LOCUS       V045122                 8155 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045122
VERSION     V045122
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3591
                     /label="Exosc10(NM_016699)"
                     /note="Exosc10(NM_016699)"
                     /gene="Exosc10"
     CDS             3598..3687
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    3722..3946
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3992..4420
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4434..4763
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4830..5624
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5798..5919
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5968..5984)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5992..6008
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6016..6046)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6061..6082
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6370..6955)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7126..7986)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7987..8091)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcgcctc ccagtccccg ggagcatcag
      961 tccgcgccgg cgaccagtgc gaccaagcct gacgcggaga tggtactgcc tggcttcccc
     1021 gatgcagaca gcttcgtaaa gtttgcactt gggtcggtag tggcagttac caaggcatcc
     1081 gggggcctgc cacagttcgg tgacgagtat gatttttaca gaagtttccc tgccttccag
     1141 gcattctgtg agacacaagg agacaggtta ctgcagtgca tgagtcgggt aatgcagtac
     1201 catggctgtc gcagcaacat caaagaccga agtaaagtga ctgaattgga ggacaagttt
     1261 gatttattag tcgataccaa tgacgtgata ttggagagag tgggcatgtt actggatgaa
     1321 gcctccggtg tgaacaagca tcagcagcct gtccttcctg cagggctgca ggtccccaaa
     1381 acaatagtat ccagctggaa tcggaaggca ggagagtatg gcaaaaaggc aaaatcggag
     1441 actttccgac tgctgcatgc aaaaaacatc gtgcgacctc agctcaggtt tcgagagaag
     1501 atcgacaatt ctaacacacc atttctcccg aagatctttg tcaaacccaa tgcccggaag
     1561 ccactccctc tggctctctc aaaagaaagg cgggaacgcc cgcaggaccg tccggaggac
     1621 ctggatgtcc ccccagccct ggcagatttc atccatcagc agcgaaccca gcaggtggag
     1681 caggacatgt ttgcacaccc ttaccagtat gaactggatc actttactcc gcctcagtcg
     1741 gtgctgcaga ggccgaagcc ccagttgtac cgagctgtgg gagagactcc ctgccacttg
     1801 gtgtcgtccc tggatgagct ggtggagctc aacgagaagc tcctgggctg tcaggagttt
     1861 gccgtggact tagagcatca ctcttacaga agcttcctag gactcacctg cctcatgcag
     1921 atctccaccc ggacagaaga cttcattgtc gacaccctcg agcttcgcag tgacatgtac
     1981 attctcaacg agagcctcac ggacccagcc atcgttaagg tcttccatgg tgccgactct
     2041 gacattgagt ggctgcagaa agacttcgga ctctatgtgg tgaacatgtt tgacacacac
     2101 caggcagcac ggcttctcaa cctggctcgg cactcactcg accatctgct gagactctac
     2161 tgcggtgtgg aatcaaacaa gcaatatcag ctggcagact ggaggatacg tcctctgcca
     2221 gaggaaatgc tgagctacgc ccgggatgac acccattacc tgctctacat ctatgaccga
     2281 atgaggctgg aactgtggga aagaggcaac caccagcctg tccagttgca ggtggtttgg
     2341 cagcggagca gggacatctg cctcaagaaa tttgtcaagc ctatctttac ggacgagtcc
     2401 tacctggagc tctatcgaaa gcaaaagaag cacctgaact cgcagcagct gaccgccttc
     2461 cagctgctgt ttgcttggag ggataagaca gctcgcaggg aggatgagag ctacggatat
     2521 gttctaccaa atcacatgat gctaaagata gctgaggaac tgcctaagga gcctcaaggc
     2581 atcatagctt gctgtaaccc agtaccacct cttgtccggc agcagatcaa tgagatgcat
     2641 ctcctaatcc agcaggctcg agagatgccc ctgctcaagt ctgaaaatgc agctggagtg
     2701 aggaagagcg gaccactgcc tagcgccgag agattggaga atgatctctt tggaccccat
     2761 gactgttccc acgcccctcc agataactac cagaacacct caactgatgg gaccctgcca
     2821 cttcagaagc agccaagcct attcactgag ggcaaagaag agacctctgt ggatgccgga
     2881 tgcctccttg ccacagccgt catcactctg ttcagtgaac ctaacactga ggaaggtgga
     2941 aagactccat tgacggtggc ccagaaaaaa gcccagaaca taatgcagtc ctttgaaaac
     3001 ccatttcgga tgttcctgcc ttctctggaa cacaaggccc acatctctca agcagcaaag
     3061 tttgatcctt cttcgaaaat ctatgaaatc agcaaccgct ggaagctggc cagccaggtc
     3121 caggtccaga aagaacctaa agaagcaacc aagaagaagg tagctgagca aacagctgcc
     3181 cgggaggagg cgaaggagga ggctgcagcc ggtgtgctgg aacaggctat ccctgtgcgg
     3241 cagcaggccg cgctagaaaa tgctactaag aagagagaac gagccaccag tgacctgagg
     3301 actatagagc aaaaacagga gaagaaacga ctaaaaagct ccaagaaagc caaggaccct
     3361 gatcccccag ggaaagactt cagtccctat gactacagcc agtcagactt cagggccttt
     3421 gctggggaca gtaaatccaa gccttcctcc cagttcgacc ccaataagct ggcaccttcc
     3481 ggcaagaaag gtgttggagc caaaaaatgt aaacagtcgg tgggaaacaa aagcatgtca
     3541 tttgctgttg gaaagtcgga cagaggcttc cgccacaact ggccaaagag actcgaggaa
     3601 caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga agatttagag
     3661 caaaagttaa tttccgagga ggacttatga gggcccgttt aaacccgctg atcagcctcg
     3721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3961 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4021 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4081 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4141 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4201 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4261 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4321 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4381 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4441 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4501 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4561 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4681 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4741 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4801 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4861 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4921 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4981 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5041 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5101 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5161 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5221 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5281 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5341 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5401 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5461 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5521 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5581 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5641 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5701 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5761 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5821 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5881 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5941 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6001 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6061 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6121 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6181 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6901 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7381 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7441 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7501 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7561 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7621 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7681 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7741 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7801 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7861 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7921 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7981 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8041 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8101 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggt
//
