LOCUS V047111 8265 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047111 VERSION V047111 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 927..3071 /label="FOXP2(NM_014491)" /note="FOXP2(NM_014491)" /gene="FOXP2" CDS 3108..3827 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3865..4089 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4135..4563 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4577..4906 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4973..5767 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5941..6062 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6111..6127) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6135..6151 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6159..6189) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6204..6225 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6513..7098) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7269..8129) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8130..8234) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccatga tgcaggaatc tgcgacagag acaataagca 961 acagttcaat gaatcaaaat ggaatgagca ctctaagcag ccaattagat gctggcagca 1021 gagatggaag atcaagtggt gacaccagct ctgaagtaag cacagtagaa ctgctgcatc 1081 tgcaacaaca gcaggctctc caggcagcaa gacaacttct tttacagcag caaacaagtg 1141 gattgaaatc tcctaagagc agtgataaac agagaccact gcaggtgcct gtgtcagtgg 1201 ccatgatgac tccccaggtg atcacccctc agcaaatgca gcagatcctt cagcaacaag 1261 tcctgtctcc tcagcagcta caagcccttc tccaacaaca gcaggctgtc atgctgcagc 1321 agcaacaact acaagagttt tacaagaaac agcaagagca gttacatctt cagcttttgc 1381 agcagcagca gcaacagcag cagcagcaac aacagcagca acaacagcag cagcaacaac 1441 aacaacaaca gcagcaacaa cagcagcagc agcagcaaca gcagcagcag cagcaacagc 1501 atcctggaaa gcaagcgaaa gagcagcagc agcagcagca gcagcaacag caattggcag 1561 cccagcagct tgtcttccag cagcagcttc tccagatgca acaactccag cagcagcagc 1621 atctgctcag ccttcagcgt cagggactca tctccattcc acctggccag gcagcacttc 1681 ctgtccaatc gctgcctcaa gctggcttaa gtcctgctga gattcagcag ttatggaaag 1741 aagtgactgg agttcacagt atggaagaca atggcattaa acatggaggg ctagacctca 1801 ctactaacaa ttcctcctcg actacctcct ccaacacttc caaagcatca ccaccaataa 1861 ctcatcattc catagtgaat ggacagtctt cagttctaag tgcaagacga gacagctcgt 1921 cacatgagga gactggggcc tctcacactc tctatggcca tggagtttgc aaatggccag 1981 gctgtgaaag catttgtgaa gattttggac agtttttaaa gcaccttaac aatgaacacg 2041 cattggatga ccgaagcact gctcagtgtc gagtgcaaat gcaggtggtg caacagttag 2101 aaatacagct ttctaaagaa cgcgaacgtc ttcaagcaat gatgacccac ttgcacatgc 2161 gaccctcaga gcccaaacca tctcccaaac ctctaaatct ggtgtctagt gtcaccatgt 2221 cgaagaatat gttggagaca tccccacaga gcttacctca aacccctacc acaccaacgg 2281 ccccagtcac cccgattacc cagggaccct cagtaatcac cccagccagt gtgcccaatg 2341 tgggagccat acgaaggcga cattcagaca aatacaacat tcccatgtca tcagaaattg 2401 ccccaaacta tgaattttat aaaaatgcag atgtcagacc tccatttact tatgcaactc 2461 tcataaggca ggctatcatg gagtcatctg acaggcagtt aacacttaat gaaatttaca 2521 gctggtttac acggacattt gcttacttca ggcgtaatgc agcaacttgg aagaatgcag 2581 tacgtcataa tcttagcctg cacaagtgtt ttgttcgagt agaaaatgtt aaaggagcag 2641 tatggactgt ggatgaagta gaataccaga agcgaaggtc acaaaagata acaggaagtc 2701 caaccttagt aaaaaatata cctaccagtt taggctatgg agcagctctt aatgccagtt 2761 tgcaggctgc cttggcagag agcagtttac ctttgctaag taatcctgga ctgataaata 2821 atgcatccag tggcctactg caggccgtcc acgaagacct caatggttct ctggatcaca 2881 ttgacagcaa tggaaacagt agtccgggct gctcacctca gccgcacata cattcaatcc 2941 acgtcaagga agagccagtg attgcagagg atgaagactg cccaatgtcc ttagtgacaa 3001 cagctaatca cagtccagaa ttagaagacg acagagagat tgaagaagag cctttatctg 3061 aagatctgga actcgagggc ggtggaggaa gcggcggagg tggatctatg gtgagcaagg 3121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 3721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagggcccg 3841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 4021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 4081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4921 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 5041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 5101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 5161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 5221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 5281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 5341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 6001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6061 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 6121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 6301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 6361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 6961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7021 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 7081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 7141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 8041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 8101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 8161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc //