LOCUS       V023878                 9591 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023878
VERSION     V023878
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1660..5055
                     /label="JAK2(NM_004972)"
                     /note="JAK2(NM_004972)"
                     /gene="JAK2"
     polyA_signal    5158..5382
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5428..5856
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5870..6199
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6266..7060
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7234..7355
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7404..7420)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7428..7444
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7452..7482)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7497..7518
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7806..8391)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8562..9422)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9423..9527)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg cggcggaggg
     1621 agtggaggag ggggaagcgg aggcggaggg agtaagctta tgggaatggc ctgccttacg
     1681 atgacagaaa tggagggaac atccacctct tctatatatc agaatggtga tatttctgga
     1741 aatgccaatt ctatgaagca aatagatcca gttcttcagg tgtatcttta ccattccctt
     1801 gggaaatctg aggcagatta tctgaccttt ccatctgggg agtatgttgc agaagaaatc
     1861 tgtattgctg cttctaaagc ttgtggtatc acacctgtgt atcataatat gtttgcttta
     1921 atgagtgaaa cagaaaggat ctggtatcca cccaaccatg tcttccatat agatgagtca
     1981 accaggcata atgtactcta cagaataaga ttttactttc ctcgttggta ttgcagtggc
     2041 agcaacagag cctatcggca tggaatatct cgaggtgctg aagctcctct tcttgatgac
     2101 tttgtcatgt cttacctctt tgctcagtgg cggcatgatt ttgtgcacgg atggataaaa
     2161 gtacctgtga ctcatgaaac acaggaagaa tgtcttggga tggcagtgtt agatatgatg
     2221 agaatagcca aagaaaacga tcaaacccca ctggccatct ataactctat cagctacaag
     2281 acattcttac caaaatgtat tcgagcaaag atccaagact atcatatttt gacaaggaag
     2341 cgaataaggt acagatttcg cagatttatt cagcaattca gccaatgcaa agccactgcc
     2401 agaaacttga aacttaagta tcttataaat ctggaaactc tgcagtctgc cttctacaca
     2461 gagaaatttg aagtaaaaga acctggaagt ggtccttcag gtgaggagat ttttgcaacc
     2521 attataataa ctggaaacgg tggaattcag tggtcaagag ggaaacataa agaaagtgag
     2581 acactgacag aacaggattt acagttatat tgcgattttc ctaatattat tgatgtcagt
     2641 attaagcaag caaaccaaga gggttcaaat gaaagccgag ttgtaactat ccataagcaa
     2701 gatggtaaaa atctggaaat tgaacttagc tcattaaggg aagctttgtc tttcgtgtca
     2761 ttaattgatg gatattatag attaactgca gatgcacatc attacctctg taaagaagta
     2821 gcacctccag ccgtgcttga aaatatacaa agcaactgtc atggcccaat ttcgatggat
     2881 tttgccatta gtaaactgaa gaaagcaggt aatcagactg gactgtatgt acttcgatgc
     2941 agtcctaagg actttaataa atattttttg acttttgctg tcgagcgaga aaatgtcatt
     3001 gaatataaac actgtttgat tacaaaaaat gagaatgaag agtacaacct cagtgggaca
     3061 aagaagaact tcagcagtct taaagatctt ttgaattgtt accagatgga aactgttcgc
     3121 tcagacaata taattttcca gtttactaaa tgctgtcccc caaagccaaa agataaatca
     3181 aaccttctag tcttcagaac gaatggtgtt tctgatgtac caacctcacc aacattacag
     3241 aggcctactc atatgaacca aatggtgttt cacaaaatca gaaatgaaga tttgatattt
     3301 aatgaaagcc ttggccaagg cacttttaca aagattttta aaggcgtacg aagagaagta
     3361 ggagactacg gtcaactgca tgaaacagaa gttcttttaa aagttctgga taaagcacac
     3421 agaaactatt cagagtcttt ctttgaagca gcaagtatga tgagcaagct ttctcacaag
     3481 catttggttt taaattatgg agtatgtgtc tgtggagacg agaatattct ggttcaggag
     3541 tttgtaaaat ttggatcact agatacatat ctgaaaaaga ataaaaattg tataaatata
     3601 ttatggaaac ttgaagttgc taaacagttg gcatgggcca tgcattttct agaagaaaac
     3661 acccttattc atgggaatgt atgtgccaaa aatattctgc ttatcagaga agaagacagg
     3721 aagacaggaa atcctccttt catcaaactt agtgatcctg gcattagtat tacagttttg
     3781 ccaaaggaca ttcttcagga gagaatacca tgggtaccac ctgaatgcat tgaaaatcct
     3841 aaaaatttaa atttggcaac agacaaatgg agttttggta ccactttgtg ggaaatctgc
     3901 agtggaggag ataaacctct aagtgctctg gattctcaaa gaaagctaca attttatgaa
     3961 gataggcatc agcttcctgc accaaagtgg gcagaattag caaaccttat aaataattgt
     4021 atggattatg aaccagattt caggccttct ttcagagcca tcatacgaga tcttaacagt
     4081 ttgtttactc cagattatga actattaaca gaaaatgaca tgttaccaaa tatgaggata
     4141 ggtgccctgg ggttttctgg tgcctttgaa gaccgggatc ctacacagtt tgaagagaga
     4201 catttgaaat ttctacagca acttggcaag ggtaattttg ggagtgtgga gatgtgccgg
     4261 tatgaccctc tacaggacaa cactggggag gtggtcgctg taaaaaagct tcagcatagt
     4321 actgaagagc acctaagaga ctttgaaagg gaaattgaaa tcctgaaatc cctacagcat
     4381 gacaacattg taaagtacaa gggagtgtgc tacagtgctg gtcggcgtaa tctaaaatta
     4441 attatggaat atttaccata tggaagttta cgagactatc ttcaaaaaca taaagaacgg
     4501 atagatcaca taaaacttct gcagtacaca tctcagatat gcaagggtat ggagtatctt
     4561 ggtacaaaaa ggtatatcca cagggatctg gcaacgagaa atatattggt ggagaacgag
     4621 aacagagtta aaattggaga ttttgggtta accaaagtct tgccacaaga caaagaatac
     4681 tataaagtaa aagaacctgg tgaaagtccc atattctggt atgctccaga atcactgaca
     4741 gagagcaagt tttctgtggc ctcagatgtt tggagctttg gagtggttct gtatgaactt
     4801 ttcacataca ttgagaagag taaaagtcca ccagcggaat ttatgcgtat gattggcaat
     4861 gacaaacaag gacagatgat cgtgttccat ttgatagaac ttttgaagaa taatggaaga
     4921 ttaccaagac cagatggatg cccagatgag atctatatga tcatgacaga atgctggaac
     4981 aataatgtaa atcaacgccc ctcctttagg gatctagctc ttcgagtgga tcaaataagg
     5041 gataacatgg ctggatgagg atccactagt ccagtgtggt ggaattctgc agatatccag
     5101 cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     5161 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5221 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5281 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5341 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5401 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5461 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5521 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5581 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5641 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5701 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5761 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5821 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5881 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5941 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6001 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6121 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6181 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6241 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6301 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6361 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6421 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6481 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6541 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6601 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6661 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6721 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6781 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6841 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6901 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6961 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7021 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7081 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7201 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7381 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8461 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8521 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8581 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8641 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8701 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8761 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8821 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8881 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8941 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9001 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9061 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9121 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9181 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9241 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9301 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9361 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9421 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9481 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9541 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg t
//
