LOCUS       V047111                 8265 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047111
VERSION     V047111
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             927..3071
                     /label="FOXP2(NM_014491)"
                     /note="FOXP2(NM_014491)"
                     /gene="FOXP2"
     CDS             3108..3827
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3865..4089
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4135..4563
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4577..4906
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4973..5767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5941..6062
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6111..6127)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6135..6151
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6159..6189)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6204..6225
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6513..7098)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7269..8129)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8130..8234)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaagcc actgggatcc gccaccatga tgcaggaatc tgcgacagag acaataagca
      961 acagttcaat gaatcaaaat ggaatgagca ctctaagcag ccaattagat gctggcagca
     1021 gagatggaag atcaagtggt gacaccagct ctgaagtaag cacagtagaa ctgctgcatc
     1081 tgcaacaaca gcaggctctc caggcagcaa gacaacttct tttacagcag caaacaagtg
     1141 gattgaaatc tcctaagagc agtgataaac agagaccact gcaggtgcct gtgtcagtgg
     1201 ccatgatgac tccccaggtg atcacccctc agcaaatgca gcagatcctt cagcaacaag
     1261 tcctgtctcc tcagcagcta caagcccttc tccaacaaca gcaggctgtc atgctgcagc
     1321 agcaacaact acaagagttt tacaagaaac agcaagagca gttacatctt cagcttttgc
     1381 agcagcagca gcaacagcag cagcagcaac aacagcagca acaacagcag cagcaacaac
     1441 aacaacaaca gcagcaacaa cagcagcagc agcagcaaca gcagcagcag cagcaacagc
     1501 atcctggaaa gcaagcgaaa gagcagcagc agcagcagca gcagcaacag caattggcag
     1561 cccagcagct tgtcttccag cagcagcttc tccagatgca acaactccag cagcagcagc
     1621 atctgctcag ccttcagcgt cagggactca tctccattcc acctggccag gcagcacttc
     1681 ctgtccaatc gctgcctcaa gctggcttaa gtcctgctga gattcagcag ttatggaaag
     1741 aagtgactgg agttcacagt atggaagaca atggcattaa acatggaggg ctagacctca
     1801 ctactaacaa ttcctcctcg actacctcct ccaacacttc caaagcatca ccaccaataa
     1861 ctcatcattc catagtgaat ggacagtctt cagttctaag tgcaagacga gacagctcgt
     1921 cacatgagga gactggggcc tctcacactc tctatggcca tggagtttgc aaatggccag
     1981 gctgtgaaag catttgtgaa gattttggac agtttttaaa gcaccttaac aatgaacacg
     2041 cattggatga ccgaagcact gctcagtgtc gagtgcaaat gcaggtggtg caacagttag
     2101 aaatacagct ttctaaagaa cgcgaacgtc ttcaagcaat gatgacccac ttgcacatgc
     2161 gaccctcaga gcccaaacca tctcccaaac ctctaaatct ggtgtctagt gtcaccatgt
     2221 cgaagaatat gttggagaca tccccacaga gcttacctca aacccctacc acaccaacgg
     2281 ccccagtcac cccgattacc cagggaccct cagtaatcac cccagccagt gtgcccaatg
     2341 tgggagccat acgaaggcga cattcagaca aatacaacat tcccatgtca tcagaaattg
     2401 ccccaaacta tgaattttat aaaaatgcag atgtcagacc tccatttact tatgcaactc
     2461 tcataaggca ggctatcatg gagtcatctg acaggcagtt aacacttaat gaaatttaca
     2521 gctggtttac acggacattt gcttacttca ggcgtaatgc agcaacttgg aagaatgcag
     2581 tacgtcataa tcttagcctg cacaagtgtt ttgttcgagt agaaaatgtt aaaggagcag
     2641 tatggactgt ggatgaagta gaataccaga agcgaaggtc acaaaagata acaggaagtc
     2701 caaccttagt aaaaaatata cctaccagtt taggctatgg agcagctctt aatgccagtt
     2761 tgcaggctgc cttggcagag agcagtttac ctttgctaag taatcctgga ctgataaata
     2821 atgcatccag tggcctactg caggccgtcc acgaagacct caatggttct ctggatcaca
     2881 ttgacagcaa tggaaacagt agtccgggct gctcacctca gccgcacata cattcaatcc
     2941 acgtcaagga agagccagtg attgcagagg atgaagactg cccaatgtcc ttagtgacaa
     3001 cagctaatca cagtccagaa ttagaagacg acagagagat tgaagaagag cctttatctg
     3061 aagatctgga actcgagggc ggtggaggaa gcggcggagg tggatctatg gtgagcaagg
     3121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagggcccg
     3841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4921 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     4981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     5941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6061 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7021 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     7081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
