LOCUS V047023 8205 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047023 VERSION V047023 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 927..3011 /label="DVL1(NM_001330311)" /note="DVL1(NM_001330311)" /gene="DVL1" CDS 3048..3767 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3805..4029 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4075..4503 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4517..4846 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4913..5707 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5881..6002 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6051..6067) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6075..6091 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6099..6129) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6144..6165 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6453..7038) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7209..8069) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8070..8174) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccatgg cggagaccaa gattatctac cacatggacg 961 aggaggagac gccgtacctg gtcaagctgc ccgtggcccc cgagcgcgtc acgctggccg 1021 acttcaagaa cgtgctcagc aaccggcccg tgcacgccta caaattcttc tttaagtcca 1081 tggaccagga cttcggggtg gtgaaggagg agatctttga tgacaatgcc aagcttccct 1141 gcttcaacgg ccgcgtggtc tcctggctgg tcctggctga gggtgctcac tcggatgcgg 1201 ggtcccaggg cacggacagc cacacagacc tgcccccgcc tcttgagcgg acaggcggca 1261 tcggggactc ccggcccccc tccttccacc caaatgtggc cagcagccgt gacgggatgg 1321 acaacgagac aggcacggag tccatggtca gtcaccggcg ggagcgtgcc cgacgccgga 1381 accgcgagga ggccgcccgg accaatgggc acccaagggg agaccgacgg cgggatgtgg 1441 ggctgccccc agacagcgcg tccaccgccc tcagcagcga gcttgagtcc agcagctttg 1501 tggactcgga cgaggatggc agcacgagca ggctcagcag ctccacggag cagagcacct 1561 catccagact catccggaag cacaaacgcc ggcggaggaa gcagcgcctt cggcaggcgg 1621 accgggcctc ctccttcagc agcataaccg actccaccat gtccctcaac atcgtcactg 1681 tcacgctcaa catggaaaga catcactttc tgggcatcag catcgtgggg cagagcaacg 1741 accgtggaga cggcggcatc tacattggct ccatcatgaa gggcggggct gtggccgctg 1801 acggccgcat cgagcccggc gacatgttgc tgcaggtgaa tgacgtgaac tttgagaaca 1861 tgagcaatga cgatgccgtg cgggtgctgc gggagatcgt ttcccagacg gggcccatca 1921 gcctcactgt ggccaagtgc tgggacccaa cgccccgaag ctacttcacc gtcccacggg 1981 ctgacccggt gcggcccatc gaccccgccg cctggctgtc ccacacggcg gcactgacag 2041 gagccctgcc ccgctacggt acgagtccct gctccagcgc cgtcacgcgc accagctcct 2101 cctcactaac cagctccgtg cctggtgctc cacagctgga agaggcgccg ctgacggtga 2161 agagtgacat gagcgccgtc gtccgggtca tgcagctgcc agactcggga ctggagatcc 2221 gcgaccgcat gtggctcaag atcaccatcg ccaatgccgt catcggggcg gacgtggtgg 2281 actggctgta cacacacgtg gagggcttca aggagcggcg ggaggcccgg aagtacgcca 2341 gcagcttgct gaagcacggc ttcctgcggc acacggtcaa caagatcacc ttctccgagc 2401 agtgctacta cgtcttcggg gatctctgca gcaatctcgc caccctgaac ctcaacagtg 2461 gctccagtgg gacttcggat caggacacgc tggccccgct gccccacccg gctgccccct 2521 ggcctctggg tcagggctac ccctaccagt acccgggacc cccaccctgc ttcccgcctg 2581 cctaccagga cccgggcttt agctatggca gcggcagcac cgggagtcag cagagtgaag 2641 ggagcaaaag cagtgggtcc acccggagca gccgccgggc cccgggccgt gagaaggagc 2701 gtcgggcggc gggagctggg ggcagtggca gtgaatcgga tcacacggca ccgagtgggg 2761 tggggagcag ctggcgagag cgtccggccg gccagctcag ccgtggcagc agcccacgca 2821 gtcaggcctc ggctaccgcc ccggggctcc ccccgcccca ccccacgacc aaggcctata 2881 cagtggtggg ggggccaccc gggggacccc ctgtccggga gctggctgcc gtccccccgg 2941 aattgacagg cagccgccag tccttccaga aggctatggg gaacccctgc gagttcttcg 3001 tggacatcat gctcgagggc ggtggaggaa gcggcggagg tggatctatg gtgagcaagg 3061 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3241 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3301 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3481 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3541 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3601 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 3661 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3721 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagggcccg 3781 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3841 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3901 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3961 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 4021 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 4081 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4141 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4201 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4261 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4321 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4381 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4441 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4501 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4561 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4621 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4681 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4741 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4801 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4861 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4921 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4981 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 5041 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 5101 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 5161 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 5221 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 5281 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5341 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5401 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5461 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5521 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5581 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5641 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5701 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5761 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5821 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6001 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 6061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 6241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 6301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 6901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6961 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 7021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 7081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 8041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 8101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc //