LOCUS       V020551                 7917 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020551
VERSION     V020551
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             932..3451
                     /label="GPLD1(NM_001503)"
                     /note="GPLD1(NM_001503)"
                     /gene="GPLD1"
     CDS             3452..3481
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3514..3738
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3784..4212
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4226..4555
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4622..5416
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5590..5711
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5760..5776)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5784..5800
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5808..5838)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5853..5874
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6162..6750)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6921..7781)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7782..7886)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagccac catgtctgct ttcaggttgt ggcctggcct
      961 gctgatcatg ttgggttctc tctgccatag aggttcaccg tgtggccttt caacacacgt
     1021 agaaatagga cacagagctc tggagtttct tcagcttcac aatgggcgtg ttaactacag
     1081 agagctgtta ctagaacacc aggatgcgta tcaggctgga atcgtgtttc ctgattgttt
     1141 ttaccctagc atctgcaaag gaggaaaatt ccatgatgtg tctgagagca ctcactggac
     1201 tccgtttctt aatgcaagcg ttcattatat ccgagagaac tatccccttc cctgggagaa
     1261 ggacacagag aaactggtag ctttcttgtt tggaattact tctcacatgg cggcagatgt
     1321 cagctggcat agtctgggcc ttgaacaagg attccttagg accatgggag ctattgattt
     1381 tcacggctcc tattcagagg ctcattcggc tggtgatttt ggaggagatg tgttgagcca
     1441 gtttgaattt aattttaatt accttgcacg acgctggtat gtgccagtca aagatctact
     1501 gggaatttat gagaaactgt atggtcgaaa agtcatcacc gaaaatgtaa tcgttgattg
     1561 ttcacatatc cagttcttag aaatgtatgg tgagatgcta gctgtttcca agttatatcc
     1621 cacttactct acaaagtccc cgtttttggt ggaacaattc caagagtatt ttcttggagg
     1681 actggatgat atggcatttt ggtccactaa tatttaccat ctaacaagct tcatgttgga
     1741 gaatgggacc agtgactgca acctgcctga gaaccctctg ttcattgcat gtggcggcca
     1801 gcaaaaccac acccagggct caaaaatgca gaaaaatgat tttcacagaa atttgactac
     1861 atccctaact gaaagtgttg acaggaatat aaactatact gaaagaggag tgttctttag
     1921 tgtaaattcc tggaccccgg attccatgtc ctttatctac aaggctttgg aaaggaacat
     1981 aaggacaatg ttcataggtg gctctcagtt gtcacaaaag cacgtctcca gccccttagc
     2041 atcttacttc ttgtcatttc cttatgcgag gcttggctgg gcaatgacct cagctgacct
     2101 caaccaggat gggcacggtg acctcgtggt gggcgcacca ggctacagcc gccccggcca
     2161 catccacatc gggcgcgtgt acctcatcta cggcaatgac ctgggcctgc cacctgttga
     2221 cctggacctg gacaaggagg cccacaggat ccttgaaggc ttccagccct caggtcggtt
     2281 tggctcggcc ttggctgtgt tggactttaa cgtggacggc gtgcctgacc tggccgtggg
     2341 agctccctcg gtgggctccg agcagctcac ctacaaaggt gccgtgtatg tctactttgg
     2401 ttccaaacaa ggaggaatgt cttcttcccc taacatcacc atttcttgcc aggacatcta
     2461 ctgtaacttg ggctggactc tcttggctgc agatgtgaat ggagacagtg aacccgatct
     2521 ggtcatcggc tccccttttg caccaggtgg agggaagcag aagggaattg tggctgcgtt
     2581 ttattctggc cccagcctga gcgacaaaga aaaactgaac gtggaggcag ccaactggac
     2641 ggtgagaggc gaggaagact tctcctggtt tggatattcc cttcacggtg tcactgtgga
     2701 caacagaacc ttgctgttgg ttgggagccc gacctggaag aatgccagca ggctgggcca
     2761 tttgttacac atccgagatg agaaaaagag ccttgggagg gtgtatggct acttcccacc
     2821 aaacggccaa agctggttta ccatttctgg agacaaggca atggggaaac tgggtacttc
     2881 cctttccagt ggccacgtac tgatgaatgg gactctgaaa caagtgctgc tggttggagc
     2941 ccctacgtac gatgacgtgt ctaaggtggc attcctgacc gtgaccctac accaaggcgg
     3001 agccactcgc atgtacgcac tcacatctga cgcgcagcct ctgctgctca gcaccttcag
     3061 cggagaccgc cgcttctccc gatttggtgg cgttctgcac ttgagtgacc tggatgatga
     3121 tggcttagat gaaatcatca tggcagcccc cctgaggata gcagatgtaa cctctggact
     3181 gattggggga gaagacggcc gagtatatgt atataatggc aaagagacca cccttggtga
     3241 catgactggc aaatgcaaat catggataac tccatgtcca gaagaaaagg cccaatatgt
     3301 attgatttct cctgaagcca gctcaaggtt tgggagctcc ctcatcaccg tgaggtccaa
     3361 ggcaaagaac caagtcgtca ttgctgctgg aaggagttct ttgggagccc gactctccgg
     3421 ggcacttcac gtctatagcc ttggctcaga tgaacaaaaa ctcatctcag aagaggatct
     3481 gtgacttaag tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3541 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3601 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3661 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3721 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     3781 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3841 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3961 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4021 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4081 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4141 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4201 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4561 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4621 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4681 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4741 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4801 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4861 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4921 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4981 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5041 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5101 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5161 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5221 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5281 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5341 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5401 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5461 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5521 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5581 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5761 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5821 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5881 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5941 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6001 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6061 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6601 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7021 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7201 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
