LOCUS V039767 8459 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039767 VERSION V039767 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..3979 /label="HERC5(NM_016323)" /note="HERC5(NM_016323)" /gene="HERC5" polyA_signal 4026..4250 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4296..4724 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4738..5067 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5134..5928 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6102..6223 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6272..6288) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6296..6312 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6320..6350) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6365..6386 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6674..7259) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7430..8290) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8291..8395) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gagcggaggt cgcggaggaa gtcgcggcgc aacgggcgct cgaccgcggg 961 caaggccgcc gcgacccagc ccgcgaagtc tccgggcgca cagctctggc tctttcccag 1021 cgccgcgggc ctccaccgcg cgctgctccg gagggtggag gtgacgcgcc aactctgctg 1081 ctcgccgggg cgcctcgcgg tcttggaacg cggcggggcg ggcgtccagg ttcaccagct 1141 gctcgccggg agcggcggcg cccggacgcc gaaatgcatt aaattaggaa aaaacatgaa 1201 gatacattcc gtggaccaag gagcagagca catgctgatt ctctcatcag atggaaaacc 1261 atttgagtat gacaactata gcatgaaaca tctaaggttt gaaagcattt tacaagaaaa 1321 aaaaataatt cagatcacat gtggagatta ccattctctt gcactctcaa aaggtggtga 1381 gctttttgcc tggggacaga acctgcatgg gcagcttgga gttggaagga aatttccctc 1441 aaccaccaca ccacagattg tggagcacct cgcaggagta cccttggctc agatttctgc 1501 cggagaagcc cacagcatgg ccttatccat gtctggcaac atttattcat ggggaaaaaa 1561 tgaatgtgga caactaggcc tgggccacac tgagagtaaa gatgatccat cccttattga 1621 aggactagac aatcagaaag ttgaatttgt cgcttgtggt ggctctcaca gtgccctact 1681 cacacaggat gggctgctgt ttactttcgg tgctggaaaa catgggcaac ttggtcataa 1741 ttcaacacag aatgagctaa gaccctgttt ggtggctgag cttgttgggt atagagtgac 1801 tcagatagca tgtggaaggt ggcacacact tgcctatgtt tctgatttgg gaaaggtctt 1861 ttcctttggt tctggaaaag atggacaact gggaaatggt ggaacacgtg accagctgat 1921 gccgcttcca gtgaaagtat catcaagtga agaactcaaa cttgaaagcc atacctcaga 1981 aaaggagtta ataatgattg ctggagggaa tcaaagcatt ttgctctgga taaagaaaga 2041 gaattcatat gttaatctga agaggacaat tcctactctg aatgaaggga ctgtaaagag 2101 atggattgct gatgtggaga ctaaacggtg gcagagcaca aaaagggaaa tccaagagat 2161 attttcatct cctgcttgtc taactggaag ttttttaagg aaaagaagaa ctacagaaat 2221 gatgcctgtt tatttggact taaataaagc aagaaacatc ttcaaggagt taacccaaaa 2281 ggactggatt actaacatga taaccacctg cctcaaagat aatctgctca aaagacttcc 2341 atttcattct ccaccccaag aagctttaga aattttcttc cttctcccag aatgtcctat 2401 gatgcatatt tccaacaact gggagagcct tgtggttcca tttgcaaagg ttgtttgtaa 2461 aatgagtgac cagtcttcac tggttctgga agagtattgg gcaactctgc aagaatccac 2521 tttcagcaaa ctggtccaga tgtttaaaac agccgtcata tgccagttgg attactggga 2581 tgaaagtgct gaggagaatg gtaatgttca agctctccta gaaatgttga agaagctgca 2641 cagggtaaac caggtgaaat gtcaactacc tgaaagtatt ttccaagtag acgaactctt 2701 gcaccgtctc aatttttttg tagaagtatg cagaaggtac ttgtggaaaa tgactgtgga 2761 cgcttcagaa aatgtacaat gctgcgtcat attcagtcac tttccattta tctttaataa 2821 tctgtcgaaa attaaactac tacatacaga cacactttta aaaatagaga gtaaaaaaca 2881 taaagcttat cttaggtcgg cagcaattga ggaagaaaga gagtctgaat tcgctttgag 2941 gcccacgttt gatctaacag tcagaaggaa tcacttgatt gaggatgttt tgaatcagct 3001 aagtcaattt gagaatgaag acctgaggaa agagttatgg gtttcattta gtggagaaat 3061 tgggtatgac ctcggaggag tcaagaaaga gttcttctac tgtctgtttg cagagatgat 3121 ccagccggaa tatgggatgt tcatgtatcc tgaaggggct tcctgcatgt ggtttcctgt 3181 caagcctaaa tttgagaaga aaagatactt cttttttggg gttctatgtg gactttccct 3241 gttcaattgc aatgttgcca accttccttt cccactggca ctgtttaaga aacttttgga 3301 ccaaatgcca tcattggaag acttgaaaga actcagtcct gatttgggaa agaatttgca 3361 aacacttctg gatgatgaag gtgataactt tgaggaagta ttttacatcc attttaatgt 3421 gcactgggac agaaacgaca caaacttaat tcctaatgga agtagcataa ctgtcaacca 3481 gactaacaag agagactatg tttctaagta tatcaattac attttcaacg actctgtaaa 3541 ggcggtttat gaagaatttc ggagaggatt ttataaaatg tgcgacgaag acattatcaa 3601 attattccac cccgaagaac tgaaggatgt gattgttgga aatacagatt atgattggaa 3661 aacatttgaa aagaatgcac gttatgaacc aggatataac agttcacatc ccaccatagt 3721 gatgttttgg aaggctttcc acaaattgac tctggaagaa aagaaaaaat tccttgtatt 3781 tcttacagga actgacagac tacaaatgaa agatttaaat aatatgaaaa taacattttg 3841 ctgtcctgaa agttggaatg aaagagaccc tataagagca ctgacatgtt tcagtgtcct 3901 cttcctccct aaatattcta caatggaaac agttgaagaa gcgcttcaag aagccatcaa 3961 caacaacaga ggatttggct gactcgagtc tagagggccc gtttaaaccc gctgatcagc 4021 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 4081 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 4141 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 4201 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 4261 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 4741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4981 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 5041 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 5101 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 5161 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 5221 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 5281 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 5341 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 5401 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 5461 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 5521 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 5581 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 5641 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc 5701 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 5761 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 5821 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 5881 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 5941 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 6001 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 6061 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 6241 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6301 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6361 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6421 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6481 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7201 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8401 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt //