LOCUS       V021788                 8243 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021788
VERSION     V021788
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3682
                     /label="CORO7(NM_024535)"
                     /note="CORO7(NM_024535)"
                     /gene="CORO7"
     CDS             3689..3715
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3719..3745
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3749..3775
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3810..4034
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4080..4508
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4522..4851
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4918..5712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5886..6007
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6056..6072)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6080..6096
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6104..6134)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6149..6170
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6458..7043)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7214..8074)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8075..8179)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aaccgcttca gggtgtccaa gttccggcac accgaggctc ggccgccccg
      961 ccgcgagtcc tggatcagtg acattcgagc aggaaccgcc ccttcatgca ggaaccacat
     1021 caaatcaagc tgcagcttga tcgccttcaa ctccgaccgt cctggtgtac tgggcattgt
     1081 gcctctgcaa ggccaaggag aggacaagcg acgcgtggcc cacctgggct gccattcaga
     1141 cctagtcacc gacttggact tctcgccctt tgatgacttc ctcctggcca caggctcggc
     1201 tgacaggacg gtaaaactct ggcgactgcc agggcctggc caggccctgc cctcagcacc
     1261 cggggtggtg ctgggccccg aggacctccc agtggaggta ctgcagttcc accccacctc
     1321 tgacggcatt ctggtgagcg cagcaggcac cactgtgaag gtctgggacg cagccaagca
     1381 gcagcccctg acagagctgg cagcccatgg ggacctggtg cagagcgccg tctggagccg
     1441 agatggagcc ctggtgggca cggcgtgcaa ggacaagcag ctgcggatct ttgaccccag
     1501 aacaaagccg cgggcctctc agagcacgca ggcccatgag aacagcaggg atagccggct
     1561 ggcatggatg ggcacctggg agcaccttgt gtctactgga ttcaaccaga tgcgtgagcg
     1621 cgaagtgaag ctgtgggaca cgcggttctt ctccagcgcc ctggcctccc tcaccttgga
     1681 cacctcgctt gggtgtctcg tgcctctgct ggaccctgac tctgggctcc tggtcctggc
     1741 aggaaagggc gagaggcagc tgtactgtta cgaggtggtc ccgcagcagc cggcgctgag
     1801 cccagtgacc cagtgtgtcc tggagagcgt gctgcgtggg gctgcccttg tgccccggca
     1861 ggcgctggcc gtcatgagct gcgaggtact ccgcgtccta cagctgagcg acacagccat
     1921 cgtgcccatc ggctaccatg tgccccgcaa ggctgtggag ttccacgagg acctgttccc
     1981 ggacactgcc ggctgtgtgc ctgccaccga cccccatagc tggtgggctg gggacaacca
     2041 gcaggtgcag aaggtcagcc tcaaccccgc ctgccggccc cacccgagct tcacttcctg
     2101 tctggtgccc cctgcggagc ccctccctga cacagcccag cctgcggtga tggagacacc
     2161 cgtgggtgat gcagacgcaa gcgagggttt ctcttcccct cccagttcgc tgacctcgcc
     2221 ctccacgccc tccagcctgg ggccctcact ctccagcacc agtggcatcg ggaccagccc
     2281 cagtttgagg tcgctgcaga gcctgctggg ccccagttcc aagttccgcc atgctcaggg
     2341 cactgtcctg caccgagaca gccacatcac caacctcaag gggctcaacc tcaccacacc
     2401 tggtgagagt gacggcttct gtgccaacaa gctgcgtgtg gccgtgccgc tgctcagcag
     2461 cgggggacag gtggctgtgc ttgagctacg gaagcctggc cgcctgcccg acacggcact
     2521 gcccacgctg cagaatgggg cagctgtgac tgatctggcc tgggacccct ttgaccccca
     2581 tcgcctcgct gtggctggtg aggacgccag gatccgactg tggcgggtac ccgcagaggg
     2641 cctggaagag gtgctcacca cgccagagac tgtgctcaca ggccacacgg agaagatctg
     2701 ctccctgcgc ttccacccac tggcagccaa tgtgctggcc tcgtcctcct atgacctcac
     2761 tgttcgcatc tgggaccttc aggctggagc tgatcggctg aagctgcagg gccaccaaga
     2821 ccagatcttc agcctggcct ggagtcctga tgggcagcag ctggccactg tctgcaagga
     2881 tgggcgtgtg cgggtctaca ggccccggag tggccctgag cccctgcagg aaggcccagg
     2941 gcccaaggga ggacgcggag ctcgcattgt ctgggtatgt gatggtcgct gtctgctggt
     3001 gtctggcttt gacagccaaa gtgagcgcca gctgctccta tatgaagctg aggccctggc
     3061 cggcggaccc ttggcagtgt tgggcctgga cgtggctccc tcaaccctgc tgcccagcta
     3121 cgacccagac actggcctgg tgctcctgac cggcaagggc gacacccgtg tattcctgta
     3181 cgagctgctc cccgagtccc ctttcttcct ggagtgcaac agcttcacgt cgcctgaccc
     3241 ccacaagggc ctcgtcctcc tgcctaagac ggagtgcgac gtgcgggaag tggagctgat
     3301 gcggtgcctg cggctgcgtc agtcctccct ggagcctgtg gccttccggc tgccccgagt
     3361 ccggaaagag ttcttccagg atgacgtgtt cccagacacg gctgtgatct gggagcctgt
     3421 gctcagtgcc gaggcctggc tgcaaggcgc taatgggcag ccctggcttc tcagcctgca
     3481 gcctcctgac atgagcccag tgagccaagc cccccgagag gcccctgctc gtcgggcccc
     3541 atcctcagcg cagtacctgg aagaaaagtc tgaccagcaa aagaaggagg agctgctgaa
     3601 tgccatggtg gcaaaactgg ggaaccggga ggacccactc ccccaggact cctttgaagg
     3661 cgtggacgag gacgagtggg acctcgagta cccatacgac gtacctgatt atgcaactta
     3721 tccttatgat gtacctgatt atgctagcta tccttatgat gtcccagact acgcatgagg
     3781 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3961 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4021 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4501 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4681 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4741 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4801 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4861 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4921 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4981 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5041 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5101 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5161 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5221 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5281 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5341 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5401 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5461 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5521 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5581 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5641 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5701 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5761 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5821 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5881 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6001 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6061 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6961 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8221 gagatctccc gatcccctat ggt
//
