LOCUS       V031863                 8462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031863
VERSION     V031863
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3982
                     /label="IFIH1(NM_022168)"
                     /note="IFIH1(NM_022168)"
                     /gene="IFIH1"
     polyA_signal    4029..4253
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4299..4727
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4741..5070
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5137..5931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6105..6226
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6275..6291)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6299..6315
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6323..6353)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6368..6389
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6677..7262)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7433..8293)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8294..8398)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tcgaatgggt attccacaga cgagaatttc cgctatctca tctcgtgctt
      961 cagggccagg gtgaaaatgt acatccaggt ggagcctgtg ctggactacc tgacctttct
     1021 gcctgcagag gtgaaggagc agattcagag gacagtcgcc acctccggga acatgcaggc
     1081 agttgaactg ctgctgagca ccttggagaa gggagtctgg caccttggtt ggactcggga
     1141 attcgtggag gccctccgga gaaccggcag ccctctggcc gcccgctaca tgaaccctga
     1201 gctcacggac ttgccctctc catcgtttga gaacgctcat gatgaatatc tccaactgct
     1261 gaacctcctt cagcccactc tggtggacaa gcttctagtt agagacgtct tggataagtg
     1321 catggaggag gaactgttga caattgaaga cagaaaccgg attgctgctg cagaaaacaa
     1381 tggaaatgaa tcaggtgtaa gagagctact aaaaaggatt gtgcagaaag aaaactggtt
     1441 ctctgcattt ctgaatgttc ttcgtcaaac aggaaacaat gaacttgtcc aagagttaac
     1501 aggctctgat tgctcagaaa gcaatgcaga gattgagaat ttatcacaag ttgatggtcc
     1561 tcaagtggaa gagcaacttc tttcaaccac agttcagcca aatctggaga aggaggtctg
     1621 gggcatggag aataactcat cagaatcatc ttttgcagat tcttctgtag tttcagaatc
     1681 agacacaagt ttggcagaag gaagtgtcag ctgcttagat gaaagtcttg gacataacag
     1741 caacatgggc agtgattcag gcaccatggg aagtgattca gatgaagaga atgtggcagc
     1801 aagagcatcc ccggagccag aactccagct caggccttac caaatggaag ttgcccagcc
     1861 agccttggaa gggaagaata tcatcatctg cctccctaca gggagtggaa aaaccagagt
     1921 ggctgtttac attgccaagg atcacttaga caagaagaaa aaagcatctg agcctggaaa
     1981 agttatagtt cttgtcaata aggtactgct agttgaacag ctcttccgca aggagttcca
     2041 accatttttg aagaaatggt atcgtgttat tggattaagt ggtgataccc aactgaaaat
     2101 atcatttcca gaagttgtca agtcctgtga tattattatc agtacagctc aaatccttga
     2161 aaactccctc ttaaacttgg aaaatggaga agatgctggt gttcaattgt cagacttttc
     2221 cctcattatc attgatgaat gtcatcacac caacaaagaa gcagtgtata ataacatcat
     2281 gaggcattat ttgatgcaga agttgaaaaa caatagactc aagaaagaaa acaaaccagt
     2341 gattcccctt cctcagatac tgggactaac agcttcacct ggtgttggag gggccacgaa
     2401 gcaagccaaa gctgaagaac acattttaaa actatgtgcc aatcttgatg catttactat
     2461 taaaactgtt aaagaaaacc ttgatcaact gaaaaaccaa atacaggagc catgcaagaa
     2521 gtttgccatt gcagatgcaa ccagagaaga tccatttaaa gagaaacttc tagaaataat
     2581 gacaaggatt caaacttatt gtcaaatgag tccaatgtca gattttggaa ctcaacccta
     2641 tgaacaatgg gccattcaaa tggaaaaaaa agctgcaaaa gaaggaaatc gcaaagaacg
     2701 tgtttgtgca gaacatttga ggaagtacaa tgaggcccta caaattaatg acacaattcg
     2761 aatgatagat gcgtatactc atcttgaaac tttctataat gaagagaaag ataagaagtt
     2821 tgcagtcata gaagatgata gtgatgaggg tggtgatgat gagtattgtg atggtgatga
     2881 agatgaggat gatttaaaga aacctttgaa actggatgaa acagatagat ttctcatgac
     2941 tttatttttt gaaaacaata aaatgttgaa aaggctggct gaaaacccag aatatgaaaa
     3001 tgaaaagctg accaaattaa gaaataccat aatggagcaa tatactagga ctgaggaatc
     3061 agcacgagga ataatcttta caaaaacacg acagagtgca tatgcgcttt cccagtggat
     3121 tactgaaaat gaaaaatttg ctgaagtagg agtcaaagcc caccatctga ttggagctgg
     3181 acacagcagt gagttcaaac ccatgacaca gaatgaacaa aaagaagtca ttagtaaatt
     3241 tcgcactgga aaaataaatc tgcttatcgc taccacagtg gcagaagaag gtctggatat
     3301 taaagaatgt aacattgtta tccgttatgg tctcgtcacc aatgaaatag ccatggtcca
     3361 ggcccgtggt cgagccagag ctgatgagag cacctacgtc ctggttgctc acagtggttc
     3421 aggagttatc gaacatgaga cagttaatga tttccgagag aagatgatgt ataaagctat
     3481 acattgtgtt caaaatatga aaccagagga gtatgctcat aagattttgg aattacagat
     3541 gcaaagtata atggaaaaga aaatgaaaac caagagaaat attgccaagc attacaagaa
     3601 taacccatca ctaataactt tcctttgcaa aaactgcagt gtgctagcct gttctgggga
     3661 agatatccat gtaattgaga aaatgcatca cgtcaatatg accccagaat tcaaggaact
     3721 ttacattgta agagaaaaca aagcactgca aaagaagtgt gccgactatc aaataaatgg
     3781 tgaaatcatc tgcaaatgtg gccaggcttg gggaacaatg atggtgcaca aaggcttaga
     3841 tttgccttgt ctcaaaataa ggaattttgt agtggttttc aaaaataatt caacaaagaa
     3901 acaatacaaa aagtgggtag aattacctat cacatttccc aatcttgact attcagaatg
     3961 ctgtttattt agtgatgagg attagctcga gtctagaggg cccgtttaaa cccgctgatc
     4021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5101 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5161 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5221 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5281 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5341 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5401 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5461 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5521 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5581 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5641 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5701 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5761 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5821 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5881 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5941 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6001 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6061 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6241 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7201 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7741 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8041 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8401 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8461 gt
//
