LOCUS V044198 5887 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044198 VERSION V044198 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1326 /label="FABP4(NM_001442)" /note="FABP4(NM_001442)" /gene="FABP4" CDS 1333..1359 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1363..1389 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1393..1419 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1454..1678 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1724..2152 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2166..2495 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2562..3356 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3530..3651 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3700..3716) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3724..3740 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3748..3778) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3793..3814 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4102..4687) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4858..5718) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5719..5823) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgtgtgatg cttttgtagg tacctggaaa 961 cttgtctcca gtgaaaactt tgatgattat atgaaagaag taggagtggg ctttgccacc 1021 aggaaagtgg ctggcatggc caaacctaac atgatcatca gtgtgaatgg ggatgtgatc 1081 accattaaat ctgaaagtac ctttaaaaat actgagattt ccttcatact gggccaggaa 1141 tttgacgaag tcactgcaga tgacaggaaa gtcaagagca ccataacctt agatgggggt 1201 gtcctggtac atgtgcagaa atgggatgga aaatcaacca ccataaagag aaaacgagag 1261 gatgataaac tggtggtgga atgcgtcatg aaaggcgtca cttccacgag agtttatgag 1321 agagcactcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct 1381 gattatgcta gctatcctta tgatgtccca gactacgcat gagggcccgt ttaaacccgc 1441 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 1501 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 1561 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 1621 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 1681 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 1741 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 1801 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 1861 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 1921 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 1981 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 2041 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 2101 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 2161 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2221 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 2281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 2341 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 2401 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 2461 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 2521 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 2581 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 2641 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 2701 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 2761 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 2821 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 2881 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 2941 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 3001 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 3061 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 3121 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 3181 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 3241 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 3301 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 3361 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 3421 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 3481 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 3541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3661 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 3721 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 3781 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3841 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3901 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 3961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4081 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 4501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 4561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4621 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 4741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 4801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 4861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 5521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 5581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 5641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 5701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 5761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 5821 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 5881 ctatggt //