LOCUS       V028907                 7783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028907
VERSION     V028907
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             950..1603
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1640..3304
                     /label="EPHX2(NM_001979)"
                     /note="EPHX2(NM_001979)"
                     /gene="EPHX2"
     promoter        complement(3335..3353)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     polyA_signal    3379..3603
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3649..4077
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4091..4421
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4488..5282
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5456..5577
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5626..5642)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5650..5666
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5674..5704)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5719..5740
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6028..6616)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6787..7647)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7648..7752)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgtcccctat
      961 actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct
     1021 tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa
     1081 aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa
     1141 attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg
     1201 ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata
     1261 cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag
     1321 caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa
     1381 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata
     1441 catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga
     1501 agctatccca caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca
     1561 gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaaggaggag ggggaagcgg
     1621 cggcggaggg agtgatatca tgacgctgcg cgcggccgtc ttcgaccttg acggggtgct
     1681 ggcgctgcca gcggtgttcg gcgtcctcgg ccgcacggag gaggccctgg cgctgcccag
     1741 aggacttctg aatgatgctt tccagaaagg gggaccagag ggtgccacta cccggcttat
     1801 gaaaggagag atcacacttt cccagtggat accactcatg gaagaaaact gcaggaagtg
     1861 ctccgagacc gctaaagtct gcctccccaa gaatttctcc ataaaagaaa tctttgacaa
     1921 ggcgatttca gccagaaaga tcaaccgccc catgctccag gcagctctca tgctcaggaa
     1981 gaaaggattc actactgcca tcctcaccaa cacctggctg gacgaccgtg ctgagagaga
     2041 tggcctggcc cagctgatgt gtgagctgaa gatgcacttt gacttcctga tagagtcgtg
     2101 tcaggtggga atggtcaaac ctgaacctca gatctacaag tttctgctgg acaccctgaa
     2161 ggccagcccc agtgaggtcg tttttttgga tgacatcggg gctaatctga agccagcccg
     2221 tgacttggga atggtcacca tcctggtcca ggacactgac acggccctga aagaactgga
     2281 gaaagtgacc ggaatccagc ttctcaatac cccggcccct ctgccgacct cttgcaatcc
     2341 aagtgacatg agccatgggt acgtgacagt aaagcccagg gtccgtctgc attttgtgga
     2401 gctgggctcc ggccctgctg tgtgcctctg ccatggattt cccgagagtt ggtattcttg
     2461 gaggtaccag atccctgctc tggcccaggc aggttaccgg gtcctagcta tggacatgaa
     2521 aggctatgga gagtcatctg ctcctcccga aatagaagaa tattgcatgg aagtgttatg
     2581 taaggagatg gtaaccttcc tggataaact gggcctctct caagcagtgt tcattggcca
     2641 tgactggggt ggcatgctgg tgtggtacat ggctctcttc taccccgaga gagtgagggc
     2701 ggtggccagt ttgaatactc ccttcatacc agcaaatccc aacatgtccc ctttggagag
     2761 tatcaaagcc aacccagtat ttgattacca gctctacttc caagaaccag gagtggctga
     2821 ggctgaactg gaacagaacc tgagtcggac tttcaaaagc ctcttcagag caagcgatga
     2881 gagtgtttta tccatgcata aagtctgtga agcgggagga ctttttgtaa atagcccaga
     2941 agagcccagc ctcagcagga tggtcactga ggaggaaatc cagttctatg tgcagcagtt
     3001 caagaagtct ggtttcagag gtcctctaaa ctggtaccga aacatggaaa ggaactggaa
     3061 gtgggcttgc aaaagcttgg gacggaagat cctgattccg gccctgatgg tcacggcgga
     3121 gaaggacttc gtgctcgttc ctcagatgtc ccagcacatg gaggactgga ttccccacct
     3181 gaaaagggga cacattgagg actgtgggca ctggacacag atggacaagc caaccgaggt
     3241 gaatcagatc ctcattaagt ggctggattc tgatgcccgg aacccaccgg tggtctcaaa
     3301 gatgtagctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     3361 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3421 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3481 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3541 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3601 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3781 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
     3841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
     4021 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4081 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
     4141 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4321 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4381 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4441 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4501 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4561 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4621 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     4681 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4741 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4801 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4861 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4921 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4981 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5041 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5101 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5161 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5221 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5281 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5341 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5401 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5581 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     5641 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5701 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5761 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5821 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
