LOCUS       V037875                 9007 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037875
VERSION     V037875
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4446
                     /label="LAMB3(NM_000228)"
                     /note="LAMB3(NM_000228)"
                     /gene="LAMB3"
     CDS             4453..4479
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4483..4509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4513..4539
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4574..4798
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4844..5272
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5286..5615
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5682..6476
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6650..6771
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6820..6836)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6844..6860
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6868..6898)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6913..6934
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7222..7807)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7978..8838)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8839..8943)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgagaccat tcttcctctt gtgttttgcc
      961 ctgcctggcc tcctgcatgc ccaacaagcc tgctcccgtg gggcctgcta tccacctgtt
     1021 ggggacctgc ttgttgggag gacccggttt ctccgagctt catctacctg tggactgacc
     1081 aagcctgaga cctactgcac ccagtatggc gagtggcaga tgaaatgctg caagtgtgac
     1141 tccaggcagc ctcacaacta ctacagtcac cgagtagaga atgtggcttc atcctccggc
     1201 cccatgcgct ggtggcagtc acagaatgat gtgaaccctg tctctctgca gctggacctg
     1261 gacaggagat tccagcttca agaagtcatg atggagttcc aggggcccat gcccgccggc
     1321 atgctgattg agcgctcctc agacttcggt aagacctggc gagtgtacca gtacctggct
     1381 gccgactgca cctccacctt ccctcgggtc cgccagggtc ggcctcagag ctggcaggat
     1441 gttcggtgcc agtccctgcc tcagaggcct aatgcacgcc taaatggggg gaaggtccaa
     1501 cttaacctta tggatttagt gtctgggatt ccagcaactc aaagtcaaaa aattcaagag
     1561 gtgggggaga tcacaaactt gagagtcaat ttcaccaggc tggcccctgt gccccaaagg
     1621 ggctaccacc ctcccagcgc ctactatgct gtgtcccagc tccgtctgca ggggagctgc
     1681 ttctgtcacg gccatgctga tcgctgcgca cccaagcctg gggcctctgc aggcccctcc
     1741 accgctgtgc aggtccacga tgtctgtgtc tgccagcaca acactgccgg cccaaattgt
     1801 gagcgctgtg cacccttcta caacaaccgg ccctggagac cggcggaggg ccaggacgcc
     1861 catgaatgcc aaaggtgcga ctgcaatggg cactcagaga catgtcactt tgaccccgct
     1921 gtgtttgccg ccagccaggg ggcatatgga ggtgtgtgtg acaattgccg ggaccacacc
     1981 gaaggcaaga actgtgagcg gtgtcagctg cactatttcc ggaaccggcg cccgggagct
     2041 tccattcagg agacctgcat ctcctgcgag tgtgatccgg atggggcagt gccaggggct
     2101 ccctgtgacc cagtgaccgg gcagtgtgtg tgcaaggagc atgtgcaggg agagcgctgt
     2161 gacctatgca agccgggctt cactggactc acctacgcca acccgcaggg ctgccaccgc
     2221 tgtgactgca acatcctggg gtcccggagg gacatgccgt gtgacgagga gagtgggcgc
     2281 tgcctttgtc tgcccaacgt ggtgggtccc aaatgtgacc agtgtgctcc ctaccactgg
     2341 aagctggcca gtggccaggg ctgtgaaccg tgtgcctgcg acccgcacaa ctccctcagc
     2401 ccacagtgca accagttcac agggcagtgc ccctgtcggg aaggctttgg tggcctgatg
     2461 tgcagcgctg cagccatccg ccagtgtcca gaccggacct atggagacgt ggccacagga
     2521 tgccgagcct gtgactgtga tttccgggga acagagggcc cgggctgcga caaggcatca
     2581 ggccgctgcc tctgccgccc tggcttgacc gggccccgct gtgaccagtg ccagcgaggc
     2641 tactgtaatc gctacccggt gtgcgtggcc tgccaccctt gcttccagac ctatgatgcg
     2701 gacctccggg agcaggccct gcgctttggt agactccgca atgccaccgc cagcctgtgg
     2761 tcagggcctg ggctggagga ccgtggcctg gcctcccgga tcctagatgc aaagagtaag
     2821 attgagcaga tccgagcagt tctcagcagc cccgcagtca cagagcagga ggtggctcag
     2881 gtggccagtg ccatcctctc cctcaggcga actctccagg gcctgcagct ggatctgccc
     2941 ctggaggagg agacgttgtc ccttccgaga gacctggaga gtcttgacag aagcttcaat
     3001 ggtctcctta ctatgtatca gaggaagagg gagcagtttg aaaaaataag cagtgctgat
     3061 ccttcaggag ccttccggat gctgagcaca gcctacgagc agtcagccca ggctgctcag
     3121 caggtctccg acagctcgcg ccttttggac cagctcaggg acagccggag agaggcagag
     3181 aggctggtgc ggcaggcggg aggaggagga ggcaccggca gccccaagct tgtggccctg
     3241 aggctggaga tgtcttcgtt gcctgacctg acacccacct tcaacaagct ctgtggcaac
     3301 tccaggcaga tggcttgcac cccaatatca tgccctggtg agctatgtcc ccaagacaat
     3361 ggcacagcct gtggctcccg ctgcaggggt gtccttccca gggccggtgg ggccttcttg
     3421 atggcggggc aggtggctga gcagctgcgg ggcttcaatg cccagctcca gcggaccagg
     3481 cagatgatta gggcagccga ggaatctgcc tcacagattc aatccagtgc ccagcgcttg
     3541 gagacccagg tgagcgccag ccgctcccag atggaggaag atgtcagacg cacacggctc
     3601 ctaatccagc aggtccggga cttcctaaca gaccccgaca ctgatgcagc cactatccag
     3661 gaggtcagcg aggccgtgct ggccctgtgg ctgcccacag actcagctac tgttctgcag
     3721 aagatgaatg agatccaggc cattgcagcc aggctcccca acgtggactt ggtgctgtcc
     3781 cagaccaagc aggacattgc gcgtgcccgc cggttgcagg ctgaggctga ggaagccagg
     3841 agccgagccc atgcagtgga gggccaggtg gaagatgtgg ttgggaacct gcggcagggg
     3901 acagtggcac tgcaggaagc tcaggacacc atgcaaggca ccagccgctc ccttcggctt
     3961 atccaggaca gggttgctga ggttcagcag gtactgcggc cagcagaaaa gctggtgaca
     4021 agcatgacca agcagctggg tgacttctgg acacggatgg aggagctccg ccaccaagcc
     4081 cggcagcagg gggcagaggc agtccaggcc cagcagcttg cggaaggtgc cagcgagcag
     4141 gcattgagtg cccaagaggg atttgagaga ataaaacaaa agtatgctga gttgaaggac
     4201 cggttgggtc agagttccat gctgggtgag cagggtgccc ggatccagag tgtgaagaca
     4261 gaggcagagg agctgtttgg ggagaccatg gagatgatgg acaggatgaa agacatggag
     4321 ttggagctgc tgcggggcag ccaggccatc atgctgcgct cagcggacct gacaggactg
     4381 gagaagcgtg tggagcagat ccgtgaccac atcaatgggc gcgtgctcta ctatgccacc
     4441 tgcaagctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct
     4501 gattatgcta gctatcctta tgatgtccca gactacgcat gagggcccgt ttaaacccgc
     4561 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4681 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4741 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4801 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5281 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5341 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5461 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5521 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5581 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5641 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5701 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5761 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5821 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5881 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5941 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6001 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6061 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6121 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6181 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6241 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6301 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6361 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6421 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6481 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6541 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6601 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6781 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6841 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6901 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6961 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7741 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8941 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     9001 ctatggt
//
