LOCUS       V033906                13244 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033906
VERSION     V033906
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..8737
                     /label="HECTD1(NM_015382)"
                     /note="HECTD1(NM_015382)"
                     /gene="HECTD1"
     CDS             8747..8764
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    8811..9035
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9081..9509
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9523..9852
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9919..10713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10887..11008
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11057..11073)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11081..11097
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11105..11135)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11150..11171
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11459..12044)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12215..13075)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13076..13180)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcagatgtgg acccagatac attgctggaa tggctacaga tgggacaggg
      961 agatgaaagg gacatgcaac taatagccct tgaacagcta tgcatgctgc ttttgatgtc
     1021 tgacaacgtg gatcgttgtt ttgaaacatg tcctcctcgc actttcttac cagccctttg
     1081 caaaattttt cttgatgaaa gtgctccaga caatgtatta gaggtgacag cccgtgccat
     1141 aacatactac ctggatgtat ctgcggaatg tacccgaagg attgttgggg tagatggagc
     1201 tataaaagca ctttgtaatc gtttggttgt agttgaactt aacaacagga ctagcagaga
     1261 cttagccgaa cagtgtgtaa aggtattaga actgatatgt actcgtgagt caggagcagt
     1321 ctttgaggct ggtggtttga attgtgtgct taccttcatt cgtgacagtg gacatctagt
     1381 tcataaagac accttgcact ctgctatggc tgtggtatca agactctgtg gcaaaatgga
     1441 gcctcaagat tcttctttag aaatttgtgt agaatctctg tctagtttat taaagcatga
     1501 agatcatcag gtttcagatg gagctctgcg atgctttgca tcactggctg accgatttac
     1561 ccgtcgtggt gttgacccag ctccattagc caagcatgga ttaactgagg agctgttatc
     1621 tcgaatggct gctgctggtg gtactgtttc aggaccatca tcagcatgca aaccaggtcg
     1681 cagcaccaca ggagctccat ccaccactgc agattccaaa ttgagtaatc aggtgtcaac
     1741 aattgtaagt ctgctctcaa cactttgcag aggctctccg gtagtaacac atgatcttct
     1801 gaggtcggag cttccagatt caattgaaag tgcattgcag ggtgatgaaa gatgtgtgct
     1861 tgatactatg cgtttggttg accttctctt ggtgctatta tttgaaggac gaaaagcttt
     1921 gccaaagtct agtgctggat ctacaggcag aatcccagga ctccggagat tagatagttc
     1981 tggggagcgc tcacatcggc agcttataga ttgtattcga agtaaagata ccgatgcact
     2041 tatagatgca attgacacag gagcctttga agtaaatttt atggatgatg taggtcagac
     2101 tctattaaac tgggcctctg cttttggaac tcaggaaatg gtagaatttc tttgtgagag
     2161 aggtgcagat gttaatagag gtcaaaggtc atcatcatta cattatgctg catgttttgg
     2221 aagacctcaa gtagcaaaga ctctgttacg gcatggtgca aatccagatc tgagagatga
     2281 agatgggaaa actccattag ataaagctcg agaaaggggc catagtgaag tggtagctat
     2341 tcttcagtct ccaggtgatt ggatgtgtcc agttaataaa ggagatgata agaaaaagaa
     2401 agatacaaac aaagatgaag aagaatgtaa tgagcccaaa ggagatccgg aaatggcacc
     2461 catatacttg aaaaggttat tgccagtgtt tgcacaaaca tttcagcaaa ctatgctgcc
     2521 ttcaataagg aaagcaagtc ttgctctaat tcgaaaaatg attcattttt gctctgaagc
     2581 actgttaaaa gaagtttgtg attctgatgt tggtcacaat ttgcctacaa tactagtgga
     2641 aatcactgca actgtcctgg atcaagagga tgatgatgat ggccacttgc tggctttgca
     2701 gatcataagg gatttagtag ataaaggtgg tgatatattt ttggatcagc tagccagact
     2761 tggtgtaatt agcaaagtgt caacgttggc aggtccttcc tctgatgatg agaatgaaga
     2821 ggaatcaaaa ccagaaaaag aagatgaacc acaggaagat gctaaagaat tgcaacaagg
     2881 taaaccatat cattggagag actggtcaat cattagggga agggactgct tatatatttg
     2941 gagtgatgca gcagccttgg aattatctaa tggcagtaat ggatggttca gatttatctt
     3001 ggatggaaaa cttgccacca tgtattcaag tggtagtccg gaaggtggat ctgacagttc
     3061 agaaagccga agtgaattct tagagaagtt acaaagagct cgaggccaag taaagccatc
     3121 tacttcaagt caacctatac tgtcagcacc aggacccact aaacttactg taggaaattg
     3181 gtcactgaca tgtttgaaag aaggagaaat tgctattcat aattcagatg gtcagcaagc
     3241 tacaatattg aaagaagatt tacctggttt tgtatttgaa tctaatagag gaaccaaaca
     3301 ttcatttact gcagaaactt ccctgggttc agaatttgtg actggctgga ctggcaaaag
     3361 aggcagaaaa ctgaaatcta agttagaaaa aacaaagcaa aaggtacgaa ctatggctcg
     3421 agatttatac gatgaccatt ttaaagctgt tgaaagcatg cctcgtggag tagtggtaac
     3481 actcagaaac atagcaactc agttagagtc atcttgggaa cttcatacaa atagacaatg
     3541 tattgagagt gagaacactt ggagagattt aatgaagaca gctttagaaa acctaattgt
     3601 acttttgaag gatgaaaaca caatttcacc atatgaaatg tgtagcagtg gcttggtaca
     3661 agcacttctt actgtgttaa acaatagcat ggatttggat atgaaacaag attgtagtca
     3721 actggtagaa agaataaatg tttttaaaac tgcctttagt gaaaatgaag atgatgaaag
     3781 tcgaccagca gttgcgttaa ttcgaaagtt aatagctgta ctagaatcta ttgaacgtct
     3841 acctctccat ttgtatgata caccaggatc cacatataac ctccagatac ttacaaggag
     3901 attacgattt cggttggaac gtgcacctgg tgaaactgca ttgattgaca ggactggcag
     3961 aatgttgaag atggaacctt tggctacagt tgaatctctg gaacagtacc ttttgaaaat
     4021 ggtagcaaaa cagtggtatg attttgaccg atcttcattt gtttttgttc gaaaattaag
     4081 agaaggacaa aattttatat ttcggcacca gcatgatttt gatgaaaatg gaatcattta
     4141 ctggattgga acaaatgcaa aaactgctta tgaatgggta aatccagctg cctatggact
     4201 tgtagtagta acgtcatcag aaggaagaaa tctaccttat ggccgcttag aagacatact
     4261 aagtcgtgat aattcagctt taaattgtca tagcaatgat gataagaatg cctggtttgc
     4321 catagatctg ggtctctggg tgataccatc agcatataca cttcgtcatg ctcgtggtta
     4381 tggaaggtct gcactgagaa attgggtttt ccaggtatcc aaagatggac agaactggac
     4441 ttctttgtat acccatgttg atgactgcag tctcaatgaa ccagggtcaa ctgcaacttg
     4501 gcctcttgat ccaccaaagg atgagaaaca agggtggaga catgtgagaa ttaaacagat
     4561 ggggaaaaat gccagtggac aaacacacta cctctcatta tctggattcg aactttatgg
     4621 cactgtaaat ggagtatgtg aagatcagct agggaaagca gctaaagaag cagaagctaa
     4681 tcttagacgg cagagacgtc tagtacgttc ccaggttctg aaatacatgg ttccaggagc
     4741 tcgtgttatc agaggcctgg attggaaatg gcgagatcag gatggcagcc cacagggaga
     4801 aggcactgtc acaggagaac tacacaatgg ctggattgat gtcacctggg atgctggtgg
     4861 ctcaaactct taccgtatgg gcgcagaagg aaaatttgac ctcaagcttg caccagggta
     4921 cgaccctgat acagtggcat cacccaaacc tgtttcatcc actgtttcag gcacaacgca
     4981 atcatggagc agcttggtga aaaacaactg tccagacaag acatctgctg ctgcaggctc
     5041 ctcaagtaga aaaggaagca gcagttctgt gtgtagcgtg gccagtagca gcgacatcag
     5101 cttgggttcg accaaaacgg aacggagatc agaaattgta atggaacaca gtatagtttc
     5161 aggagctgat gtccatgaac caattgttgt tctttcatct gctgaaaacg tccctcaaac
     5221 agaagtaggg tcatcttcca gtgcaagcac cagcacctta acagcggaaa cgggaagtga
     5281 aaatgctgaa aggaagttag gccctgatag ttctgttcgt actcctgggg agtctagtgc
     5341 aatatccatg ggaattgtca gtgttagttc tcctgatgtt agttcagtat ctgaattaac
     5401 taataaagaa gcagcttcac aacgacctct tagctcttca gcaagtaaca gactgtcagt
     5461 gagttctttg ttggctgctg gggcccctat gagctctagt gcaagtgtac ctaacctgtc
     5521 ctcaagagaa acatctagct tggagagttt tgtaaggaga gtggcaaaca tagcacggac
     5581 taatgccacg aacaacatga atctaagccg aagcagcagt gataacaaca ctaatacttt
     5641 ggggaggaat gtgatgagca cagcaacttc tcctcttatg ggtgctcaga gtttccctaa
     5701 tttgaccaca cctggtacta catcaacagt gactatgtca acatccagtg ttactagcag
     5761 cagcaatgta gctacagcaa caacagtttt atcagttggt caatctttaa gtaacacttt
     5821 aaccaccagc ctcacatcaa cttccagtga gagtgacaca ggtcaggaag cagaatattc
     5881 cttatatgat ttccttgata gctgccgtgc cagtactcta ttggctgagc tcgatgatga
     5941 tgaggactta cctgagccag atgaagaaga tgatgagaat gaagatgaca atcaggagga
     6001 ccaagaatac gaggaggtta tgattctgag acgcccatcc ctgcaacgtc gagctggctc
     6061 ccgctctgat gtaacgcatc atgctgttac ctcgcagcta ccacaggtac ctgctggagc
     6121 agggagccga cctattgggg agcaggaaga agaagagtac gaaactaaag gaggacgccg
     6181 gagaacatgg gatgatgatt atgtgctaaa gagacagttt tctgcattgg ttcctgcttt
     6241 tgatcctaga cctggtcgta ctaatgtcca gcagacaact gatctagaaa taccaccccc
     6301 agggacccct cattcagagc tcttggaaga agtcgaatgt actccgtcac ctcgattagc
     6361 tctcactttg aaagtaacag gtcttggaac gactcgtgaa gttgaattac cactcaccaa
     6421 tttcagatca accatctttt actatgtaca aaaattgctt caattgtcct gtaatggcaa
     6481 tgtgaaatca gataaactta ggcgtatttg ggagcccaca tacacaatca tgtacagaga
     6541 aatgaaggat tctgataaag aaaaggaaaa tggaaaaatg ggttgctggt ctatagagca
     6601 tgtggagcag taccttggca ctgatgaatt accaaagaat gacttgataa cctacctgca
     6661 gaagaatgca gacgctgctt tcctgcgcca ctggaaatta actggcacta ataaaagtat
     6721 taggaaaaac agaaattgtt ctcagctcat agctgcatat aaggattttt gtgagcatgg
     6781 aacaaagtct gggttaaacc agggggccat ttctactctt caaagtagtg atattcttaa
     6841 tttaacaaaa gaacaacctc aggccaaagc aggcaatgga cagaactctt gtggagtaga
     6901 agatgtcctt cagcttctgc gtattctata tatagttgca agtgaccctt attcaagaat
     6961 atcccaggaa gatggtgatg aacagcctca gtttactttt ccaccagatg aattcactag
     7021 caaaaaaatt acaacaaaaa tattacagca gattgaggaa ccattggcac tggcaagtgg
     7081 ggctctgcca gactggtgtg aacaattaac cagcaaatgt ccttttctaa taccatttga
     7141 aactagacag ctttatttca catgtacagc atttggcgcc tcaagagcaa tagtatggtt
     7201 acagaaccga cgtgaagcca ctgtggagcg aacgagaacc acaagcagtg ttaggcgaga
     7261 tgaccctgga gagtttcgag ttggtcgtct caagcatgaa agagtaaaag ttccacgtgg
     7321 cgagtcactg atggaatggg ctgagaatgt catgcaaata catgcagatc ggaaatcagt
     7381 tcttgaggtt gaatttttag gagaagaagg aactggcttg ggacccacat tagagtttta
     7441 tgctctggtg gcagcagaat tccagagaac tgacttggga gcttggcttt gtgatgataa
     7501 ttttccagat gatgaatctc gtcacgttga tcttggaggt ggattgaaac ctcctggata
     7561 ttatgtgcag aggtcatgtg gactgttcac agcaccattt ccacaggata gtgatgagct
     7621 tgaaaggatc acgaaactgt ttcatttcct tggaattttc ttggccaaat gcattcaaga
     7681 caatagactt gtggacttac ctatttctaa accttttttt aaacttatgt gtatgggtga
     7741 cattaaaagc aatatgagta aactgattta tgagtcacga ggtgatagag acttacactg
     7801 tactgaaagt cagtctgaag cttctacaga agaaggtcat gattcactct cggtaggaag
     7861 ctttgaagag gattcaaaat cagaatttat tcttgatccc cctaaaccaa aacccccagc
     7921 ttggtttaat ggaattttga cttgggaaga ctttgaatta gtaaacccac acagagccag
     7981 atttttaaaa gaaattaaag accttgctat caagaggcgc caaattttaa gcaacaaagg
     8041 tctttctgaa gatgagaaga acacaaaatt acaggaacta gtgctgaaga atccatcagg
     8101 ttctgggcct ccacttagca tagaggattt aggtttaaat ttccagtttt gcccttcctc
     8161 aagaatatat ggttttacag ctgtggatct caagccaagt ggtgaagatg agatgataac
     8221 aatggataat gcagaagaat atgtggattt gatgtttgac ttttgtatgc atacgggtat
     8281 tcagaaacaa atggaagcct ttagagatgg gtttaataaa gtttttccaa tggagaaatt
     8341 aagttccttc agccatgaag aagtccaaat gattctttgt ggaaaccagt caccatcctg
     8401 ggcagcagag gatattatca attacactga acctaagctg ggttatacac gtgacagccc
     8461 tggtttcctg aggtttgtga gggttttatg tggcatgtct tctgatgaaa ggaaagcatt
     8521 cttgcagttt accactggtt gttcaactct acccccaggt ggactggcta acctgcatcc
     8581 caggctcacg gttgtacgca aggttgatgc tactgatgca agctatccat cagtcaatac
     8641 atgtgtgcat taccttaagt tgcctgaata ttcttccgag gagatcatga gagagcgcct
     8701 gctagctgct acaatggaga aaggctttca tctcaatgcg gccgcacatc accatcatca
     8761 ccattgactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     8821 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     8881 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     8941 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     9001 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     9061 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9121 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9181 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9241 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9301 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9361 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9421 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9481 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     9541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9781 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9841 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     9901 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     9961 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    10021 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    10081 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
    10141 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    10201 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    10261 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    10321 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    10381 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    10441 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    10501 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    10561 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    10621 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    10681 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    10741 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    10801 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10861 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    10921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10981 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    11041 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    11101 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    11161 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    11221 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    11281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    11341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11881 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
    12001 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    12061 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    12121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    12181 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    12241 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    12301 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    12361 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    12421 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12481 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12541 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12601 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12661 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12721 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12781 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12841 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12901 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12961 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    13021 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    13081 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    13141 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    13201 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggt
//
