LOCUS       V039643                 8279 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039643
VERSION     V039643
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3102
                     /label="HSP90AB1(NM_007355)"
                     /note="HSP90AB1(NM_007355)"
                     /gene="HSP90AB1"
     CDS             3139..3795
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*"
                     /label="GST"
     polyA_signal    3846..4070
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4116..4544
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4558..4887
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4954..5748
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5922..6043
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6092..6108)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6116..6132
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6140..6170)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6185..6206
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6494..7079)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7250..8110)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8111..8215)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgcctgagg aagtgcacca tggagaggag
      961 gaggtggaga cttttgcctt tcaggcagaa attgcccaac tcatgtccct catcatcaat
     1021 accttctatt ccaacaagga gattttcctt cgggagttga tctctaatgc ttctgatgcc
     1081 ttggacaaga ttcgctatga gagcctgaca gacccttcga agttggacag tggtaaagag
     1141 ctgaaaattg acatcatccc caaccctcag gaacgtaccc tgactttggt agacacaggc
     1201 attggcatga ccaaagctga tctcataaat aatttgggaa ccattgccaa gtctggtact
     1261 aaagcattca tggaggctct tcaggctggt gcagacatct ccatgattgg gcagtttggt
     1321 gttggctttt attctgccta cttggtggca gagaaagtgg ttgtgatcac aaagcacaac
     1381 gatgatgaac agtatgcttg ggagtcttct gctggaggtt ccttcactgt gcgtgctgac
     1441 catggtgagc ccattggcag gggtaccaaa gtgatcctcc atcttaaaga agatcagaca
     1501 gagtacctag aagagaggcg ggtcaaagaa gtagtgaaga agcattctca gttcataggc
     1561 tatcccatca ccctttattt ggagaaggaa cgagagaagg aaattagtga tgatgaggca
     1621 gaggaagaga aaggtgagaa agaagaggaa gataaagatg atgaagaaaa acccaagatc
     1681 gaagatgtgg gttcagatga ggaggatgac agcggtaagg ataagaagaa gaaaactaag
     1741 aagatcaaag agaaatacat tgatcaggaa gaactaaaca agaccaagcc tatttggacc
     1801 agaaaccctg atgacatcac ccaagaggag tatggagaat tctacaagag cctcactaat
     1861 gactgggaag accacttggc agtcaagcac ttttctgtag aaggtcagtt ggaattcagg
     1921 gcattgctat ttattcctcg tcgggctccc tttgaccttt ttgagaacaa gaagaaaaag
     1981 aacaacatca aactctatgt ccgccgtgtg ttcatcatgg acagctgtga tgagttgata
     2041 ccagagtatc tcaattttat ccgtggtgtg gttgactctg aggatctgcc cctgaacatc
     2101 tcccgagaaa tgctccagca gagcaaaatc ttgaaagtca ttcgcaaaaa cattgttaag
     2161 aagtgccttg agctcttctc tgagctggca gaagacaagg agaattacaa gaaattctat
     2221 gaggcattct ctaaaaatct caagcttgga atccacgaag actccactaa ccgccgccgc
     2281 ctgtctgagc tgctgcgcta tcatacctcc cagtctggag atgagatgac atctctgtca
     2341 gagtatgttt ctcgcatgaa ggagacacag aagtccatct attacatcac tggtgagagc
     2401 aaagagcagg tggccaactc agcttttgtg gagcgagtgc ggaaacgggg cttcgaggtg
     2461 gtatatatga ccgagcccat tgacgagtac tgtgtgcagc agctcaagga atttgatggg
     2521 aagagcctgg tctcagttac caaggagggt ctggagctgc ctgaggatga ggaggagaag
     2581 aagaagatgg aagagagcaa ggcaaagttt gagaacctct gcaagctcat gaaagaaatc
     2641 ttagataaga aggttgagaa ggtgacaatc tccaatagac ttgtgtcttc accttgctgc
     2701 attgtgacca gcacctacgg ctggacagcc aatatggagc ggatcatgaa agcccaggca
     2761 cttcgggaca actccaccat gggctatatg atggccaaaa agcacctgga gatcaaccct
     2821 gaccacccca ttgtggagac gctgcggcag aaggctgagg ccgacaagaa tgataaggca
     2881 gttaaggacc tggtggtgct gctgtttgaa accgccctgc tatcttctgg cttttccctt
     2941 gaggatcccc agacccactc caaccgcatc tatcgcatga tcaagctagg tctaggtatt
     3001 gatgaagatg aagtggcagc agaggaaccc aatgctgcag ttcctgatga gatcccccct
     3061 ctcgagggcg atgaggatgc gtctcgcatg gaagaagtcg atggtggagg cggatcagga
     3121 ggcggtggat ctgatatcat gtcccctata ctaggttatt ggaaaattaa gggccttgtg
     3181 caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag
     3241 cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat
     3301 cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat
     3361 atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg
     3421 cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac
     3481 tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa
     3541 gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg
     3601 ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca
     3661 aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa
     3721 tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac
     3781 catcctccaa aatgagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     3841 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3901 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3961 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4021 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4081 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4141 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4201 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4261 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4321 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4381 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4441 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4501 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4561 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4801 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4861 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4921 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4981 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5041 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5101 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5161 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5221 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5281 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5341 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5401 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5461 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5521 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5581 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5641 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5701 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5761 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5821 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5881 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6061 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7021 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8221 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
