LOCUS       V033019                12658 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033019
VERSION     V033019
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             932..8173
                     /label="EP300(NM_001429)"
                     /note="EP300(NM_001429)"
                     /gene="EP300"
     CDS             8195..8224
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    8253..8477
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      8523..8951
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8965..9294
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9361..10155
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10331..10452
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10501..10517)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10525..10541
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10549..10579)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10594..10615
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10903..11491)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11662..12522)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12523..12627)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccgccac catggccgag aatgtggtgg aaccggggcc
      961 gccttcagcc aagcggccta aactctcatc tccggccctc tcggcgtccg ccagcgatgg
     1021 cacagatttt ggctctctat ttgacttgga gcacgactta ccagatgaat taatcaactc
     1081 tacagaattg ggactaacca atggtggtga tattaatcag cttcagacaa gtcttggcat
     1141 ggtacaagat gcagcttcta aacataaaca gctgtcagaa ttgctgcgat ctggtagttc
     1201 ccctaacctc aatatgggag ttggtggccc aggtcaagtc atggccagcc aggcccaaca
     1261 gagcagtcct ggattaggtt tgataaatag catggtcaaa agcccaatga cacaggcagg
     1321 cttgacttct cccaacatgg ggatgggcac tagtggacca aatcagggtc ctacgcagtc
     1381 aacaggtatg atgaacagtc cagtaaatca gcctgccatg ggaatgaaca cagggatgaa
     1441 tgcgggcatg aatcctggaa tgttggctgc aggcaatgga caagggataa tgcctaatca
     1501 agtcatgaac ggttcaattg gagcaggccg agggcgacag aatatgcagt acccaaaccc
     1561 aggcatggga agtgctggca acttactgac tgagcctctt cagcagggct ctccccagat
     1621 gggaggacaa acaggattga gaggccccca gcctcttaag atgggaatga tgaacaaccc
     1681 caatccttat ggttcaccat atactcagaa tcctggacag cagattggag ccagtggcct
     1741 tggtctccag attcagacaa aaactgtact atcaaataac ttatctccat ttgctatgga
     1801 caaaaaggca gttcctggtg gaggaatgcc caacatgggt caacagccag ccccgcaggt
     1861 ccagcagcca ggcctggtga ctccagttgc ccaagggatg ggttctggag cacatacagc
     1921 tgatccagag aagcgcaagc tcatccagca gcagcttgtt ctccttttgc atgctcacaa
     1981 gtgccagcgc cgggaacagg ccaatgggga agtgaggcag tgcaaccttc cccactgtcg
     2041 cacaatgaag aatgtcctaa accacatgac acactgccag tcaggcaagt cttgccaagt
     2101 ggcacactgt gcatcttctc gacaaatcat ttcacactgg aagaattgta caagacatga
     2161 ttgtcctgtg tgtctccccc tcaaaaatgc tggtgataag agaaatcaac agccaatttt
     2221 gactggagca cccgttggac ttggaaatcc tagctctcta ggggtgggtc aacagtctgc
     2281 ccccaaccta agcactgtta gtcagattga tcccagctcc atagaaagag cctatgcagc
     2341 tcttggacta ccctatcaag taaatcagat gccgacacaa ccccaggtgc aagcaaagaa
     2401 ccagcagaat cagcagcctg ggcagtctcc ccaaggcatg cggcccatga gcaacatgag
     2461 tgctagtcct atgggagtaa atggaggtgt aggagttcaa acgccgagtc ttctttctga
     2521 ctcaatgttg cattcagcca taaattctca aaacccaatg atgagtgaaa atgccagtgt
     2581 gccctccctg ggtcctatgc caacagcagc tcaaccatcc actactggaa ttcggaaaca
     2641 gtggcacgaa gatattactc aggatcttcg aaatcatctt gttcacaaac tcgtccaagc
     2701 catatttcct acgccggatc ctgctgcttt aaaagacaga cggatggaaa acctagttgc
     2761 atatgctcgg aaagttgaag gggacatgta tgaatctgca aacaatcgag cggaatacta
     2821 ccaccttcta gctgagaaaa tctataagat ccagaaagaa ctagaagaaa aacgaaggac
     2881 cagactacag aagcagaaca tgctaccaaa tgctgcaggc atggttccag tttccatgaa
     2941 tccagggcct aacatgggac agccgcaacc aggaatgact tctaatggcc ctctacctga
     3001 cccaagtatg atccgtggca gtgtgccaaa ccagatgatg cctcgaataa ctccacaatc
     3061 tggtttgaat caatttggcc agatgagcat ggcccagccc cctattgtac cccggcaaac
     3121 ccctcctctt cagcaccatg gacagttggc tcaacctgga gctctcaacc cgcctatggg
     3181 ctatgggcct cgtatgcaac agccttccaa ccagggccag ttccttcctc agactcagtt
     3241 cccatcacag ggaatgaatg taacaaatat ccctttggct ccgtccagcg gtcaagctcc
     3301 agtgtctcaa gcacaaatgt ctagttcttc ctgcccggtg aactctccta taatgcctcc
     3361 agggtctcag gggagccaca ttcactgtcc ccagcttcct caaccagctc ttcatcagaa
     3421 ttcaccctcg cctgtaccta gtcgtacccc cacccctcac catactcccc caagcatagg
     3481 ggctcagcag ccaccagcaa caacaattcc agcccctgtt cctacacctc ctgccatgcc
     3541 acctgggcca cagtcccagg ctctacatcc ccctccaagg cagacaccta caccaccaac
     3601 aacacaactt ccccaacaag tgcagccttc acttcctgct gcaccttctg ctgaccagcc
     3661 ccagcagcag cctcgctcac agcagagcac agcagcgtct gttcctaccc caacagcacc
     3721 gctgcttcct ccgcagcctg caactccact ttcccagcca gctgtaagca ttgaaggaca
     3781 ggtatcaaat cctccatcta ctagtagcac agaagtgaat tctcaggcca ttgctgagaa
     3841 gcagccttcc caggaagtga agatggaggc caaaatggaa gtggatcaac cagaaccagc
     3901 agatactcag ccggaggata tttcagagtc taaagtggaa gactgtaaaa tggaatctac
     3961 cgaaacagaa gagagaagca ctgagttaaa aactgaaata aaagaggagg aagaccagcc
     4021 aagtacttca gctacccagt catctccggc tccaggacag tcaaagaaaa agattttcaa
     4081 accagaagaa ctacgacagg cactgatgcc aactttggag gcactttacc gtcaggatcc
     4141 agaatccctt ccctttcgtc aacctgtgga ccctcagctt ttaggaatcc ctgattactt
     4201 tgatattgtg aagagcccca tggatctttc taccattaag aggaagttag acactggaca
     4261 gtatcaggag ccctggcagt atgtcgatga tatttggctt atgttcaata atgcctggtt
     4321 atataaccgg aaaacatcac gggtatacaa atactgctcc aagctctctg aggtctttga
     4381 acaagaaatt gacccagtga tgcaaagcct tggatactgt tgtggcagaa agttggagtt
     4441 ctctccacag acactgtgtt gctacggcaa acagttgtgc acaatacctc gtgatgccac
     4501 ttattacagt taccagaaca ggtatcattt ctgtgagaag tgtttcaatg agatccaagg
     4561 ggagagcgtt tctttggggg atgacccttc ccagcctcaa actacaataa ataaagaaca
     4621 attttccaag agaaaaaatg acacactgga tcctgaactg tttgttgaat gtacagagtg
     4681 cggaagaaag atgcatcaga tctgtgtcct tcaccatgag atcatctggc ctgctggatt
     4741 cgtctgtgat ggctgtttaa agaaaagtgc acgaactagg aaagaaaata agttttctgc
     4801 taaaaggttg ccatctacca gacttggcac ctttctagag aatcgtgtga atgactttct
     4861 gaggcgacag aatcaccctg agtcaggaga ggtcactgtt agagtagttc atgcttctga
     4921 caaaaccgtg gaagtaaaac caggcatgaa agcaaggttt gtggacagtg gagagatggc
     4981 agaatccttt ccataccgaa ccaaagccct ctttgccttt gaagaaattg atggtgttga
     5041 cctgtgcttc tttggcatgc atgttcaaga gtatggctct gactgccctc cacccaacca
     5101 gaggagagta tacatatctt acctcgatag tgttcatttc ttccgtccta aatgcttgag
     5161 gactgcagtc tatcatgaaa tcctaattgg atatttagaa tatgtcaaga aattaggtta
     5221 cacaacaggg catatttggg catgtccacc aagtgaggga gatgattata tcttccattg
     5281 ccatcctcct gaccagaaga tacccaagcc caagcgactg caggaatggt acaaaaaaat
     5341 gcttgacaag gctgtatcag agcgtattgt ccatgactac aaggatattt ttaaacaagc
     5401 tactgaagat agattaacaa gtgcaaagga attgccttat ttcgagggtg atttctggcc
     5461 caatgttctg gaagaaagca ttaaggaact ggaacaggag gaagaagaga gaaaacgaga
     5521 ggaaaacacc agcaatgaaa gcacagatgt gaccaaggga gacagcaaaa atgctaaaaa
     5581 gaagaataat aagaaaacca gcaaaaataa gagcagcctg agtaggggca acaagaagaa
     5641 acccgggatg cccaatgtat ctaacgacct ctcacagaaa ctatatgcca ccatggagaa
     5701 gcataaagag gtcttctttg tgatccgcct cattgctggc cctgctgcca actccctgcc
     5761 tcccattgtt gatcctgatc ctctcatccc ctgcgatctg atggatggtc gggatgcgtt
     5821 tctcacgctg gcaagggaca agcacctgga gttctcttca ctccgaagag cccagtggtc
     5881 caccatgtgc atgctggtgg agctgcacac gcagagccag gaccgctttg tctacacctg
     5941 caatgaatgc aagcaccatg tggagacacg ctggcactgt actgtctgtg aggattatga
     6001 cttgtgtatc acctgctata acactaaaaa ccatgaccac aaaatggaga aactaggcct
     6061 tggcttagat gatgagagca acaaccagca ggctgcagcc acccagagcc caggcgattc
     6121 tcgccgcctg agtatccagc gctgcatcca gtctctggtc catgcttgcc agtgtcggaa
     6181 tgccaattgc tcactgccat cctgccagaa gatgaagcgg gttgtgcagc ataccaaggg
     6241 ttgcaaacgg aaaaccaatg gcgggtgccc catctgcaag cagctcattg ccctctgctg
     6301 ctaccatgcc aagcactgcc aggagaacaa atgcccggtg ccgttctgcc taaacatcaa
     6361 gcagaagctc cggcagcaac agctgcagca ccgactacag caggcccaaa tgcttcgcag
     6421 gaggatggcc agcatgcagc ggactggtgt ggttgggcag caacagggcc tcccttcccc
     6481 cactcctgcc actccaacga caccaactgg ccaacagcca accaccccgc agacgcccca
     6541 gcccacttct cagcctcagc ctacccctcc caatagcatg ccaccctact tgcccaggac
     6601 tcaagctgct ggccctgtgt cccagggtaa ggcagcaggc caggtgaccc ctccaacccc
     6661 tcctcagact gctcagccac cccttccagg gcccccacct gcagcagtgg aaatggcaat
     6721 gcagattcag agagcagcgg agacgcagcg ccagatggcc cacgtgcaaa tttttcaaag
     6781 gccaatccaa caccagatgc ccccgatgac tcccatggcc cccatgggta tgaacccacc
     6841 tcccatgacc agaggtccca gtgggcattt ggagccaggg atgggaccga cagggatgca
     6901 gcaacagcca ccctggagcc aaggaggatt gcctcagccc cagcaactac agtctgggat
     6961 gccaaggcca gccatgatgt cagtggccca gcatggtcaa cctttgaaca tggctccaca
     7021 accaggattg ggccaggtag gtatcagccc actcaaacca ggcactgtgt ctcaacaagc
     7081 cttacaaaac cttttgcgga ctctcaggtc tcccagctct cccctgcagc agcaacaggt
     7141 gcttagtatc cttcacgcca acccccagct gttggctgca ttcatcaagc agcgggctgc
     7201 caagtatgcc aactctaatc cacaacccat ccctgggcag cctggcatgc cccaggggca
     7261 gccagggcta cagccaccta ccatgccagg tcagcagggg gtccactcca atccagccat
     7321 gcagaacatg aatccaatgc aggcgggcgt tcagagggct ggcctgcccc agcagcaacc
     7381 acagcagcaa ctccagccac ccatgggagg gatgagcccc caggctcagc agatgaacat
     7441 gaaccacaac accatgcctt cacaattccg agacatcttg agacgacagc aaatgatgca
     7501 acagcagcag caacagggag cagggccagg aataggccct ggaatggcca accataacca
     7561 gttccagcaa ccccaaggag ttggctaccc accacagcag cagcagcgga tgcagcatca
     7621 catgcaacag atgcaacaag gaaatatggg acagataggc cagcttcccc aggccttggg
     7681 agcagaggca ggtgccagtc tacaggccta tcagcagcga ctccttcagc aacagatggg
     7741 gtcccctgtt cagcccaacc ccatgagccc ccagcagcat atgctcccaa atcaggccca
     7801 gtccccacac ctacaaggcc agcagatccc taattctctc tccaatcaag tgcgctctcc
     7861 ccagcctgtc ccttctccac ggccacagtc ccagcccccc cactccagtc cttccccaag
     7921 gatgcagcct cagccttctc cacaccacgt ttccccacag acaagttccc cacatcctgg
     7981 actggtagct gcccaggcca accccatgga acaagggcat tttgccagcc cggaccagaa
     8041 ttcaatgctt tctcagcttg ctagcaatcc aggcatggca aacctccatg gtgcaagcgc
     8101 cacggacctg ggactcagca ccgataactc agacttgaat tcaaacctct cacagagtac
     8161 actagacata cacctcgagt ctagagggcc cttcgaacaa aaactcatct cagaagagga
     8221 tctgtgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     8281 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     8341 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     8401 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     8461 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     8521 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8581 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8641 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8701 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8761 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8821 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8881 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8941 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     9001 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     9061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9121 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     9241 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9301 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     9361 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     9421 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     9481 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     9541 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     9601 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     9661 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     9721 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     9781 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     9841 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     9901 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     9961 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    10021 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    10081 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    10141 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
    10201 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10261 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10321 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10441 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    10501 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10561 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10621 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10681 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10741 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10801 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10861 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10921 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10981 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11041 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11101 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11161 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11221 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11281 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11341 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11401 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11461 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11521 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11581 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11641 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11941 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12181 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12241 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12541 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12601 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
