LOCUS V022637 5825 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022637 VERSION V022637 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..1315 /label="CXCL9(NM_002416)" /note="CXCL9(NM_002416)" /gene="CXCL9" CDS 1322..1387 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1425..1649 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1695..2123 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2137..2466 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2533..3327 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3501..3622 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3671..3687) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3695..3711 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3719..3749) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3764..3785 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4073..4658) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4829..5689) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5690..5794) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaagaaaa gtggtgttct 961 tttcctcttg ggcatcatct tgctggttct gattggagtg caaggaaccc cagtagtgag 1021 aaagggtcgc tgttcctgca tcagcaccaa ccaagggact atccacctac aatccttgaa 1081 agaccttaaa caatttgccc caagcccttc ctgcgagaaa attgaaatca ttgctacact 1141 gaagaatgga gttcaaacat gtctaaaccc agattcagca gatgtgaagg aactgattaa 1201 aaagtgggag aaacaggtca gccaaaagaa aaagcaaaag aatgggaaaa aacatcaaaa 1261 aaagaaagtt ctgaaagttc gaaaatctca acgttctcgt caaaagaaga ctacactcga 1321 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga 1381 tgacaagtag tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 1441 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 1501 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 1561 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 1621 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 1681 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 1741 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 1801 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 1861 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 1921 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 1981 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 2041 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 2101 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 2161 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 2221 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 2281 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 2341 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 2401 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 2461 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 2521 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 2581 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 2641 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 2701 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 2761 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 2821 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 2881 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 2941 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 3001 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 3061 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 3121 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 3181 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 3241 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 3301 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 3361 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 3421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 3481 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 3541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3601 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 3661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 3721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 3781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 3841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 3901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 4621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5821 acgtc //