LOCUS       V035790                 8761 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035790
VERSION     V035790
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4197
                     /label="HDAC4(NM_001378414)"
                     /note="HDAC4(NM_001378414)"
                     /gene="HDAC4"
     CDS             4204..4293
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    4328..4552
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4598..5026
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5040..5369
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5436..6230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6404..6525
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6574..6590)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6598..6614
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6622..6652)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6667..6688
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6976..7561)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7732..8592)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8593..8697)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgagctccc aaagccatcc agatggactt
      961 tctggccgag accagccagt ggagctgctg aatcctgccc gcgtgaacca catgcccagc
     1021 acggtggatg tggccacggc gctgcctctg caagtggccc cctcggcagt gcccatggac
     1081 ctgcgcctgg accaccagtt ctcactgcct gtggcagagc cggccctgcg ggagcagcag
     1141 ctgcagcagg agctcctggc gctcaagcag aagcagcaga tccagaggca gatcctcatc
     1201 gctgagttcc agaggcagca cgagcagctc tcccggcagc acgaggcgca gctccacgag
     1261 cacatcaagc aacaacagga gatgctggcc atgaagcacc agcaggagct gctggaacac
     1321 cagcggaagc tggagaggca ccgccaggag caggagctgg agaagcagca ccgggagcag
     1381 aagctgcagc agctcaagaa caaggagaag ggcaaagaga gtgccgtggc cagcacagaa
     1441 gtgaagatga agttacaaga atttgtcctc aataaaaaga aggcgctggc ccaccggaat
     1501 ctgaaccact gcatttccag cgaccctcgc tactggtacg ggaaaacgca gcacagttcc
     1561 cttgaccaga gttctccacc ccagagcgga gtgtcgacct cctataacca cccggtcctg
     1621 ggaatgtacg acgccaaaga tgacttccct cttaggaaaa cagcttctga accgaatctg
     1681 aaattacggt ccaggctaaa gcagaaagtg gccgaaagac ggagcagccc cctgttacgc
     1741 aggaaagacg ggccagtggt cactgctcta aaaaagcgtc cgttggatgt cacagactcc
     1801 gcgtgcagca gcgccccagg ctccggaccc agctcaccca acaacagctc cgggagcgtc
     1861 agcgcggaga acggtatcgc gcccgccgtc cccagcatcc cggcggagac gagtttggcg
     1921 cacagacttg tggcacgaga aggctcggcc gctccacttc ccctctacac atcgccatcc
     1981 ttgcccaaca tcacgctggg cctgcctgcc accggcccct ctgcgggcac ggcgggccag
     2041 caggacgccg agagactcac ccttcccgcc ctccagcaga ggctctccct tttccccggc
     2101 acccacctca ctccctacct gagcacctcg cccttggagc gggacggagg ggcagcgcac
     2161 agccctcttc tgcagcacat ggtcttactg gagcagccgc cggcacaagc acccctcgtc
     2221 acagactggt atctttcagg cctgggagca ctgcccctcc acgcacagtc cttggttggt
     2281 gcagaccggg tgtccccctc catccacaag ctgcggcagc accgcccact ggggcggacc
     2341 cagtcggccc cgctgcccca gaacgcccag gctctgcagc acctggtcat ccagcagcag
     2401 catcagcagt ttctggagaa acacaagcag cagttccagc agcagcaact gcagatgaac
     2461 aagatcatcc ccaagccaag cgagccagcc cggcagccgg agagccaccc ggaggagacg
     2521 gaggaggagc tccgtgagca ccaggctctg ctggacgagc cctacctgga ccggctgccg
     2581 gggcagaagg aggcgcacgc acaggccggc gtgcaggtga agcaggagcc cattgagagc
     2641 gatgaggaag aggcagagcc cccacgggag gtggagccgg gccagcgcca gcccagtgag
     2701 caggagctgc tcttcagaca gcaagccctc ctgctggagc agcagcggat ccaccagctg
     2761 aggaactacc aggcgtccat ggaggccgcc ggcatccccg tgtccttcgg cggccacagg
     2821 cctctgtccc gggcgcagtc ctcacccgcg tctgccacct tccccgtgtc tgtgcaggag
     2881 ccccccacca agccgaggtt cacgacaggc ctcgtgtatg acacgctgat gctgaagcac
     2941 cagtgcacct gcgggagtag cagcagccac cccgagcacg ccgggaggat ccagagcatc
     3001 tggtcccgcc tgcaggagac gggcctccgg ggcaaatgcg agtgcatccg cggacgcaag
     3061 gccaccctgg aggagctaca gacggtgcac tcggaagccc acaccctcct gtatggcacg
     3121 aaccccctca accggcagaa actggacagt aagaaacttc taggctcgct cgcctccgtg
     3181 ttcgtccggc tcccttgcgg tggtgttggg gtggacagtg acaccatatg gaacgaggtg
     3241 cactcggcgg gggcagcccg cctggctgtg ggctgcgtgg tagagctggt cttcaaggtg
     3301 gccacagggg agctgaagaa tggctttgct gtggtccgcc cccctggaca ccatgcggag
     3361 gagagcacgc ccatgggctt ttgctacttc aactccgtgg ccgtggcagc caagcttctg
     3421 cagcagaggt tgagcgtgag caagatcctc atcgtggact gggacgtgca ccatggaaac
     3481 gggacccagc aggctttcta cagcgacccc agcgtcctgt acatgtccct ccaccgctac
     3541 gacgatggga acttcttccc aggcagcggg gctcctgatg aggtgggcac agggcccggc
     3601 gtgggtttca acgtcaacat ggctttcacc ggcggcctgg acccccccat gggagacgct
     3661 gagtacttgg cggccttcag aacggtggtc atgccgatcg ccagcgagtt tgccccggat
     3721 gtggtgctgg tgtcatcagg cttcgatgcc gtggagggcc accccacccc tcttgggggc
     3781 tacaacctct ccgccagatg cttcgggtac ctgacgaagc agctgatggg cctggctggc
     3841 ggccggattg tcctggccct cgagggaggc cacgacctga ccgccatttg cgacgcctcg
     3901 gaagcatgtg tttctgcctt gctgggaaac gagcttgatc ctctcccaga aaaggtttta
     3961 cagcaaagac ccaatgcaaa cgctgtccgt tccatggaga aagtcatgga gatccacagc
     4021 aagtactggc gctgcctgca gcgcacaacc tccacagcgg ggcgttctct gatcgaggct
     4081 cagacttgcg agaacgaaga agccgagacg gtcaccgcca tggcctcgct gtccgtgggc
     4141 gtgaagcccg ccgaaaagag accagatgag gagcccatgg aagaggagcc gcccctgctc
     4201 gaggaacaaa aactaatatc agaggaagat ttggaacaga aactaatcag tgaagaagat
     4261 ttagagcaaa agttaatttc cgaggaggac ttatgagggc ccgtttaaac ccgctgatca
     4321 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4381 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4441 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4501 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4561 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4801 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4921 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5041 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5101 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5161 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5221 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5281 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5341 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5401 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5461 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5521 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5581 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5641 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5701 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5761 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5821 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5881 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5941 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6001 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6061 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6121 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6181 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6241 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6301 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6361 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6541 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6721 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7501 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7561 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7621 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7861 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8281 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8701 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     8761 t
//
