LOCUS V038267 6862 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038267 VERSION V038267 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 874..1590 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1642..2352 /label="LYPLAL1(NM_138794)" /note="LYPLAL1(NM_138794)" /gene="LYPLAL1" polyA_signal 2429..2653 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2699..3127 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3141..3470 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3537..4331 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4505..4626 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4675..4691) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4699..4715 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4723..4753) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4768..4789 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5077..5662) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5833..6693) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6694..6798) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc 1621 ggaggcggag ggagtgaatt catggcggct gcgtcggggt cggttctgca gcgctgtatc 1681 gtgtcgccgg cagggaggca tagcgcctct ctgatcttcc tgcatggctc aggtgattct 1741 ggacaaggat taagaatgtg gatcaagcag gttttaaatc aagatttaac attccaacac 1801 ataaaaatta tttatccaac agctcctccc agatcatata ctcctatgaa aggaggaatc 1861 tccaatgtat ggtttgacag atttaaaata accaatgact gcccagaaca ccttgaatca 1921 attgatgtca tgtgtcaagt gcttactgat ttgattgatg aagaagtaaa aagtggcatc 1981 aagaagaaca ggatattaat aggaggattc tctatgggag gatgcatggc aatacattta 2041 gcatatagaa atcatcaaga tgtggcagga gtatttgctc tttctagttt tctgaataaa 2101 gcatctgctg tttaccaggc tcttcagaag agtaatggtg tacttcctga attatttcag 2161 tgtcatggta ctgcagatga gttagttctt cattcttggg cagaagagac aaactcaatg 2221 ttaaaatctc taggagtgac cacgaagttt catagttttc caaatgttta ccatgagcta 2281 agcaaaactg agttagacat attgaagtta tggattctta caaagctgcc aggagaaatg 2341 gaaaaacaaa aatgaggatc ctgatatcca gcacagtggc ggccgctcga gtctagaggg 2401 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 2461 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 2521 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 2581 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 2641 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 3061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 3481 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 3541 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 3601 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 3661 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 3721 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3781 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3841 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 3901 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 3961 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 4021 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 4081 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 4141 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 4201 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 4261 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 4321 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 4381 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4441 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4501 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4621 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 4681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 4741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 4801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 4921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 5041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 5101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 5161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 5221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 5281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 5341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 5401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 5461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 5521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 5581 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac 5641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 5701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 5761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 5821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 5881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 5941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 6001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 6061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 6121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 6181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 6241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 6301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 6361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 6421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 6481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 6541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 6601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 6661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 6721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 6781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 6841 agatctcccg atcccctatg gt //