaggaat
     3601 gtcaagcagg ccagcctaca ggtggaccgg ctaccgcagc agatccgcaa ggccccaaca
     3661 gaaggccaca cccgcctgga gctctacagc cagttatttg tgggtggtgc tgggggccag
     3721 cagggcttcc tgggctgcat ccgctccttg aggatgaatg gggtgacact tgacctggag
     3781 gaaagagcaa aggtcacatc tgggttcata tccggatgct cgggccattg caccagctat
     3841 ggaacaaact gtgaaaatgg aggcaaatgc ctagagagat accacggtta ctcctgcgat
     3901 tgctctaata ctgcatatga tggaacattt tgcaacaaag atgttggtgc attttttgaa
     3961 gaagggatgt ggctacgata taactttcag gcaccagcaa caaatgccag agactccagc
     4021 agcagagtag acaacgctcc cgaccagcag aactcccacc cggacctggc acaggaggag
     4081 atccgcttca gcttcagcac caccaaggcg ccctgcattc tcctctacat cagctccttc
     4141 accacagact tcttggcagt cctcgtcaaa cccactggaa gcttacagat tcgatacaac
     4201 ctgggtggca cccgagagcc atacaatatt gacgtagacc acaggaacat ggccaatgga
     4261 cagccccaca gtgtcaacat cacccgccac gagaagacca tctttctcaa gctcgatcat
     4321 tatccttctg tgagttacca tctgccaagt tcatccgaca ccctcttcaa ttctcccaag
     4381 tcgctctttc tgggaaaagt tatagaaaca gggaaaattg accaagagat tcacaaatac
     4441 aacaccccag gattcactgg ttgcctctcc agagtccagt tcaaccagat cgcccctctc
     4501 aaggccgcct tgaggcagac aaacgcctcg gctcacgtcc acatccaggg cgagctggtg
     4561 gagtccaact gcggggcctc gccgctgacc ctctccccca tgtcgtccgc caccgacccc
     4621 tggcacctgg atcacctgga ttcagccagt gcggattttc catataatcc aggacaaggc
     4681 caagctataa gaaatggagt caacagaaac tcggctatca ttggaggcgt cattgctgtg
     4741 gtgattttca ccatcctgtg caccctggtc ttcctgatcc ggtacatgtt ccgccacaag
     4801 ggcacctacc ataccaacga agcaaagggg gcggagtcgg cagagagcgc ggacgccgcc
     4861 atcatgaaca acgaccccaa cttcacagag accattgatg aaagcaaaaa ggaatggctc
     4921 attctcgagg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag
     4981 gagaaccctg gacctatggt gagcaagggc gaggaggata acatggccat catcaaggag
     5041 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5101 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5161 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5221 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     5281 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     5341 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     5401 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     5461 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     5521 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5581 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     5641 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     5701 aagtaatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5761 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5821 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5881 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5941 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     6001 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6061 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6121 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6181 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6241 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6301 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6361 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6421 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6481 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6601 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6661 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6721 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6781 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     6841 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     6901 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     6961 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7021 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     7081 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     7141 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     7201 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     7261 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     7321 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     7381 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     7441 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     7501 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     7561 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     7621 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     7681 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7741 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7801 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7921 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7981 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8041 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8101 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8161 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8821 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     8941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10141 cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
