LOCUS       V035085                 6823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035085
VERSION     V035085
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2316
                     /label="EEF1A1(NM_001402)"
                     /note="EEF1A1(NM_001402)"
                     /gene="EEF1A1"
     CDS             2323..2352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    2390..2614
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      2660..3088
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3102..3431
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3498..4292
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4466..4587
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4636..4652)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4660..4676
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4684..4714)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4729..4750
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5038..5623)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5794..6654)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6655..6759)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgggaaagg aaaagactca tatcaacatt
      961 gtcgtcattg gacacgtaga ttcgggcaag tccaccacta ctggccatct gatctataaa
     1021 tgcggtggca tcgacaaaag aaccattgaa aaatttgaga aggaggctgc tgagatggga
     1081 aagggctcct tcaagtatgc ctgggtcttg gataaactga aagctgagcg tgaacgtggt
     1141 atcaccattg atatctcctt gtggaaattt gagaccagca agtactatgt gactatcatt
     1201 gatgccccag gacacagaga ctttatcaaa aacatgatta cagggacatc tcaggctgac
     1261 tgtgctgtcc tgattgttgc tgctggtgtt ggtgaatttg aagctggtat ctccaagaat
     1321 gggcagaccc gagagcatgc ccttctggct tacacactgg gtgtgaaaca actaattgtc
     1381 ggtgttaaca aaatggattc cactgagcca ccctacagcc agaagagata tgaggaaatt
     1441 gttaaggaag tcagcactta cattaagaaa attggctaca accccgacac agtagcattt
     1501 gtgccaattt ctggttggaa tggtgacaac atgctggagc caagtgctaa catgccttgg
     1561 ttcaagggat ggaaagtcac ccgtaaggat ggcaatgcca gtggaaccac gctgcttgag
     1621 gctctggact gcatcctacc accaactcgt ccaactgaca agcccttgcg cctgcctctc
     1681 caggatgtct acaaaattgg tggtattggt actgttcctg ttggccgagt ggagactggt
     1741 gttctcaaac ccggtatggt ggtcaccttt gctccagtca acgttacaac ggaagtaaaa
     1801 tctgtcgaaa tgcaccatga agctttgagt gaagctcttc ctggggacaa tgtgggcttc
     1861 aatgtcaaga atgtgtctgt caaggatgtt cgtcgtggca acgttgctgg tgacagcaaa
     1921 aatgacccac caatggaagc agctggcttc actgctcagg tgattatcct gaaccatcca
     1981 ggccaaataa gcgccggcta tgcccctgta ttggattgcc acacggctca cattgcatgc
     2041 aagtttgctg agctgaagga aaagattgat cgccgttctg gtaaaaagct ggaagatggc
     2101 cctaaattct tgaagtctgg tgatgctgcc attgttgata tggttcctgg caagcccatg
     2161 tgtgttgaga gcttctcaga ctatccacct ttgggtcgct ttgctgttcg tgatatgaga
     2221 cagacagttg cggtgggtgt catcaaagca gtggacaaga aggctgctgg agctggcaag
     2281 gtcaccaagt ctgcccagaa agctcagaag gctaaactcg aggagcagaa gctgatctca
     2341 gaggaggacc tgtagtgagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2641 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3061 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3361 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3421 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     3481 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3541 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3601 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3661 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3721 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3781 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3841 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3901 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3961 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4021 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4081 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4141 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4201 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4261 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4321 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4381 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4441 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4561 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     5581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6781 acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
