LOCUS       V039767                 8459 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039767
VERSION     V039767
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3979
                     /label="HERC5(NM_016323)"
                     /note="HERC5(NM_016323)"
                     /gene="HERC5"
     polyA_signal    4026..4250
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4296..4724
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4738..5067
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5134..5928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6102..6223
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6272..6288)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6296..6312
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6320..6350)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6365..6386
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6674..7259)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7430..8290)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8291..8395)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gagcggaggt cgcggaggaa gtcgcggcgc aacgggcgct cgaccgcggg
      961 caaggccgcc gcgacccagc ccgcgaagtc tccgggcgca cagctctggc tctttcccag
     1021 cgccgcgggc ctccaccgcg cgctgctccg gagggtggag gtgacgcgcc aactctgctg
     1081 ctcgccgggg cgcctcgcgg tcttggaacg cggcggggcg ggcgtccagg ttcaccagct
     1141 gctcgccggg agcggcggcg cccggacgcc gaaatgcatt aaattaggaa aaaacatgaa
     1201 gatacattcc gtggaccaag gagcagagca catgctgatt ctctcatcag atggaaaacc
     1261 atttgagtat gacaactata gcatgaaaca tctaaggttt gaaagcattt tacaagaaaa
     1321 aaaaataatt cagatcacat gtggagatta ccattctctt gcactctcaa aaggtggtga
     1381 gctttttgcc tggggacaga acctgcatgg gcagcttgga gttggaagga aatttccctc
     1441 aaccaccaca ccacagattg tggagcacct cgcaggagta cccttggctc agatttctgc
     1501 cggagaagcc cacagcatgg ccttatccat gtctggcaac atttattcat ggggaaaaaa
     1561 tgaatgtgga caactaggcc tgggccacac tgagagtaaa gatgatccat cccttattga
     1621 aggactagac aatcagaaag ttgaatttgt cgcttgtggt ggctctcaca gtgccctact
     1681 cacacaggat gggctgctgt ttactttcgg tgctggaaaa catgggcaac ttggtcataa
     1741 ttcaacacag aatgagctaa gaccctgttt ggtggctgag cttgttgggt atagagtgac
     1801 tcagatagca tgtggaaggt ggcacacact tgcctatgtt tctgatttgg gaaaggtctt
     1861 ttcctttggt tctggaaaag atggacaact gggaaatggt ggaacacgtg accagctgat
     1921 gccgcttcca gtgaaagtat catcaagtga agaactcaaa cttgaaagcc atacctcaga
     1981 aaaggagtta ataatgattg ctggagggaa tcaaagcatt ttgctctgga taaagaaaga
     2041 gaattcatat gttaatctga agaggacaat tcctactctg aatgaaggga ctgtaaagag
     2101 atggattgct gatgtggaga ctaaacggtg gcagagcaca aaaagggaaa tccaagagat
     2161 attttcatct cctgcttgtc taactggaag ttttttaagg aaaagaagaa ctacagaaat
     2221 gatgcctgtt tatttggact taaataaagc aagaaacatc ttcaaggagt taacccaaaa
     2281 ggactggatt actaacatga taaccacctg cctcaaagat aatctgctca aaagacttcc
     2341 atttcattct ccaccccaag aagctttaga aattttcttc cttctcccag aatgtcctat
     2401 gatgcatatt tccaacaact gggagagcct tgtggttcca tttgcaaagg ttgtttgtaa
     2461 aatgagtgac cagtcttcac tggttctgga agagtattgg gcaactctgc aagaatccac
     2521 tttcagcaaa ctggtccaga tgtttaaaac agccgtcata tgccagttgg attactggga
     2581 tgaaagtgct gaggagaatg gtaatgttca agctctccta gaaatgttga agaagctgca
     2641 cagggtaaac caggtgaaat gtcaactacc tgaaagtatt ttccaagtag acgaactctt
     2701 gcaccgtctc aatttttttg tagaagtatg cagaaggtac ttgtggaaaa tgactgtgga
     2761 cgcttcagaa aatgtacaat gctgcgtcat attcagtcac tttccattta tctttaataa
     2821 tctgtcgaaa attaaactac tacatacaga cacactttta aaaatagaga gtaaaaaaca
     2881 taaagcttat cttaggtcgg cagcaattga ggaagaaaga gagtctgaat tcgctttgag
     2941 gcccacgttt gatctaacag tcagaaggaa tcacttgatt gaggatgttt tgaatcagct
     3001 aagtcaattt gagaatgaag acctgaggaa agagttatgg gtttcattta gtggagaaat
     3061 tgggtatgac ctcggaggag tcaagaaaga gttcttctac tgtctgtttg cagagatgat
     3121 ccagccggaa tatgggatgt tcatgtatcc tgaaggggct tcctgcatgt ggtttcctgt
     3181 caagcctaaa tttgagaaga aaagatactt cttttttggg gttctatgtg gactttccct
     3241 gttcaattgc aatgttgcca accttccttt cccactggca ctgtttaaga aacttttgga
     3301 ccaaatgcca tcattggaag acttgaaaga actcagtcct gatttgggaa agaatttgca
     3361 aacacttctg gatgatgaag gtgataactt tgaggaagta ttttacatcc attttaatgt
     3421 gcactgggac agaaacgaca caaacttaat tcctaatgga agtagcataa ctgtcaacca
     3481 gactaacaag agagactatg tttctaagta tatcaattac attttcaacg actctgtaaa
     3541 ggcggtttat gaagaatttc ggagaggatt ttataaaatg tgcgacgaag acattatcaa
     3601 attattccac cccgaagaac tgaaggatgt gattgttgga aatacagatt atgattggaa
     3661 aacatttgaa aagaatgcac gttatgaacc aggatataac agttcacatc ccaccatagt
     3721 gatgttttgg aaggctttcc acaaattgac tctggaagaa aagaaaaaat tccttgtatt
     3781 tcttacagga actgacagac tacaaatgaa agatttaaat aatatgaaaa taacattttg
     3841 ctgtcctgaa agttggaatg aaagagaccc tataagagca ctgacatgtt tcagtgtcct
     3901 cttcctccct aaatattcta caatggaaac agttgaagaa gcgcttcaag aagccatcaa
     3961 caacaacaga ggatttggct gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     4021 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4081 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4141 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4201 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4261 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4981 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5041 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5101 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5161 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5221 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5281 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5341 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5401 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5461 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5521 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5581 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5641 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5701 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5761 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5821 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5881 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5941 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6001 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6061 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6241 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6301 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6361 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6421 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6481 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7201 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8401 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
