LOCUS V039486 6758 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039486 VERSION V039486 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..2230 /label="MAPT(NM_005910)" /note="MAPT(NM_005910)" /gene="MAPT" CDS 2231..2251 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /transl_table=1 /translation="ENLYFQG" /label="TEV site" CDS 2261..2278 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 2325..2549 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2595..3023 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3037..3366 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3433..4227 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4401..4522 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4571..4587) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4595..4611 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4619..4649) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4664..4685 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4973..5558) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5729..6589) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6590..6694) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gctgagcccc gccaggagtt cgaagtgatg gaagatcacg ctgggacgta 961 cgggttgggg gacaggaaag atcagggggg ctacaccatg caccaagacc aagagggtga 1021 cacggacgct ggcctgaaag aatctcccct gcagaccccc actgaggacg gatctgagga 1081 accgggctct gaaacctctg atgctaagag cactccaaca gcggaagatg tgacagcacc 1141 cttagtggat gagggagctc ccggcaagca ggctgccgcg cagccccaca cggagatccc 1201 agaaggaacc acagctgaag aagcaggcat tggagacacc cccagcctgg aagacgaagc 1261 tgctggtcac gtgacccaag ctcgcatggt cagtaaaagc aaagacggga ctggaagcga 1321 tgacaaaaaa gccaaggggg ctgatggtaa aacgaagatc gccacaccgc ggggagcagc 1381 ccctccaggc cagaagggcc aggccaacgc caccaggatt ccagcaaaaa ccccgcccgc 1441 tccaaagaca ccacccagct ctggtgaacc tccaaaatca ggggatcgca gcggctacag 1501 cagccccggc tccccaggca ctcccggcag ccgctcccgc accccgtccc ttccaacccc 1561 acccacccgg gagcccaaga aggtggcagt ggtccgtact ccacccaagt cgccgtcttc 1621 cgccaagagc cgcctgcaga cagcccccgt gcccatgcca gacctgaaga atgtcaagtc 1681 caagatcggc tccactgaga acctgaagca ccagccggga ggcgggaagg tgcagataat 1741 taataagaag ctggatctta gcaacgtcca gtccaagtgt ggctcaaagg ataatatcaa 1801 acacgtcccg ggaggcggca gtgtgcaaat agtctacaaa ccagttgacc tgagcaaggt 1861 gacctccaag tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga 1921 agtaaaatct gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga 1981 caatatcacc cacgtccctg gcggaggaaa taaaaagatt gaaacccaca agctgacctt 2041 ccgcgagaac gccaaagcca agacagacca cggggcggag atcgtgtaca agtcgccagt 2101 ggtgtctggg gacacgtctc cacggcatct cagcaatgtc tcctccaccg gcagcatcga 2161 catggtagac tcgccccagc tcgccacgct agctgacgag gtgtctgcct ccctggccaa 2221 gcagggtttg gagaatctct acttccaggg agcggccgca catcaccatc atcaccattg 2281 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 2341 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 2401 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 2461 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 2521 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 2581 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 2641 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 2701 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 2761 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 2821 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 2881 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 2941 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3001 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 3061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 3241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 3301 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 3361 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 3421 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 3481 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 3541 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 3601 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 3661 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 3721 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 3781 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 3841 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 3901 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 3961 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4021 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4081 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4141 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4201 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 4261 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4321 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4381 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 4441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4501 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 4561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 5521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 6001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 6061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 6121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 6241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 6301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 6361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6721 acgtcgacgg atcgggagat ctcccgatcc cctatggt //