LOCUS       V026197                 8489 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026197
VERSION     V026197
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..3979
                     /label="ELAPOR1(NM_020775)"
                     /note="ELAPOR1(NM_020775)"
                     /gene="ELAPOR1"
     CDS             3986..4051
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4089..4313
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4359..4787
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4801..5130
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5197..5991
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6165..6286
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6335..6351)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6359..6375
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6383..6413)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6428..6449
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6737..7322)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7493..8353)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8354..8458)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggctgagc ctgggcacag
      961 ccaccatctc tccgccagag tcaggggaag aactgagagg cgcatacccc ggctgtggcg
     1021 gctgctgctc tgggctggga ccgccttcca ggtgacccag ggaacgggac cggagcttca
     1081 tgcctgcaaa gagtctgagt accactatga gtacacggcg tgtgacagca cgggttccag
     1141 gtggagggtc gccgtgccgc ataccccggg cctgtgcacc agcctgcctg accccatcaa
     1201 gggcaccgag tgctccttct cctgcaacgc cggggagttt ctggatatga aggaccagtc
     1261 atgtaagcca tgcgctgagg gccgctactc cctcggcaca ggcattcggt ttgatgagtg
     1321 ggatgagctg ccccatggct ttgccagcct ctcagccaac atggagctgg atgacagtgc
     1381 tgctgagtcc accgggaact gtacttcgtc caagtgggtt ccccggggcg actacatcgc
     1441 ctccaacacg gacgaatgca cagccacact gatgtacgcc gtcaacctga agcaatctgg
     1501 caccgttaac ttcgaatact actatccaga ctccagcatc atctttgagt ttttcgttca
     1561 gaatgaccag tgccagccca atgcagatga ctccaggtgg atgaagacca cagagaaagg
     1621 atgggaattc cacagtgtgg agctaaatcg aggcaataat gtcctctatt ggagaaccac
     1681 agccttctca gtatggacca aagtacccaa gcctgtgctg gtgagaaaca ttgccataac
     1741 aggggtggcc tacacttcag aatgcttccc ctgcaaacct ggcacgtatg cagacaagca
     1801 gggctcctct ttctgcaaac tttgcccagc caactcttat tcaaataaag gagaaacttc
     1861 ttgccaccag tgtgaccctg acaaatactc agagaaagga tcttcttcct gtaacgtgcg
     1921 cccagcttgc acagacaaag attatttcta cacacacacg gcctgcgatg ccaacggaga
     1981 gacacaactc atgtacaaat gggccaagcc gaaaatctgt agcgaggacc ttgagggggc
     2041 agtgaagctg cctgcctctg gtgtgaagac ccactgccca ccctgcaacc caggcttctt
     2101 caaaaccaac aacagcacct gccagccctg cccatatggt tcctactcca atggctcaga
     2161 ctgtacccgc tgccctgcag ggactgaacc tgctgtggga tttgaataca aatggtggaa
     2221 cacgctgccc acaaacatgg aaacgaccgt tctcagtggg atcaacttcg agtacaaggg
     2281 catgacaggc tgggaggtgg ctggtgatca catttacaca gctgctggag cctcagacaa
     2341 tgacttcatg attctcactc tggttgtgcc aggatttaga cctccgcagt cggtgatggc
     2401 agacacagag aataaagagg tggccagaat cacatttgtc tttgagaccc tctgttctgt
     2461 gaactgtgag ctctacttca tggtgggtgt gaattctagg accaacactc ctgtggagac
     2521 gtggaaaggt tccaaaggca aacagtccta tacctacatc attgaggaga acactaccac
     2581 gagcttcacc tgggccttcc agaggaccac ttttcatgag gcaagcagga agtacaccaa
     2641 tgacgttgcc aagatctact ccatcaatgt caccaatgtt atgaatggtg tggcctccta
     2701 ctgccgtccc tgtgccctag aagcctctga tgtgggctcc tcctgcacct cttgtcctgc
     2761 tggttactat attgaccgag attcaggaac ctgccactcc tgccccacta acacaattct
     2821 gaaagcccac cagccttatg gtgtccaggc ctgtgtgccc tgtggtccag ggaccaagaa
     2881 caacaagatc cactctctgt gctacaacga ttgcaccttc tcacgcaaca ctccgaccag
     2941 gactttcaac tacaacttct ccgctttggc aaacactgtc actcttgctg gagggccaag
     3001 cttcacttcc aaagggctga aatacttcca tcactttacc ctcagtctct gtggaaacca
     3061 gggtaggaaa atgtctgtgt gcaccgacaa tgtcactgac ctccggattc ctgagggtga
     3121 gtcagggttc tccaaatcta tcacagccta cgtctgccag gcagtcatca tccccccaga
     3181 ggtgacaggc tacaaggccg gggtttcctc acagcctgtc agccttgctg atcgacttat
     3241 tggggtgaca acagatatga ctctggatgg aatcacctcc ccagctgaac ttttccacct
     3301 ggagtccttg ggaataccgg acgtgatctt cttttatagg tccaatgatg tgacccagtc
     3361 ctgcagttct gggagatcaa ccaccatccg cgtcaggtgc agtccacaga aaactgtccc
     3421 tggaagtttg ctgctgccag gaacgtgctc ggatgggacc tgtgatggct gcaacttcca
     3481 cttcctgtgg gagagcgcgg ctgcttgccc gctctgctca gtggctgact accatgctat
     3541 cgtcagcagc tgtgtggctg ggatccagaa gactacttac gtgtggcgag aacccaagct
     3601 atgctctggt ggcatttctc tgcctgagca gagagtcacc atctgcaaaa ccatagattt
     3661 ctggctgaaa gtgggcatct ctgcaggcac ctgtactgcc atcctgctca ccgtcttgac
     3721 ctgctacttt tggaaaaaga atcaaaaact agagtacaag tactccaagc tggtgatgaa
     3781 tgctactctc aaggactgtg acctgccagc agctgacagc tgcgccatca tggaaggcga
     3841 ggatgtagag gacgacctca tctttaccag caagaagtca ctctttggga agatcaaatc
     3901 atttacctcc aagaggactc ctgatggatt tgactcagtg ccgctgaaga catcctcagg
     3961 aggcctagac atggacctgc tcgaggacta caaagaccat gacggtgatt ataaagatca
     4021 tgacatcgac tacaaggatg acgatgacaa gtagtgaggg cccgtttaaa cccgctgatc
     4081 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4141 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4201 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4261 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4321 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4801 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5161 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5221 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5281 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5341 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5401 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5461 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5521 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5581 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5641 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5701 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5761 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5821 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5881 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5941 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6001 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6061 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6121 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6301 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6541 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7261 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8461 catttccccg aaaagtgcca cctgacgtc
//
