LOCUS       V026547                 9008 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026547
VERSION     V026547
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..4498
                     /label="MYT1L(NM_001303052)"
                     /note="MYT1L(NM_001303052)"
                     /gene="MYT1L"
     CDS             4505..4570
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4608..4832
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4878..5306
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5320..5649
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5716..6510
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6684..6805
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6854..6870)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6878..6894
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6902..6932)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6947..6968
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7256..7841)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8012..8872)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8873..8977)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggaggtgg acaccgagga
      961 gaagcggcat cgcacgcggt ccaaaggggt tcgagttccc gtggaaccag ccatacaaga
     1021 gctgttcagc tgtcccaccc ctggctgtga cggcagtggt catgtcagtg gcaaatatgc
     1081 aagacacaga agtgtatatg gttgtccctt ggcgaaaaaa agaaaaacac aagataaaca
     1141 gccccaggaa cctgctccta aacgaaagcc atttgccgtg aaagcagaca gctcctcagt
     1201 ggatgagtgt gacgacagtg atgggactga ggacatggat gagaaggagg aggatgaggg
     1261 ggaggagtac tccgaggaca atgatgagcc aggggatgag gacgaggagg acgaggaggg
     1321 ggaccgggag gaggaggagg agatcgagga ggaggatgag gacgatgacg aggatggaga
     1381 agatgtggag gatgaagaag aggaagagga ggaggaggag gaggaggaag aggaagaaga
     1441 aaacgaagac catcaaatga attgtcacaa tactcgaata atgcaagaca cagaaaagga
     1501 tgataacaat aatgacgaat atgacaatta cgatgaactg gtggccaagt cattgttaaa
     1561 cctcggcaaa atcgctgagg atgcagccta ccgggccagg actgagtcag aaatgaacag
     1621 caatacctcc aatagtctgg aagacgatag tgacaaaaac gaaaacctgg gtcggaaaag
     1681 tgagttgagt ttagacttag acagtgatgt tgttagagaa acagtggact cccttaaact
     1741 attagcccaa ggacacggtg ttgtgctctc agaaaacatg aatgacagaa attatgcaga
     1801 cagcatgtcg cagcaagaca gtagaaatat gaattacgtc atgttgggga agcccatgaa
     1861 caacggactc atggaaaaga tggtggagga gagcgatgag gaggtgtgtc tgagcagtct
     1921 ggagtgtttg aggaatcagt gcttcgacct ggccaggaag ctcagtgaga ccaacccgca
     1981 ggagaggaat ccgcagcaga acatgaacat ccgtcagcat gtccggccag aagaggactt
     2041 ccccggaagg acgccggaca gaaactactc ggacatgctg aacctcatgc ggctggagga
     2101 gcagttgagc ccccggtcga gagtgtttgc cagctgtgcg aaggaggatg ggtgtcatga
     2161 gcgggacgac gataccacct ctgtgaactc ggacaggtct gaagaggtgt tcgacatgac
     2221 caaggggaac ctgaccctgc tggagaaagc catcgctttg gaaacggaaa gagcaaaggc
     2281 catgagggag aagatggcca tggaagctgg gaggagggac aatatgaggt catatgagga
     2341 ccagtctccg agacaacttc ccggggagga cagaaagcct aaatccagtg acagccatgt
     2401 caaaaagcca tactatggta aagatccctc aagaacagaa aagaaagaga gcaagtgtcc
     2461 aacccccggg tgtgatggaa ccggccacgt aactgggctg tacccacatc accgcagcct
     2521 gtccggatgc ccgcacaaag atagggtccc tccagaaatc cttgccatgc atgaaagtgt
     2581 cctcaagtgc cccactccgg gctgcacggg gcgcgggcat gtcaacagca acaggaactc
     2641 ccaccgaagc ctctccggat gcccgatcgc tgcagcagag aaactggcca aggcacagga
     2701 aaagcaccag agctgcgacg tgtccaagtc cagccaggcc tcggaccgcg tgctcaggcc
     2761 aatgtgcttt gtgaagcagc tggagattcc tcagtatggc tacagaaaca atgtccccac
     2821 aactacgccg cgttccaacc tggccaagga gctcgagaaa tattccaaga cctcgtttga
     2881 atacaacagt tacgacaacc atacttatgg caagcgagcc atagctccca aggtgcaaac
     2941 cagggatata tcccccaaag gatatgatga tgcgaagcgg tactgcaagg accccagccc
     3001 cagcagcagc agcaccagca gctacgcgcc cagcagcagc agcaacctga gctgcggcgg
     3061 gggcagcagc gccagcagca cgtgcagcaa gagcagcttc gactacacgc acgacatgga
     3121 ggcggcccac atggcggcca ccgccatcct caacctgtcc acgcgctgcc gcgagatgcc
     3181 gcagaacctg agcaccaagc cgcaggacct gtgcgccacg cggaaccctg acatggaggt
     3241 ggatgagaac gggaccctgg acctcagcat gaacaagcag aggccgcggg acagctgctg
     3301 ccccatcctg acccctctgg agcccatgtc cccccagcag caggcagtga tgaacaaccg
     3361 gtgtttccag ctgggcgagg gcgactgctg ggacttgccc gtagactaca ccaaaatgaa
     3421 accccggagg atagacgagg acgagtccaa agacattact ccagaagact tggacccatt
     3481 ccaggaggct ctagaagaaa gacggtatcc cggggaggtg accatcccaa gtcccaaacc
     3541 caagtaccct cagtgcaagg agagcaaaaa ggacttaata actctgtctg gctgccccct
     3601 ggcggacaaa agcattcgaa gtatgctggc caccagctcc caagaactca agtgccccac
     3661 gcctggctgt gatggttctg gacatatcac cggcaattat gcttctcatc ggagcctttc
     3721 aggttgccca agagcaaaga aaagtggtat caggatagca cagagcaaag aagataaaga
     3781 agatcaagaa cccatcaggt gtccggtccc cgggtgcgac ggccagggcc acatcactgg
     3841 gaagtacgcg tcccatcgca gcgcctccgg gtgccccttg gcggccaaga ggcagaaaga
     3901 cgggtacctg aatggctccc agttctcctg gaagtcggtc aagacggaag gcatgtcctg
     3961 ccccacgcca ggatgcgacg gctcaggcca cgtcagcggc agcttcctca cacaccgcag
     4021 cttgtcagga tgcccgagag ccacgtcagc gatgaagaag gcaaagcttt ctggagagca
     4081 gatgctgacc atcaaacagc gggccagcaa cggtatagaa aatgatgaag aaatcaaaca
     4141 gttagatgaa gaaatcaagg agctaaatga atccaattcc cagatggaag ccgatatgat
     4201 taaactcaga actcagatta ccacgatgga gagcaacctg aagaccatcg aagaggagaa
     4261 caaagtgatt gagcagcaga acgagtctct cctccacgag ctggcgaacc tgagccagtc
     4321 tctgatccac agcctggcta acatccagct gccgcacatg gatccaatca atgaacaaaa
     4381 ttttgatgct tacgtgacta ctttgacgga aatgtataca aatcaagatc gttatcagag
     4441 tccagaaaat aaagccctac tggaaaatat aaagcaggct gtgagaggaa ttcaggtcct
     4501 cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     4561 cgatgacaag tagtgagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4621 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4681 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4741 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4801 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4861 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5281 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5401 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5461 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5581 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5641 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     5701 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5761 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5821 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5881 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5941 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6001 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6061 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6121 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6181 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6241 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6301 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6361 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6421 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6481 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6541 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6601 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6661 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6781 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6841 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9001 ctgacgtc
//
