LOCUS       V036067                 9086 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036067
VERSION     V036067
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4606
                     /label="GRIN2C(NM_000835)"
                     /note="GRIN2C(NM_000835)"
                     /gene="GRIN2C"
     polyA_signal    4653..4877
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4923..5351
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5365..5694
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5761..6555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6729..6850
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6899..6915)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6923..6939
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6947..6977)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6992..7013
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7301..7886)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8057..8917)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8918..9022)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg ggtggggccc tggggccggc cctgttgctc acctcgctct tcggtgcctg
      961 ggcagggctg ggtccggggc agggcgagca gggcatgacg gtggccgtgg tgtttagcag
     1021 ctcagggccg ccccaggccc agttccgtgc ccgcctcacc ccccagagct tcctggacct
     1081 acccctggag atccagccgc tcacagttgg ggtcaacacc accaacccca gcagcctcct
     1141 cacccagatc tgcggcctcc tgggtgctgc ccacgtccac ggcattgtct ttgaggacaa
     1201 cgtggacacc gaggcggtgg cccagatcct tgacttcatc tcctcccaga cccatgtgcc
     1261 catcctcagc atcagcggag gctctgctgt ggtcctcacc cccaaggagc cgggctccgc
     1321 cttcctgcag ctgggcgtgt ccctggagca gcagctgcag gtgctgttca aggtgctgga
     1381 agagtacgac tggagcgcct tcgccgtcat caccagcctg cacccgggcc acgcgctctt
     1441 cctggagggc gtgcgcgccg tcgccgacgc cagccacgtg agttggcggc tgctggacgt
     1501 ggtcacgctg gagctgggcc cgggagggcc gcgcgcgcgc acgcagcgcc tgctgcgcca
     1561 gctcgacgcg cccgtgtttg tggcctactg ctcgcgcgag gaggccgagg tgctcttcgc
     1621 cgaggcggcg caggccggtc tggtggggcc cggccacgtg tggctggtgc ccaacctggc
     1681 gctgggcagc accgatgcgc cccccgccac cttccccgtg ggcctcatca gcgtcgtcac
     1741 cgagagctgg cgcctcagcc tgcgccagaa ggtgcgcgac ggcgtggcca ttctggccct
     1801 gggcgcccac agctactggc gccagcatgg aaccctgcca gccccggccg gggactgccg
     1861 tgttcaccct gggcccgtca gccctgcccg ggaggccttc tacaggcacc tactgaatgt
     1921 cacctgggag ggccgagact tctccttcag ccctggtggg tacctggtcc agcccaccat
     1981 ggtggtgatc gccctcaacc ggcaccgcct ctgggagatg gtggggcgct gggagcatgg
     2041 cgtcctatac atgaagtacc ccgtgtggcc tcgctacagt gcctctctgc agcctgtggt
     2101 ggacagtcgg cacctgacgg tggccacgct ggaagagcgg ccctttgtca tcgtggagag
     2161 ccctgaccct ggcacaggag gctgtgtccc caacaccgtg ccctgccgca ggcagagcaa
     2221 ccacaccttc agcagcgggg acgtggcccc ctacaccaag ctctgctgta agggattctg
     2281 catcgacatc ctcaagaagc tggccagagt ggtcaaattc tcctacgacc tgtacctggt
     2341 gaccaacggc aagcatggca agcgggtgcg cggcgtatgg aacggcatga ttggggaggt
     2401 gtactacaag cgggcagaca tggccatcgg ctccctcacc atcaatgagg aacgctccga
     2461 gatcgtagac ttctctgtac cctttgtgga gacgggcatc agtgtgatgg tggctcgcag
     2521 caatggcacc gtctccccct cggccttctt ggagccatat agccctgcag tgtgggtgat
     2581 gatgtttgtc atgtgcctca ctgtggtggc catcaccgtc ttcatgttcg agtacttcag
     2641 ccctgtcagc tacaaccaga acctcaccag aggcaagaag tccgggggcc cagctttcac
     2701 tatcggcaag tccgtgtggc tgctgtgggc gctggtcttc aacaactcag tgcccatcga
     2761 gaacccgcgg ggcaccacca gcaagatcat ggttctggtc tgggccttct ttgctgtcat
     2821 cttcctcgcc agctacacgg ccaacctggc cgccttcatg atccaagagc aatacatcga
     2881 cactgtgtcg ggcctcagtg acaagaagtt tcagcggcct caagatcagt acccaccttt
     2941 ccgcttcggc acggtgccca acggcagcac ggagcggaac atccgcagta actaccgtga
     3001 catgcacacc cacatggtca agttcaacca gcgctcggtg gaggacgcgc tcaccagcct
     3061 caagatgggg aagctggatg ccttcatcta tgatgctgct gtcctcaact acatggcagg
     3121 caaggacgag ggctgcaagc tggtcaccat tgggtctggc aaggtctttg ctaccactgg
     3181 ctacggcatc gccatgcaga aggactccca ctggaagcgg gccatagacc tggcgctctt
     3241 gcagttcctg ggggacggag agacacagaa actggagaca gtgtggctct cagggatctg
     3301 ccagaatgag aagaacgagg tgatgagcag caagctggac atcgacaaca tggcaggcgt
     3361 cttctacatg ctgctggtgg ccatggggct ggccctgctg gtcttcgcct gggagcacct
     3421 ggtctactgg aagctgcgcc actcggtgcc caactcatcc cagctggact tcctgctggc
     3481 tttcagcagg ggcatctaca gctgcttcag cggggtgcag agcctcgcca gcccaccgcg
     3541 gcaggccagc ccggacctca cggccagctc ggcccaggcc agcgtgctca agatgctgca
     3601 ggcagcccgc gacatggtga ccacggcggg cgtaagcagc tccctggacc gcgccactcg
     3661 caccatcgag aattggggtg gcggccgccg tgcgccccca ccgtccccct gcccgacccc
     3721 gcggtctggc cccagcccat gcctgcccac ccccgacccg cccccagagc cgagccccac
     3781 gggctgggga ccgccagacg ggggtcgcgc ggcgcttgtg cgcagggctc cgcagccccc
     3841 gggccgcccc ccgacgccgg ggccgcccct gtccgacgtc tcccgagtgt cgcgccgccc
     3901 agcctgggag gcgcggtggc cggtgcggac cgggcactgc gggaggcacc tctcggcctc
     3961 cgagcggccc ctgtcgcccg cgcgctgtca ctacagctcc tttcctcgag ccgaccgatc
     4021 cggccgcccc ttcctcccgc tcttcccgga gctggaggac ctgccgctgc tcggtccgga
     4081 gcagctggcc cggcgggagg ccctgctgca cgcggcctgg gcccggggct cgcgcccgcg
     4141 tcacgcttcc ctgcccagct ccgtggccga ggccttcgct cggcccagct cgctgcccgc
     4201 tgggtgcacc ggccccgcct gcgcccgccc cgacggccac tcggcctgca ggcgcttggc
     4261 gcaggcgcag tcgatgtgct tgccgatcta ccgggaggcc tgccaggagg gcgagcaggc
     4321 aggggccccc gcctggcagc acagacagca cgtctgcctg cacgcccacg cccacctgcc
     4381 attttgctgg ggggctgtct gtcctcacct tccaccctgt gccagccacg gctcctggct
     4441 ctccggggcc tgggggcctc tggggcacag gggcaggact ctggggctgg gcacaggcta
     4501 cagagacagt gggggactgg acgagatcag cagggtagcc cgtgggacgc aaggcttccc
     4561 gggaccctgc acctggagac ggatctccag tctggagtca gaagtgtgac tcgagtctag
     4621 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4681 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4741 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4801 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4861 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4921 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5161 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5281 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5341 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5701 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5941 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6541 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6601 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6661 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6721 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6781 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6841 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6961 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7801 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     7861 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7921 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7981 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8041 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8101 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8161 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8221 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8281 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8341 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8401 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8461 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8521 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8581 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8641 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8701 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8761 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8821 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8881 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8941 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9061 cgggagatct cccgatcccc tatggt
//
