LOCUS       V038934                 7487 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038934
VERSION     V038934
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..2281
                     /label="HTR2C(NM_001256760)"
                     /note="HTR2C(NM_001256760)"
                     /gene="HTR2C"
     polyA_signal    3054..3278
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3324..3752
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3766..4095
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4162..4956
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5130..5251
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5300..5316)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5324..5340
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5348..5378)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5393..5414
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5702..6287)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6458..7318)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7319..7423)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gtgaacctga ggaatgcggt gcattcattc cttgtgcacc taattggcct
      961 attggtttgg caatctgata tttctgtgag cccagtagca gctatagtaa ctgacatttt
     1021 caatacctcc gatggtggac gcttcaaatt cccagacggg gtacaaaact ggccagcact
     1081 ttcaatcgtc atcataataa tcatgacaat aggtggcaac atccttgtga tcatggcagt
     1141 aagcatggaa aagaaactgc acaatgccac caattacttc ttaatgtccc tagccattgc
     1201 tgatatgcta gtgggactac ttgtcatgcc cctgtctctc ctggcaatcc tttatgatta
     1261 tgtctggcca ctacctagat atttgtgccc cgtctggatt tctttagatg ttttattttc
     1321 aacagcgtcc atcatgcacc tctgcgctat atcgctggat cggtatgtag caatacgtaa
     1381 tcctattgag catagccgtt tcaattcgcg gactaaggcc atcatgaaga ttgctattgt
     1441 ttgggcaatt tctataggtg tatcagttcc tatccctgtg attggactga gggacgaaga
     1501 aaaggtgttc gtgaacaaca cgacgtgcgt gctcaacgac ccaaatttcg ttcttattgg
     1561 gtccttcgta gctttcttca taccgctgac gattatggtg attacgtatt gcctgaccat
     1621 ctacgttctg cgccgacaag ctttgatgtt actgcacggc cacaccgagg aaccgcctgg
     1681 actaagtctg gatttcctga agtgctgcaa gaggaatacg gccgaggaag agaactctgc
     1741 aaaccctaac caagaccaga acgcacgccg aagaaagaag aaggagagac gtcctagggg
     1801 caccatgcag gctatcaaca atgaaagaaa agcttcgaaa gtccttggga ttgttttctt
     1861 tgtgtttctg atcatgtggt gcccattttt cattaccaat attctgtctg ttctttgtga
     1921 gaagtcctgt aaccaaaagc tcatggaaaa gcttctgaat gtgtttgttt ggattggcta
     1981 tgtttgttca ggaatcaatc ctctggtgta tactctgttc aacaaaattt accgaagggc
     2041 attctccaac tatttgcgtt gcaattataa ggtagagaaa aagcctcctg tcaggcagat
     2101 tccaagagtt gccgccactg ctttgtctgg gagggagctt aatgttaaca tttatcggca
     2161 taccaatgaa ccggtgatcg agaaagccag tgacaatgag cccggtatag agatgcaagt
     2221 tgagaattta gagttaccag taaatccctc cagtgtggtt agcgaaagga ttagcagtgt
     2281 gggtggaggc ggatcaggag gcggtggatc tgagcccaaa tcttgtgaca aaactcacac
     2341 atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc
     2401 aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga
     2461 cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca
     2521 taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt
     2581 cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa
     2641 caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga
     2701 accacaggtg tacaccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct
     2761 gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg
     2821 gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt
     2881 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg
     2941 ctccgtgatg catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc
     3001 gggtaaatga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3061 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3121 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3181 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3241 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3301 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3361 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3421 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3481 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3541 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3601 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3661 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3721 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     3781 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3841 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3901 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3961 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4021 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4081 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     4141 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4201 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4261 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4321 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4381 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4441 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4501 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4561 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4621 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4681 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4741 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4801 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4861 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4921 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4981 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5041 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5101 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5281 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5341 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5401 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5461 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6121 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     6241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7441 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
