LOCUS       V037465                10439 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037465
VERSION     V037465
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..5878
                     /label="IQGAP1(NM_003870)"
                     /note="IQGAP1(NM_003870)"
                     /gene="IQGAP1"
     CDS             5885..5911
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5915..5941
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5945..5971
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    6006..6230
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6276..6704
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6718..7047
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7114..7908
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8082..8203
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8252..8268)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8276..8292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8300..8330)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8345..8366
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8654..9239)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9410..10270)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10271..10375)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tccgccgcag acgaggttga cgggctgggc gtggcccggc cgcactatgg
      961 ctctgtcctg gataatgaaa gacttactgc agaggagatg gatgaaagga gacgtcagaa
     1021 cgtggcttat gagtaccttt gtcatttgga agaagcgaag aggtggatgg aagcatgcct
     1081 aggggaagat ctgcctccca ccacagaact ggaggagggg cttaggaatg gggtctacct
     1141 tgccaaactg gggaacttct tctctcccaa agtagtgtcc ctgaaaaaaa tctatgatcg
     1201 agaacagacc agatacaagg cgactggcct ccactttaga cacactgata atgtgattca
     1261 gtggttgaat gccatggatg agattggatt gcctaagatt ttttacccag aaactacaga
     1321 tatctatgat cgaaagaaca tgccaagatg tatctactgt atccatgcac tcagtttgta
     1381 cctgttcaag ctaggcctgg cccctcagat tcaagaccta tatggaaagg ttgacttcac
     1441 agaagaagaa atcaacaaca tgaagactga gttggagaag tatggcatcc agatgcctgc
     1501 ctttagcaag attgggggca tcttggctaa tgaactgtca gtggatgaag ccgcattaca
     1561 tgctgctgtt attgctatta atgaagctat tgaccgtaga attccagccg acacatttgc
     1621 agctttgaaa aatccgaatg ccatgcttgt aaatcttgaa gagcccttgg catccactta
     1681 ccaggatata ctttaccagg ctaagcagga caaaatgaca aatgctaaaa acaggacaga
     1741 aaactcagag agagaaagag atgtttatga ggagctgctc acgcaagctg aaattcaagg
     1801 caatataaac aaagtcaata cattttctgc attagcaaat atcgacctgg ctttagaaca
     1861 aggagatgca ctggccttgt tcagggctct gcagtcacca gccctggggc ttcgaggact
     1921 gcagcaacag aatagcgact ggtacttgaa gcagctcctg agtgataaac agcagaagag
     1981 acagagtggt cagactgacc ccctgcagaa ggaggagctg cagtctggag tggatgctgc
     2041 aaacagtgct gcccagcaat atcagagaag attggcagca gtagcactga ttaatgctgc
     2101 aatccagaag ggtgttgctg agaagactgt tttggaactg atgaatcccg aagcccagct
     2161 gccccaggtg tatccatttg ccgccgatct ctatcagaag gagctggcta ccctgcagcg
     2221 acaaagtcct gaacataatc tcacccaccc agagctctct gtcgcagtgg agatgttgtc
     2281 atcggtggcc ctgatcaaca gggcattgga atcaggagat gtgaatacag tgtggaagca
     2341 attgagcagt tcagttactg gtcttaccaa tattgaggaa gaaaactgtc agaggtatct
     2401 cgatgagttg atgaaactga aggctcaggc acatgcagag aataatgaat tcattacatg
     2461 gaatgatatc caagcttgcg tggaccatgt gaacctggtg gtgcaagagg aacatgagag
     2521 gattttagcc attggtttaa ttaatgaagc cctggatgaa ggtgatgccc aaaagactct
     2581 gcaggcccta cagattcctg cagctaaact tgagggagtc cttgcagaag tggcccagca
     2641 ttaccaagac acgctgatta gagcgaagag agagaaagcc caggaaatcc aggatgagtc
     2701 agctgtgtta tggttggatg aaattcaagg tggaatctgg cagtccaaca aagacaccca
     2761 agaagcacag aagtttgcct taggaatctt tgccattaat gaggcagtag aaagtggtga
     2821 tgttggcaaa acactgagtg cccttcgctc ccctgatgtt ggcttgtatg gagtcatccc
     2881 tgagtgtggt gaaacttacc acagtgatct tgctgaagcc aagaagaaaa aactggcagt
     2941 aggagataat aacagcaagt gggtgaagca ctgggtaaaa ggtggatatt attattacca
     3001 caatctggag acccaggaag gaggatggga tgaacctcca aattttgtgc aaaattctat
     3061 gcagctttct cgggaggaga tccagagttc tatctctggg gtgactgccg catataaccg
     3121 agaacagctg tggctggcca atgaaggcct gatcaccagg ctgcaggctc gctgccgtgg
     3181 atacttagtt cgacaggaat tccgatccag gatgaatttc ctgaagaaac aaatccctgc
     3241 catcacctgc attcagtcac agtggagagg atacaagcag aagaaggcat atcaagatcg
     3301 gttagcttac ctgcgctccc acaaagatga agttgtaaag attcagtccc tggcaaggat
     3361 gcaccaagct cgaaagcgct atcgagatcg cctgcagtac ttccgggacc atataaatga
     3421 cattatcaaa atccaggctt ttattcgggc aaacaaagct cgggatgact acaagactct
     3481 catcaatgct gaggatcctc ctatggttgt ggtccgaaaa tttgtccacc tgctggacca
     3541 aagtgaccag gattttcagg aggagcttga ccttatgaag atgcgggaag aggttatcac
     3601 cctcattcgt tctaaccagc agctggagaa tgacctcaat ctcatggata tcaaaattgg
     3661 actgctagtg aaaaataaga ttacgttgca ggatgtggtt tcccacagta aaaaacttac
     3721 caaaaaaaat aaggaacagt tgtctgatat gatgatgata aataaacaga agggaggtct
     3781 caaggctttg agcaaggaga agagagagaa gttggaagct taccagcacc tgttttattt
     3841 attgcaaacc aatcccacct atctggccaa gctcattttt cagatgcccc agaacaagtc
     3901 caccaagttc atggactctg taatcttcac actctacaac tacgcgtcca accagcgaga
     3961 ggagtacctg ctcctgcggc tctttaagac agcactccaa gaggaaatca agtcgaaggt
     4021 agatcagatt caagagattg tgacaggaaa tcctacggtt attaaaatgg ttgtaagttt
     4081 caaccgtggt gcccgtggcc agaatgccct gagacagatc ttggccccag tcgtgaagga
     4141 aattatggat gacaaatctc tcaacatcaa aactgaccct gtggatattt acaaatcttg
     4201 ggttaatcag atggagtctc agacaggaga ggcaagcaaa ctgccctatg atgtgacccc
     4261 tgagcaggcg ctagctcatg aagaagtgaa gacacggcta gacagctcca tcaggaacat
     4321 gcgggctgtg acagacaagt ttctctcagc cattgtcagc tctgtggaca aaatccctta
     4381 tgggatgcgc ttcattgcca aagtgctgaa ggactcgttg catgagaagt tccctgatgc
     4441 tggtgaggat gagctgctga agattattgg taacttgctt tattatcgat acatgaatcc
     4501 agccattgtt gctcctgatg cctttgacat cattgacctg tcagcaggag gccagcttac
     4561 cacagaccaa cgccgaaatc tgggctccat tgcaaaaatg cttcagcatg ctgcttccaa
     4621 taagatgttt ctgggagata atgcccactt aagcatcatt aatgaatatc tttcccagtc
     4681 ctaccagaaa ttcagacggt ttttccaaac tgcttgtgat gtcccagagc ttcaggataa
     4741 atttaatgtg gatgagtact ctgatttagt aaccctcacc aaaccagtaa tctacatttc
     4801 cattggtgaa atcatcaaca cccacactct cctgttggat caccaggatg ccattgctcc
     4861 ggagcacaat gatccaatcc acgaactgct ggacgacctc ggcgaggtgc ccaccatcga
     4921 gtccctgata ggggaaagct ctggcaattt aaatgaccca aataaggagg cactggctaa
     4981 gacggaagtg tctctcaccc tgaccaacaa gttcgacgtg cctggagatg agaatgcaga
     5041 aatggatgct cgaaccatct tactgaatac aaaacgttta attgtggatg tcatccggtt
     5101 ccagccagga gagaccttga ctgaaatcct agaaacacca gccaccagtg aacaggaagc
     5161 agaacatcag agagccatgc agagacgtgc tatccgtgat gccaaaacac ctgacaagat
     5221 gaaaaagtca aaatctgtaa aggaagacag caacctcact cttcaagaga agaaagagaa
     5281 gatccagaca ggtttaaaga agctaacaga gcttggaacc gtggacccaa agaacaaata
     5341 ccaggaactg atcaacgaca ttgccaggga tattcggaat cagcggaggt accgacagag
     5401 gagaaaggcc gaactagtga aactgcaaca gacatacgct gctctgaact ctaaggccac
     5461 cttttatggg gagcaggtgg attactataa aagctatatc aaaacctgct tggataactt
     5521 agccagcaag ggcaaagtct ccaaaaagcc tagggaaatg aaaggaaaga aaagcaaaaa
     5581 gatttctctg aaatatacag cagcaagact acatgaaaaa ggagttcttc tggaaattga
     5641 ggacctgcaa gtgaatcagt ttaaaaatgt tatatttgaa atcagtccaa cagaagaagt
     5701 tggagacttc gaagtgaaag ccaaattcat gggagttcaa atggagactt ttatgttaca
     5761 ttatcaggac ctgctgcagc tacagtatga aggagttgca gtcatgaaat tatttgatag
     5821 agctaaagta aatgtcaacc tcctgatctt ccttctcaac aaaaagttct acgggaagct
     5881 cgagtaccca tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc
     5941 tagctatcct tatgatgtcc cagactacgc atgagggccc gtttaaaccc gctgatcagc
     6001 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6061 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6121 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6181 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6241 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6301 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6361 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6421 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6481 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6541 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6601 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6661 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6721 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6781 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6841 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6901 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6961 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7021 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7081 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     7141 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7201 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7261 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7321 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7381 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7441 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7501 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7561 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7621 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7681 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7741 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7801 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7861 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7921 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8221 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9181 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9721 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10021 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10381 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
