LOCUS       V025769                13351 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025769
VERSION     V025769
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      901..910
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             940..8157
                     /label="HIVEP3(NM_024503)"
                     /note="HIVEP3(NM_024503)"
                     /gene="HIVEP3"
     CDS             8194..8913
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    8951..9175
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9221..9649
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9663..9992
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10059..10853
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11027..11148
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11197..11213)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11221..11237
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11245..11275)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11290..11311
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11599..12184)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12355..13215)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13216..13320)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg aacaaaaact catctcagaa gaggatctga tggatcctga acaaagtgtc
      961 aagggcacca agaaggctga gggaagtccc cggaagcggc tgaccaaagg agaggccatt
     1021 cagaccagtg tttcttccag cgtcccatac ccaggcagcg gcacagctgc cacccaagag
     1081 agccccgccc aagagctctt agccccgcag cccttcccgg gcccctcatc agttcttagg
     1141 gaaggctctc aggagaaaac gggccagcag cagaagcccc ccaaaaggcc ccccatcgaa
     1201 gcatccgtcc acatctcaca gcttccgcag caccctctga caccagcatt catgtcgcct
     1261 ggcaaacctg agcatctcct ggaggggtcc acatggcaac tggttgaccc catgagacct
     1321 ggaccctctg gctccttcgt ggcccctggg ctccatcctc agagccagct ccttccttcc
     1381 cacgcttcca tcattccccc cgaggacctt cctggagtcc ccaaagtctt cgtgcctcgt
     1441 ccttcccagg tctccttgaa gcccacagaa gaggcacaca agaaggagag gaagccccag
     1501 aagccaggca agtacatctg ccagtactgc agccggccct gtgccaagcc cagcgtgctc
     1561 cagaagcaca ttcgctcaca cacaggtgag aggccctacc cctgcggccc ctgtggcttc
     1621 tccttcaaga ccaagagtaa tctctacaag cacaggaagt cccatgccca ccgcatcaaa
     1681 gcaggcctgg cctcaggcat gggtggcgag atgtacccac atgggctgga gatggagcgg
     1741 atccctgggg aagagtttga ggagcccact gagggagaaa gcacagattc tgaagaggag
     1801 actagtgcca cctctggtca ccctgcagag ctctccccaa gacccaagca gccccttctc
     1861 tccagcgggc tatacagctc tgggagccac agttccagcc acgaacgctg ttccctgtcc
     1921 cagtccagca cagcccagtc actcgaagac ccccctccat ttgtggaacc ctcatctgag
     1981 caccccctga gccataaacc tgaagacacc cacacgatta agcagaagct ggccctccgc
     2041 ttaagcgaga ggaagaaggt gatcgatgag caggcgtttc tgagcccagg cagcaaaggg
     2101 agtactgagt ctgggtattt ctctcgctcc gagagtgcag agcagcaggt cagcccccca
     2161 aacaccaacg ccaagtccta cgctgagatc atctttggca agtgtgggcg aataggacag
     2221 cggaccgcca tgctgacagc cacctccacc cagcccctcc tgcccctgtc caccgaagac
     2281 aagcccagcc tggtgccttt gtctgtaccc cggacgcagg tgatcgagca catcacgaag
     2341 ctcatcacca tcaacgaggc cgtggtggac accagcgaga tcgacagcgt gaagccaagg
     2401 cggagctcac tgtccaggcg cagcagcatg gagtccccaa aatccagcct ctaccgggag
     2461 cccctgtcat cccacagtga gaaaaccaag cctgaacaat cactgctgag cctccagcac
     2521 ccgcccagta ccgccccccc tgtgcctctc ctgagaagcc actcaatgcc ttctgccgcc
     2581 tgcactatca gcacccccca ccaccccttc cgaggtagct actccttcga tgaccatatc
     2641 accgactccg aagccctgag ccacagcagt cacgtgttta cctcccaccc ccggatgctg
     2701 aagcgccagc cggcaatcga attacctttg ggaggggaat acagttctga ggagcctggc
     2761 ccaagcagca aagacacagc ctccaagccc tcggacgaag tggaacccaa ggaaagcgag
     2821 cttaccaaaa agaccaagaa gggtttgaaa acaaaagggg tgatctacga atgtaacata
     2881 tgtggtgctc ggtacaagaa aagggataac tacgaagccc acaaaaaata ctactgctca
     2941 gagcttcaga tcgcaaagcc catctctgca ggcacccaca catctccaga agctgaaaag
     3001 agtcagattg agcatgagcc gtggtcccaa atgatgcatt acaaactggg aaccaccctg
     3061 gaactcactc cactgaggaa gaggaggaaa gagaagagcc ttggggacga ggaagagcca
     3121 cctgcctttg agtccacaaa aagtcagttt ggcagccccg ggccatctga tgctgctcgg
     3181 aaccttcccc tggagtccac caagtcacca gcagaaccaa gtaaatcagt gccctccttg
     3241 gagggaccca cgggcttcca gccaaggact cccaagccag ggtccggttc agaatcaggg
     3301 aaggagagga gaacaacgtc caaagaaatt tctgtcatcc agcacaccag ctcctttgag
     3361 aaatctgatt ctctcgagca gccgagtggc ttggaagggg aagacaaacc tctggcccag
     3421 ttcccatcac ccccacctgc cccacacgga cgctctgctc actccctgca gcctaagttg
     3481 gtccgccagc ccaacattca ggttcctgag atcctagtaa ctgaggagcc tgaccggccg
     3541 gacacagagc cagagccgcc ccctaaggaa cctgagaaga ctgaggagtt ccaatggccc
     3601 cagcgcagcc agacacttgc ccagctccca gctgagaagc tgccacccaa aaagaagagg
     3661 ttgcgcctgg cagagatggc ccaatcatca ggggagtcca gcttcgagtc ctctgtgcct
     3721 ctgtctcgca gcccgagcca ggaaagcaat gtctctttga gtgggtccag ccgctcagcc
     3781 tcgtttgaga gggatgacca tgggaaagcc gaggccccca gtccctcatc tgacatgcgc
     3841 cccaaacccc tgggcaccca catgttgact gtccccagcc accacccaca tgcccgagag
     3901 atgcggaggt cagcctcaga gcagagcccc aacgtttccc attctgccca catgaccgag
     3961 acacgcagca aatcctttga ctatggcagc ttgtccttga caggcccttc tgctccagcc
     4021 ccagtggctc caccagcgcg ggtggccccg ccagagagaa gaaaatgctt cttggtgaga
     4081 caggcctctc tgagcaggcc tccagaatct gagttggagg ttgcccccaa gggaagacag
     4141 gagagcgaag aaccacagcc ctcatccagt aaaccctctg ccaaaagctc attgtcccag
     4201 atttcctctg cggccacctc acatggtgga cccccgggag gcaagggccc agggcaggac
     4261 aggcccccat tggggcccac tgtgccctac acagaagcac tgcaagtgtt ccaccacccc
     4321 gttgcccaga cacccctgca tgagaagcca tacctgcccc caccagtctc ccttttctcc
     4381 ttccagcatc tcgtgcagca tgagccagga cagtctccag aattcttctc cacccaggcc
     4441 atgtccagcc tcctgtcctc accatactcc atgcccccac ttcctccctc cttatttcaa
     4501 gccccaccgc ttcctctcca gcctactgtt ctgcacccag gccaactcca tctcccccag
     4561 ctcatgcctc acccagccaa catccccttc aggcagcccc cttccttcct ccccatgcca
     4621 tacccgacct cctcagcact gtcttctggg tttttcctgc ctctgcaatc ccagtttgca
     4681 cttcagctcc ctggtgatgt ggaaagccat ctgccccaga tcaaaaccag cctggcccca
     4741 ctggcaacag gaagtgctgg cctctccccc agcacagagt acagcagtga catccggcta
     4801 ccccctgtgg ctcccccagc cagctcctca gcacctacat cagctcctcc actggccctg
     4861 cctgcctgtc cagacaccat ggtgtccctg gttgtgcctg tccgtgttca gaccaatatg
     4921 ccgtcctatg ggagcgcaat gtacaccacc ctttcccaga tcttggtcac ccagtcccaa
     4981 ggcagctcag caactgtggc acttcccaag tttgaggaac ccccatcaaa ggggacgact
     5041 gtatgtggtg cagatgtgca tgaggttggg cccggccctt ctgggttaag tgaagagcaa
     5101 agcagagctt tcccaactcc atacctgaga gtgcctgtga cattacctga aagaaaaggc
     5161 acttccctgt catcagagag tatcttgagc ctggagggga gttcatcaac agcaggggga
     5221 agcaaacgtg tcctttcacc agctggcagc cttgaactta ccatggaaac ccagcagcaa
     5281 aaaagagtga aggaggagga ggcttccaag gcagatgaaa aacttgagct ggtaaaacca
     5341 tgcagtgtgg tccttaccag caccgaggat gggaagaggc cagagaaatc ccacttaggc
     5401 aaccagggcc aaggcaggag ggagctagaa atgctgtcca gcctgtcctc agatccatct
     5461 gacacaaagg aaattcctcc cctccctcac cctgcattgt cccatgggac agccccaggc
     5521 tcagaagctt tgaaggaata tccccagcca tctggcaaac ctcaccgaag agggttgacc
     5581 ccactgagcg tgaagaaaga agattccaag gaacaacctg atctcccctc cttggcacct
     5641 ccgagctctc tgcctctgtc agaaacgtcc tccagaccag ccaagtcaca agaaggtacg
     5701 gactcaaaga aggtactgca gttccccagc ctccacacaa ccactaatgt cagttggtgc
     5761 tatttaaact acattaagcc aaatcacatc cagcatgcag ataggaggtc ctctgtttac
     5821 gctggttggt gcataagttt gtacaacccc aaccttccgg gggtttccac taaagctgct
     5881 ttgtccctcc tgaggtctaa gcagaaagtg agcaaagaga catacaccat ggccacagct
     5941 ccgcatcctg aggcaggaag gcttgtgcca tccagctccc gcaagccccg catgacagag
     6001 gttcacctcc cttcactggt ttccccggaa ggccagaaag atctagctag agtggagaag
     6061 gaagaagaga ggagagggga gccggaggag gatgctcctg cctcccagag aggggagccg
     6121 gcgaggatca aaatcttcga aggagggtac aaatcaaacg aagagtatgt atatgtgcga
     6181 ggccgcggcc gagggaaata tgtttgtgag gagtgtggaa ttcgctgcaa gaagcccagc
     6241 atgctgaaga aacacatccg cacccacact gacgtccggc cctatgtgtg caagcactgt
     6301 cactttgctt ttaaaaccaa agggaatctg actaagcaca tgaagtcgaa ggcccacagc
     6361 aaaaagtgcc aagagacagg ggtgctggag gagctggaag ccgaagaagg aaccagtgac
     6421 gacctgttcc aggactcgga aggacgagag ggttcagagg ctgtggagga gcaccagttt
     6481 tcggacctgg aggactcgga ctcagactca gacctggacg aagacgagga tgaggatgag
     6541 gaggagagcc aggatgagct gtccagacca tcctcagagg cgcccccgcc tggcccacca
     6601 catgcactgc gggcagactc ctcacccatc ctgggccctc agcccccaga tgcccccgcc
     6661 tctggcacgg aggctacacg aggcagctcg gtctcggaag ctgagcgcct gacagccagc
     6721 agctgctcca tgtccagcca gagcatgccg ggcctcccct ggctgggacc ggcccctctg
     6781 ggctctgtgg agaaagacac aggctcagcc ttgagctaca agcctgtgtc cccaagaaga
     6841 ccgtggtccc caagcaaaga agcaggcagc cgtccaccac tagcccgcaa acactcgcta
     6901 accaaaaacg actcatctcc ccagcgatgc tccccggccc gagaaccaca ggcctcagcc
     6961 ccaagcccac ctggcctgca cgtggaccca ggaaggggca tgggcgctct cccttgtggg
     7021 tctccaagac ttcagctgtc tcctctcacc ctctgccccc tgggaagaga actggcccct
     7081 cgagcacatg tgctctccaa actcgagggt accaccgacc caggcctccc cagatactcg
     7141 cccaccagga gatggtctcc aggtcaggcc gagtcaccac cacggtcagc gccgccaggg
     7201 aagtgggcct tggctgggcc gggcagcccc tcagcggggg agcatggccc aggcttgggg
     7261 ctggacccac gggttctctt cccgcccgcg cctctacctc acaagctcct cagcagaagc
     7321 ccagagacct gcgcctcccc gtggcagaag gccgagtccc gaagtccctc ctgctcaccc
     7381 ggccctgctc atcctctctc ctcccgaccc ttctccgccc tccatgactt ccacggccac
     7441 atcctggccc ggacagagga gaacatcttc agccacctgc ctctgcactc ccagcacttg
     7501 acccgtgccc catgtccctt gattcccatc ggtgggatcc agatggtgca ggcccggcca
     7561 ggagcccacc ccaccctgct gccagggccc accgcagcct gggtcagtgg cttctccggg
     7621 ggtggcagcg acctgacagg ggcccgggag gcccaggagc gaggccgctg gagtcccact
     7681 gagagctcgt cagcctccgt gtcgcctgtg gctaaggtct ccaaattcac actctcctca
     7741 gagctggagg gcggggacta ccccaaggag agggagagga ccggcggagg cccgggcagg
     7801 cctcctgact ggacacccca tgggaccggg gcacctgcag agcccacacc cacgcacagc
     7861 ccctgcaccc cacccgacac cttgccccgg ccgccccagg gacgccgggc agcgcagtcc
     7921 tggagccccc gcttggagtc cccgcgtgca ccgaccaacc ccgagccttc tgccaccccg
     7981 ccgctggacc gcagcagctc tgtgggctgc ctggcagagg cctctgcccg cttcccagcc
     8041 cggacgagga acctctccgg ggaacccagg accaggcagg actcccccaa gccctcagga
     8101 agtggggagc ccagggcaca tccacatcag cctgaggaca gggttccccc caacgctgga
     8161 tccggcggtg gaggaagcgg cggaggtgga tctatggtga gcaagggcga ggagctgttc
     8221 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     8281 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     8341 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     8401 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     8461 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     8521 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     8581 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     8641 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     8701 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     8761 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     8821 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     8881 atcactctcg gcatggacga gctgtacaag tagtctagag ggcccgttta aacccgctga
     8941 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     9001 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9061 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9121 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9181 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     9241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9421 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9481 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9541 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9601 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     9661 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9721 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     9781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     9961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    10021 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
    10081 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
    10141 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
    10201 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
    10261 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
    10321 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
    10381 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
    10441 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
    10501 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
    10561 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
    10621 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
    10681 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
    10741 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
    10801 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
    10861 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
    10921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    11041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    11161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    11221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    12001 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    12061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    12121 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12181 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12241 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12301 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12361 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12421 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12481 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12541 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12601 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12661 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12721 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12781 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12841 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12901 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12961 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    13021 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    13081 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13141 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13201 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13261 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13321 cacatttccc cgaaaagtgc cacctgacgt c
//
