LOCUS V018403 7682 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018403 VERSION V018403 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 896..3202 /label="ITGB2(NM_000211)" /note="ITGB2(NM_000211)" /gene="ITGB2" polyA_signal 3249..3473 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3519..3947 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3961..4290 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4357..5151 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5325..5446 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5495..5511) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5519..5535 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5543..5573) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5588..5609 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5897..6482) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6653..7513) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7514..7618) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgct 901 gggcctgcgc cccccactgc tcgccctggt ggggctgctc tccctcgggt gcgtcctctc 961 tcaggagtgc acgaagttca aggtcagcag ctgccgggaa tgcatcgagt cggggcccgg 1021 ctgcacctgg tgccagaagc tgaacttcac agggccgggg gatcctgact ccattcgctg 1081 cgacacccgg ccacagctgc tcatgagggg ctgtgcggct gacgacatca tggaccccac 1141 aagcctcgct gaaacccagg aagaccacaa tgggggccag aagcagctgt ccccacaaaa 1201 agtgacgctt tacctgcgac caggccaggc agcagcgttc aacgtgacct tccggcgggc 1261 caagggctac cccatcgacc tgtactatct gatggacctc tcctactcca tgcttgatga 1321 cctcaggaat gtcaagaagc taggtggcga cctgctccgg gccctcaacg agatcaccga 1381 gtccggccgc attggcttcg ggtccttcgt ggacaagacc gtgctgccgt tcgtgaacac 1441 gcaccctgat aagctgcgaa acccatgccc caacaaggag aaagagtgcc agcccccgtt 1501 tgccttcagg cacgtgctga agctgaccaa caactccaac cagtttcaga ccgaggtcgg 1561 gaagcagctg atttccggaa acctggatgc acccgagggt gggctggacg ccatgatgca 1621 ggtcgccgcc tgcccggagg aaatcggctg gcgcaacgtc acgcggctgc tggtgtttgc 1681 cactgatgac ggcttccatt tcgcgggcga cgggaagctg ggcgccatcc tgacccccaa 1741 cgacggccgc tgtcacctgg aggacaactt gtacaagagg agcaacgaat tcgactaccc 1801 atcggtgggc cagctggcgc acaagctggc tgaaaacaac atccagccca tcttcgcggt 1861 gaccagtagg atggtgaaga cctacgagaa actcaccgag atcatcccca agtcagccgt 1921 gggggagctg tctgaggact ccagcaatgt ggtccatctc attaagaatg cttacaataa 1981 actctcctcc agggtcttcc tggatcacaa cgccctcccc gacaccctga aagtcaccta 2041 cgactccttc tgcagcaatg gagtgacgca caggaaccag cccagaggtg actgtgatgg 2101 cgtgcagatc aatgtcccga tcaccttcca ggtgaaggtc acggccacag agtgcatcca 2161 ggagcagtcg tttgtcatcc gggcgctggg cttcacggac atagtgaccg tgcaggttct 2221 tccccagtgt gagtgccggt gccgggacca gagcagagac cgcagcctct gccatggcaa 2281 gggcttcttg gagtgcggca tctgcaggtg tgacactggc tacattggga aaaactgtga 2341 gtgccagaca cagggccgga gcagccagga gctggaagga agctgccgga aggacaacaa 2401 ctccatcatc tgctcagggc tgggggactg tgtctgcggg cagtgcctgt gccacaccag 2461 cgacgtcccc ggcaagctga tatacgggca gtactgcgag tgtgacacca tcaactgtga 2521 gcgctacaac ggccaggtct gcggcggccc ggggaggggg ctctgcttct gcgggaagtg 2581 ccgctgccac ccgggctttg agggctcagc gtgccagtgc gagaggacca ctgagggctg 2641 cctgaacccg cggcgtgttg agtgtagtgg tcgtggccgg tgccgctgca acgtatgcga 2701 gtgccattca ggctaccagc tgcctctgtg ccaggagtgc cccggctgcc cctcaccctg 2761 tggcaagtac atctcctgcg ccgagtgcct gaagttcgaa aagggcccct ttgggaagaa 2821 ctgcagcgcg gcgtgtccgg gcctgcagct gtcgaacaac cccgtgaagg gcaggacctg 2881 caaggagagg gactcagagg gctgctgggt ggcctacacg ctggagcagc aggacgggat 2941 ggaccgctac ctcatctatg tggatgagag ccgagagtgt gtggcaggcc ccaacatcgc 3001 cgccatcgtc gggggcaccg tggcaggcat cgtgctgatc ggcattctcc tgctggtcat 3061 ctggaaggct ctgatccacc tgagcgacct ccgggagtac aggcgctttg agaaggagaa 3121 gctcaagtcc cagtggaaca atgataatcc ccttttcaag agcgccacca cgacggtcat 3181 gaaccccaag tttgctgaga gttagctcga gtctagaggg cccgtttaaa cccgctgatc 3241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 3301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 3361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 3421 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 3481 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 3541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 3601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 3661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 3721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 3781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 3841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 3901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 3961 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4021 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 4081 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 4141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 4201 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 4261 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 4321 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 4381 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 4441 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 4501 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 4561 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 4621 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 4681 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 4741 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 4801 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 4861 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 4921 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4981 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 5041 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 5101 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 5161 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 5221 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 5281 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 5341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 5401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 5461 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 5521 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 5581 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 5641 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 5701 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 5761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 5821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 5881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 6121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 6181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 6241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 6301 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 6361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 6421 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 6601 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 6661 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 6721 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 6781 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 6841 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 6901 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 6961 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7021 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7081 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7141 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7201 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7261 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7321 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7381 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7441 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7501 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7561 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 7621 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 7681 gt //