LOCUS       V042050                13636 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042050
VERSION     V042050
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..9075
                     /label="KMT2B(NM_014727)"
                     /note="KMT2B(NM_014727)"
                     /gene="KMT2B"
     CDS             9082..9108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9112..9138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9142..9168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    9203..9427
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9473..9901
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9915..10244
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10311..11105
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11279..11400
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11449..11465)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11473..11489
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11497..11527)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11542..11563
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11851..12436)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12607..13467)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13468..13572)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcggcgg cggcgggcgg cggcagttgc
      961 cccgggcctg gctccgcgcg gggccgcttc ccgggccggc cgcggggcgc cggcgggggc
     1021 gggggccgcg gcggacgggg caacggggcc gaaagagtgc gggtagctct gcggcgcggc
     1081 ggtggcgcga cggggccggg cggagccgag cccggggagg acacggccct gctccgtttg
     1141 ctggggctcc gccggggcct gcgccggctc cgccgcctgt gggccggccc gcgggtccag
     1201 cggggccggg gacggggtcg gggccggggc tggggcccga gtcgaggctg cgtgccggag
     1261 gaggagagca gtgacgggga atccgacgag gaggagtttc agggttttca ttcagatgaa
     1321 gatgtggccc ccagttccct gcgctctgcg ctccgatccc agcgaggtcg agcgccccga
     1381 ggtcggggtc gcaagcataa gacgaccccc cttcctcctc ctcgcctagc agatgtggct
     1441 cctacccccc caaagacccc tgcccggaaa cggggtgagg aaggcacaga acggatggtg
     1501 caggcactga ctgaacttct ccggcgggcc caggcacccc aagcaccccg gagccgggca
     1561 tgtgagccct ccaccccccg gcggtctcgg ggacggcccc caggacggcc agcaggcccc
     1621 tgcaggagga agcagcaagc agtagtggtg gcagaagcag ctgtgacaat ccccaaacct
     1681 gagcccccac ctcctgtggt tccagtgaaa catcagactg gcagctggaa atgcaaggag
     1741 gggcccggtc caggacctgg gacccccagg cgtggaggac agtcaagccg tggaggccgt
     1801 ggaggcaggg gccgcggccg aggtggtggg ctcccctttg tgatcaagtt tgtttcaagg
     1861 gccaaaaaag taaagatggg acaattgtcc ttgggactcg aatcaggtca aggtcaaggt
     1921 caacatgagg aaagttggca ggatgtcccc caaagaagag ttggatctgg acagggaggg
     1981 agcccttgct ggaaaaagca ggaacagaag ctggatgacg aggaagaaga gaagaaagaa
     2041 gaagaagaaa aagacaagga gggagaagag aaggaagaaa gagctgtagc tgaggagatg
     2101 atgccagctg cggaaaagga agaggcaaag ctgccaccac cgcctctgac tcctccagcc
     2161 ccttcacctc ctccacccct cccaccccct tcgacatctc ctccaccccc actctgccct
     2221 ccaccaccac ccccagtgtc cccaccacct ctaccatccc ctccaccgcc tcctgcccaa
     2281 gaggagcagg aggaatcccc tcctcctgtg gtcccagcta cgtgctccag gaagaggggc
     2341 cggcctcccc tgactcccag ccagcgggcg gagcgggaag ctgctcgggc agggccagag
     2401 ggcacctctc ctcccactcc aacccccagc accgccacgg gaggccctcc ggaagacagt
     2461 cccaccgtgg cccccaaaag caccaccttc ctgaagaata tccggcagtt tattatgcct
     2521 gtggtgagtg cccgctcctc ccgtgtcatc aagacacccc ggcgatttat ggatgaagac
     2581 ccccccaaac ccccaaaggt ggaggtctca cctgtcctgc gacctcccat taccacctcc
     2641 ccacctgttc cccaggagcc agcaccagtc ccctctccac cacgtgcccc aactcctcca
     2701 tctaccccag ttccactccc tgagaagaga cggtccatcc taagggaacc cacatttcgc
     2761 tggacctcac tgacccggga gctgccccct cctcccccag cccctccacc tcccccggcc
     2821 ccctccccac cccctgctcc tgccacctcc tcccggaggc ccctactcct tcgggcccct
     2881 cagtttaccc caagcgaagc ccacctgaag atctacgaat cggtgcttac tcctcctcct
     2941 cttggggctc ctgaagcccc tgagccagag cctcctcctg ccgatgactc tccagctgag
     3001 cctgagcctc gggcagtggg ccgcaccaac cacctcagcc tgcctcgatt cgcccctgtg
     3061 gtcaccactc ctgttaaggc cgaggtgtcc cctcacgggg ctccagctct gagcaacggg
     3121 ccacagacac aggctcagct actgcagccc ctgcaggcct tgcaaaccca gctcctgccc
     3181 caggcactac cgccaccaca gccacagctg cagccaccgc cgtcaccaca gcagatgcct
     3241 cccctggaaa aagcccggat tgcgggcgtg ggttccttgc cgctgtctgg ggtagaggag
     3301 aagatgttca gcctcctcaa gagagccaaa gtgcagctat tcaagatcga tcagcagcag
     3361 cagcagaagg tggcagcttc catgccgctg agccctggag ggcagatgga ggaggtggcc
     3421 ggggctgtca agcagatctc cgacagaggc cctgtccggt ctgaagatga gtcggtggaa
     3481 gctaagagag agcggccctc aggtcccgag tcccctgtgc aaggtccccg catcaaacat
     3541 gtctgccgtc atgctgctgt ggccctgggt caggcccggg ccatggtgcc tgaagatgtc
     3601 cctcgcctca gtgccctccc tctccgggat cggcaggacc tcgccacaga ggatacatca
     3661 tcggcgtccg agactgagag tgtcccgtca cggtcccggc ggggaaaggt ggaggcagca
     3721 ggccctgggg gagaatcaga gcccacaggt tctggaggga ccctggccca cacaccccgg
     3781 cgctcactgc cctcccatca cggcaagaag atgcgcatgg ctcgatgtgg acactgtcgg
     3841 ggctgcctac gtgtgcagga ctgtgggtcc tgtgtcaact gcctagacaa gcccaagttt
     3901 gggggcccta acaccaagaa gcagtgctgt gtataccgga agtgtgacaa aatagaggct
     3961 cggaagatgg aacgactggc taaaaaaggc cggacgatag tgaagacgct gttgccctgg
     4021 gattccgatg aatctcctga ggcctcccct ggtcctccag gcccacgccg gggggcggga
     4081 gctggggggc cccgggagga ggtggtggcc cacccagggc ccgaggagca ggactccctc
     4141 ctgcagcgca agtcagctcg gcgctgcgtc aaacagcgac cctcctatga tatcttcgag
     4201 gattcggatg actcggagcc cgggggcccc cctgctcctc ggcgtcggac cccccgagaa
     4261 aatgagctgc cactgccaga acctgaggag cagagccggc cccgcaaacc taccctgcag
     4321 cctgtgttgc agctcaaggc ccgaaggcgc ctggacaagg atgctttggc ccctggcccc
     4381 tttgcttctt ttcccaatgg ctggactgga aagcagaagt ctcccgatgg tgtgcaccgc
     4441 gtccgtgtgg attttaagga ggattgtgat ttagagaacg tgtggctgat ggggggcctg
     4501 agtgtgctca cctctgtgcc agggggcccc ccgatggtgt gcttgctgtg tgccagcaaa
     4561 ggactccacg agctggtgtt ctgtcaagtc tgctgtgacc cattccaccc attctgcctg
     4621 gaggaggccg agcggcccct gccccagcat cacgacacct ggtgctgccg tcgctgcaaa
     4681 ttctgccacg tctgtggacg caaaggtcgt ggatccaagc acctcctgga gtgcgagcgc
     4741 tgccgccatg cataccaccc ggcctgtctg gggcccagct atccaacccg ggccacgcgc
     4801 aaacggcgcc actggatctg ttcagcctgt gtgcgctgta agagctgtgg ggcaactcca
     4861 ggcaagaact gggacgtcga gtggtctgga gattacagcc tctgccccag gtgcacccag
     4921 ctatatgaga aaggaaacta ctgcccgatc tgtacacgct gctatgaaga caacgactat
     4981 gagagcaaga tgatgcagtg cgcacagtgc gatcactggg tgcatgccaa gtgcgagggg
     5041 ctctcagatg aagactacga gatcctttca ggactgccag actcggtgct gtacacctgc
     5101 ggaccgtgtg ctggggcagc gcagccccgc tggcgagagg ccctgagcgg ggccctccag
     5161 gggggcctgc gccaggtgct ccagggcctg ctgagctcca aggtggtggg cccactgctg
     5221 ctctgcaccc agtgtgggcc agatgggaag caactgcacc caggaccctg cggcctgcaa
     5281 gctgtgagtc agcgcttcga ggatggccac tacaagtctg tgcacagctt catggaggac
     5341 atggtgggca tcctcatgcg gcactcggag gagggagaga ccccggaccg ccgggctgga
     5401 ggccagatga aggggctcct gctgaagctg ctagaatctg cgttcggctg gttcgacgcc
     5461 cacgacccca agtactggcg acggagtacc cggctgccaa acggagtcct tcccaatgcg
     5521 gtgttgcccc catccctgga tcatgtctat gcgcagtgga gacagcagga accagagacc
     5581 ccagaatcag ggcagcctcc aggggatccc tcagcagcat tccagggcaa ggatccggct
     5641 gccttctcac acctggagga cccccgtcag tgtgcactct gcctcaaata cggggatgca
     5701 gactccaagg aggcggggcg gctcttgtac atcgggcaga acgagtggac acacgtcaac
     5761 tgtgccatct ggtcggcgga agtcttcgag gagaacgacg gctccctcaa gaatgtgcat
     5821 gctgctgtgg cccgagggag gcagatgcgc tgcgagctct gcctgaagcc tggcgccacg
     5881 gtgggctgct gcctgtcctc ctgcctcagc aacttccact tcatgtgtgc ccgggccagc
     5941 tactgcatct tccaggatga caagaaagtc ttctgccaga aacacactga tctcctggat
     6001 ggcaaggaaa ttgtgaaccc cgatggtttt gatgttctcc gccgagtcta tgtggacttc
     6061 gagggcatca acttcaagcg gaagttcttg acggggcttg aacccgatgc catcaacgtg
     6121 ctcattggtt ccatccgcat tgactccctg ggtactctgt ctgatctctc ggactgcgag
     6181 ggacggctct tccccattgg ctaccagtgc tcccgtctgt actggagcac agtggatgct
     6241 cggaggcgct gctggtatcg gtgccgaatt ctggagtatc ggccatgggg gccgagggaa
     6301 gagccagctc acctggaggc tgcagaggag aaccagacca ttgtgcacag ccccgcccct
     6361 tcctcagagc ccccaggtgg tgaggacccc ccactggaca cagatgttct tgtccctgga
     6421 gctcctgagc gccactcgcc cattcagaac ctggaccctc cactgcggcc agattcaggc
     6481 agcgcccctc ctccagcccc ccgttctttt tcgggggctc gaatcaaagt gcccaactac
     6541 tcgccatccc ggaggccctt ggggggtgtc tcctttggcc ccctgccctc ccctggaagt
     6601 ccatcttcac tgacccacca catccccaca gtgggagacc cggacttccc agctcccccc
     6661 agacgttccc gtcgtcccag ccctttggct cccaggccgc ctccatcacg gtgggcctcc
     6721 cctcctctaa aaacctcccc tcagctcagg gtgccccctc ctacctcagt cgtcacagcc
     6781 ctcacaccta cctcagggga gctggctccc cctggcccgg ccccatctcc accaccccct
     6841 gaagacctgg gcccagactt cgaggacatg gaggtggtgt caggactgag tgctgctgac
     6901 ctggacttcg cggccagcct gctggggact gagcccttcc aggaagagat tgtagccgct
     6961 ggggccatgg ggagcagcca cgggggcccg ggggacagct ccgaggagga gtccagcccc
     7021 acctcccgct acatccactt ccctgtgact gtggtgtccg cccctggtct ggcccccagc
     7081 gctacccctg gagccccccg cattgaacag ctggacggcg tggacgacgg cactgacagt
     7141 gaggctgagg cggtgcagca gcctcggggc cagggcacgc ctccttcggg gccaggagta
     7201 gtccgggcag gggtccttgg ggctgcaggg gacagggccc ggcctcctga ggacctgcca
     7261 tcggaaattg tggattttgt gttgaagaac ctagggggtc ctggggatgg aggtgctggc
     7321 cctagagagg agtcactccc cccggcgcct cccctggcta atggcagcca gccctcccaa
     7381 ggcctgaccg ccagcccagc tgaccccacc cgcacatttg cctggctccc aggggcccca
     7441 ggggtccggg tgttaagcct tggccctgcc cctgagcccc ccaaacccgc cacatccaaa
     7501 atcatacttg tcaacaagct ggggcaagta tttgtgaaga tggctgggga gggtgaacct
     7561 gtcccacccc cagtgaagca gccacctttg ccccccacca tttcccccac ggctcccacc
     7621 tcctggactc tgcccccagg ccccctcctc ggcgtgctgc ccgtggtcgg agtggtccgc
     7681 cctgccccgc ccccgccacc ccctcccctg acgctggtgc tgagcagtgg gccagccagc
     7741 ccgccccgcc aggccatccg cgtcaagagg gtgtccactt tctccggccg gtccccgcca
     7801 gcacctcccc catacaaagc cccccggctg gatgaagatg gagaggcctc agaggatacc
     7861 cctcaggttc cagggcttgg cagtggcggg tttagccgtg tgaggatgaa aacccccaca
     7921 gtgcgtgggg tccttgacct ggatcggcct ggggagcccg ctggggaaga aagtcctggg
     7981 cccctccagg aacggtcccc tttgctgcca cttccggaag atggtcctcc ccaggtcccc
     8041 gatggtcccc cagacctgct gcttgagtcc cagtggcacc actattcagg tgaggcttcg
     8101 agctctgagg aagagcctcc atccccagat gataaagaga accaggcccc aaaacggact
     8161 ggcccacatc tgcgcttcga gatcagcagt gaggatgggt tcagcgttga ggcagagagc
     8221 ttggaggggg cgtggagaac tctgatcgag aaagtgcaag aggcccgagg gcatgcccga
     8281 ctcagacatc tctcctttag tggaatgagt ggggcgagac tcctgggcat ccaccatgat
     8341 gctgtcatct tcctggccga gcagctcccc ggagcccagc gttgccagca ctataagttc
     8401 cgttaccacc agcagggaga gggccaggag gagccgcccc tgaatcccca tggggctgct
     8461 cgggcagagg tctatctccg gaagtgcacc tttgacatgt tcaacttcct ggcctcccag
     8521 caccgggtgc tccctgaggg ggccacctgt gatgaggaag aggatgaggt gcagctcagg
     8581 tcaaccagac gtgccaccag cctggagctg cccatggcca tgcgttttcg tcaccttaag
     8641 aagacgtcca aagaagctgt gggtgtctac agatcagcca tccacgggcg aggcctgttc
     8701 tgtaagcgca acatcgacgc gggggagatg gtcatcgagt actctggcat tgtcatccgc
     8761 tcggtgttga ctgacaagcg ggagaagttc tacgatggga agggcatcgg gtgctatatg
     8821 ttccgcatgg atgactttga tgtagtggac gccacgatgc atggcaatgc cgcccgcttc
     8881 atcaaccact cctgtgagcc caactgcttc tctcgggtca tccacgtgga gggccagaaa
     8941 cacattgtta tcttcgccct gcgccgcatc ctgcgtggtg aggagctcac ctacgactac
     9001 aagttcccca tcgaggatgc cagcaacaag ctgccctgca actgtggcgc caagcgctgc
     9061 cgtcggttcc ttaacctcga gtacccatac gacgtacctg attatgcaac ttatccttat
     9121 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcatg agggcccgtt
     9181 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9241 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9301 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9361 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9421 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     9481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9901 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9961 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    10201 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    10261 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
    10321 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    10381 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    10441 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    10501 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    10561 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    10621 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    10681 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    10741 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    10801 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    10861 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    10921 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    10981 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    11041 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    11101 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    11161 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    11221 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    11281 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    11341 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11401 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    11461 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    11521 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    11581 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11641 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11701 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11761 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11821 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11881 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11941 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12001 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    12061 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    12121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    12181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    12241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    12301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    12361 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
    12421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    13021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    13081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    13141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    13201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
    13621 cccgatcccc tatggt
//
