LOCUS       V038101                 9547 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038101
VERSION     V038101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1642..5037
                     /label="Jak2(NM_031514)"
                     /note="Jak2(NM_031514)"
                     /gene="Jak2"
     polyA_signal    5114..5338
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5384..5812
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5826..6155
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6222..7016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7190..7311
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7360..7376)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7384..7400
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7408..7438)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7453..7474
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7762..8347)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8518..9378)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9379..9483)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc
     1621 ggaggcggag ggagtgaatt catgggaatg gcttgcctta caatgacaga aatggaggga
     1681 acctccacat ctcctgcaca tcagaatggt gatattcctg gaaatgctaa ctctgtgaag
     1741 cagacagagc cagtccttca agtgtacctg taccattctc ttgggcaagc tgaaggagac
     1801 tatctgaagt ttccaaatgg agagtatgtt gccgaagaaa tttgtgtggc tgcttctaaa
     1861 gcatgtggta ttacgcctgt gtatcataat atgtttgctt taatgagtga aacagaaagg
     1921 atctggtatc cacccaatca tgtcttccac atagatgagt caaccaggca taatatactc
     1981 tacagaataa ggttttactt tcctcattgg tactgcagtg gcagcaacag aacctatagg
     2041 tacggagtat ctcgtggggc tgaagctcct ctccttgacg actttgtcat gtcttacctt
     2101 tttgctcagt ggcggcatga ttttgttcat gggtggataa aagtacctgt gactcacgaa
     2161 actcaggaag aatgtcttgg catggcagtg ttagacatga tgagaatagc taaggagaag
     2221 gaccagactc ccctggctgt ctataactcc atcagctaca agacgttctt accgaagtgc
     2281 gtgcgagcga agatccagga ctatcacatt ttaactcgga agcggataag gtacagattc
     2341 cgcaggttca ttcagcagtt cagtcagtgc aaagccactg ccaggaacct aaaacttaag
     2401 tatctcataa acctggagac cctgcagtct gccttctaca cagaacagtt tgaagtaaaa
     2461 gaatctgcaa gaggtccttc aggtgaggag atttttgcaa ccattataat aactggaaac
     2521 ggtggaattc agtggtcaag agggaaacat aaggaaagtg agacactgac agaacaggat
     2581 ttacagttgt attgtgattt tcctgatatt attgatgtca gtattaagca agcaaatcag
     2641 gaatgctcaa ctgaaagtag agttgtgacc gtccacaagc aggacgggaa ggtcttggaa
     2701 atagaactta gctcattaaa agaagccttg tcatttgtgt cgttaattga cgggtattac
     2761 agactaactg cggatgcaca ccattacctc tgtaaagagg tggctcctcc agccgtgctt
     2821 gaaaacatac acagcaactg tcacggccca atttcaatgg attttgccat cagcaaacta
     2881 aagaaggcag gaaaccagac tggactgtat gtacttcgat gtagccctaa ggacttcaac
     2941 aaatacttcc tgacctttgc cgttgagcga gaaaatgtta ttgaatataa acactgtttg
     3001 attacaaaga atgagaatgg agagtacaac ctcagtggga ctaagaggaa cttcagtagt
     3061 cttaaggacc ttttgaattg ctaccagatg gaaactgtgc gctcagacag tatcatcttc
     3121 cagttcacca aatgctgtcc tccaaagccg aaagataaat caaaccttct tgtcttcaga
     3181 acaaatggtg tttctgatgt tcagctctca ccaacattac agaggcataa taatgtgaat
     3241 caaatggtgt ttcacaaaat caggaatgaa gatttgatat ttaatgaaag ccttggccaa
     3301 ggcactttta caaaaatatt taaaggtgta agaagagaag ttggagatta tggtcagctg
     3361 cacgaaaccg aagttctttt gaaagtccta gataaagcac atagaaacta ttcagagtct
     3421 ttctttgaag cagcaagcat gatgagtcag ctttctcaca agcatttggt tttgaattat
     3481 ggagtatgtg tctgtggaga ggagaacatt ttggttcaag agtttgtaaa atttggatcc
     3541 ctggatacat acctgaaaaa aaacaaaaat tccataaata tactatggaa acttggagtg
     3601 gccaaacagt tggcatgggc catgcacttc ctcgaagaaa aatcccttat tcatgggaat
     3661 gtgtgtgcca aaaatatcct gcttatcaga gaagaagaca ggaaaacagg gaatccacct
     3721 ttcatcaaac ttagtgatcc tgggattagc atcacagttc taccaaagga cattcttcag
     3781 gagagaatac catgggtacc acctgagtgc attgaaaacc ctaaaaatct aactttggca
     3841 acagacaagt ggagctttgg caccactctg tgggagatct gcagtggagg agacaagcct
     3901 ctgagtgctc tggactctca aagaaagctg cagttctatg aagataagca ccagctccca
     3961 gcacccaagt ggacagagtt ggcaaacctt ataaatactt gcatggatta tgagccagac
     4021 ttcaggcctg ccttcagagc tgtcatccgt gatctgaaca gcctgtttac tccagattat
     4081 gaactgctga cagaaaatga catgctaccc aacatgagaa taggtgccct aggattttct
     4141 ggtgcttttg aagacaggga ccctacacag tttgaagaga gacacttgaa atttctacag
     4201 caacttggca agggtaactt tgggagtgtg gagatgtgcc gctatgaccc gctgcaggac
     4261 aacactggag aggtggtagc tgttaagaag ctccagcaca gcacggaaga gcatctccga
     4321 gactttgaaa gggaaattga aatcctgaaa tccttgcagc atgacaacat cgtgaagtac
     4381 aagggagtgt gctacagtgc tggtcggcgc aacctaagat taattatgga atatttgccg
     4441 tatggaagtt tacgagacta tctccaaaaa cataaagaac ggatagatca caaaaaactt
     4501 ctgcagtata catcccagat atgcaagggc atggagtatc ttggtacaaa aaggtatatc
     4561 caccgggatc tggcaacaag gaatatattg gtagaaaatg agaacagagt taaaatagga
     4621 gattttggat taaccaaagt cttgccacag gacaaagaat actacaaagt aaaggaacca
     4681 ggtgaaagtc ccatattctg gtatgctccc gaatccttga cagagagcaa gttttctgtg
     4741 gcctcagatg tgtggagctt tggtgtggtt ctgtacgaac ttttcacgta tatcgagaag
     4801 agcaaaagcc cacctgtgga atttatgcga atgatcggca atgataaaca agggcagatg
     4861 atcgtattcc atttgataga gctactgaag aacaacggaa gactgccgag accagaaggg
     4921 tgcccagacg agatttatgt gatcatgaca gaatgctgga acaacaatgt caaccaacgt
     4981 ccctctttca gggacctttc ccttcgggtg gatcaaatcc gggacagtat ggctgcatga
     5041 ggatcctgat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     5101 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5161 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5221 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5281 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5341 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5401 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5461 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5521 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5581 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5641 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5701 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5761 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5821 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5881 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6001 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6061 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6181 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6241 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6301 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6361 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6421 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6481 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6541 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6601 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6661 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6721 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6781 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6841 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6901 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6961 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7021 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7081 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7141 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7321 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7381 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7441 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7501 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7561 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8161 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8281 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8341 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8401 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8641 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9481 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     9541 ctatggt
//
