LOCUS       V039420                 7335 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039420
VERSION     V039420
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             966..2807
                     /label="DDX5(NM_001320595)"
                     /note="DDX5(NM_001320595)"
                     /gene="DDX5"
     CDS             2820..2849
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2865..2882
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    2932..3156
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3202..3630
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3644..3973
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4040..4834
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5008..5129
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5178..5194)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5202..5218
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5226..5256)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5271..5292
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5580..6168)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6339..7199)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7200..7304)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcg
      961 ccaccatgtc gggttattcg agtgaccgag accgcggccg ggaccgaggg tttggtgcac
     1021 ctcgatttgg aggaagtagg gcagggccct tatctggaaa gaagtttgga aaccctgggg
     1081 agaaattagt taaaaagaag tggaatcttg atgagctgcc taaatttgag aagaattttt
     1141 atcaagagca ccctgatttg gctaggcgca cagcacaaga ggtggaaaca tacagaagaa
     1201 gcaaggaaat tacagttaga ggtcacaact gcccgaagcc agttctaaat ttttatgaag
     1261 ccaatttccc tgcaaatgtc atggatgtta ttgcaagaca gaatttcact gaacccactg
     1321 ctattcaagc tcagggatgg ccagttgctc taagtggatt ggatatggtt ggagtggcac
     1381 agactggatc tgggaaaaca ttgtcttatt tgcttcctgc cattgtccac atcaatcatc
     1441 agccattcct agagagaggc gatgggccta tttgtttggt gctggcacca actcgggaac
     1501 tggcccaaca ggtgcagcaa gtagctgctg aatattgtag agcatgtcgc ttgaagtcta
     1561 cttgtatcta cggtggtgct cctaagggac cacaaatacg tgatttggag agaggtgtgg
     1621 aaatctgtat tgcaacacct ggaagactga ttgacttttt agagtgtgga aaaaccaatc
     1681 tgagaagaac aacctacctt gtccttgatg aagcagatag aatgcttgat atgggctttg
     1741 aaccccaaat aaggaagatt gtggatcaaa taagacctga taggcaaact ctaatgtgga
     1801 gtgcgacttg gccaaaagaa gtaagacagc ttgctgaaga tttcctgaaa gactatattc
     1861 atataaacat tggtgcactt gaactgagtg caaaccacaa cattcttcag attgtggatg
     1921 tgtgtcatga cgtagaaaag gatgaaaaac ttattcgtct aatggaagag atcatgagtg
     1981 agaaggagaa taaaaccatt gtttttgtgg aaaccaaaag aagatgtgat gagcttacca
     2041 gaaaaatgag gagagatggg tggcctgcca tgggtatcca tggtgacaag agtcaacaag
     2101 agcgtgactg ggttctaaat gaattcaaac atggaaaagc tcctattctg attgctacag
     2161 atgtggcctc cagagggcta gatgtggaag atgtgaaatt tgtcatcaat tatgactacc
     2221 ctaactcctc agaggattat attcatcgaa ttggaagaac tgctcgcagt accaaaacag
     2281 gcacagcata cactttcttt acacctaata acataaagca agtgagcgac cttatctctg
     2341 tgcttcgtga agctaatcaa gcaattaatc ccaagttgct tcagttggtc gaagacagag
     2401 gttcaggtcg ttccaggggt agaggaggca tgaaggatga ccgtcgggac agatactctg
     2461 cgggcaaaag gggtggattt aataccttta gagacaggga aaattatgac agaggttact
     2521 ctagcctgct taaaagagat tttggggcaa aaactcagaa tggtgtttac agtgctgcaa
     2581 attacaccaa tgggagcttt ggaagtaatt ttgtgtctgc tggtatacag accagtttta
     2641 ggactggtaa tccaacaggg acttaccaga atggttatga tagcactcag caatacggaa
     2701 gtaatgttcc aaatatgcac aatggtatga accaacaggc atatgcatat cctgctactg
     2761 cagctgcacc tatgattggt tatccaatgc caacaggata ttcccaaaag cttgggcccg
     2821 aacaaaaact catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc
     2881 attgagttta aacggtctcc agcttaagtt taaaccgctg atcagcctcg actgtgcctt
     2941 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3001 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3061 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3121 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3181 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3241 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3301 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3361 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3421 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3481 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3541 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3601 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3721 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3781 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3841 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3901 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3961 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4021 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4081 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4141 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4201 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4261 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4321 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4381 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4441 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4501 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4561 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4621 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4681 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4741 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4801 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4861 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4921 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4981 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5041 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5101 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5161 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7321 gtgccacctg acgtc
//
