LOCUS V045944 7008 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045944 VERSION V045944 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 887..1768 /label="NPM1(NM_002520)" /note="NPM1(NM_002520)" /gene="NPM1" CDS 1814..2524 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 2575..2799 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2845..3273 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3287..3616 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3683..4477 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4651..4772 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4821..4837) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4845..4861 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4869..4899) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4914..4935 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5223..5808) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5979..6839) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6840..6944) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcaac ttaaggatcc gccaccatgg aagattcgat 901 ggacatggac atgagccccc tgaggcccca gaactatctt ttcggttgtg aactaaaggc 961 cgacaaagat tatcacttta aggtggataa tgatgaaaat gagcaccagt tatctttaag 1021 aacggtcagt ttaggggctg gtgcaaagga tgagttgcac attgttgaag cagaggcaat 1081 gaattacgaa ggcagtccaa ttaaagtaac actggcaact ttgaaaatgt ctgtacagcc 1141 aacggtttcc cttgggggct ttgaaataac accaccagtg gtcttaaggt tgaagtgtgg 1201 ttcagggcca gtgcatatta gtggacagca cttagtagct gtggaggaag atgcagagtc 1261 agaagatgaa gaggaggagg atgtgaaact cttaagtata tctggaaagc ggtctgcccc 1321 tggaggtggt agcaaggttc cacagaaaaa agtaaaactt gctgctgatg aagatgatga 1381 cgatgatgat gaagaggatg atgatgaaga tgatgatgat gatgattttg atgatgagga 1441 agctgaagaa aaagcgccag tgaagaaatc tatacgagat actccagcca aaaatgcaca 1501 aaagtcaaat cagaatggaa aagactcaaa accatcatca acaccaagat caaaaggaca 1561 agaatccttc aagaaacagg aaaaaactcc taaaacacca aaaggaccta gttctgtaga 1621 agacattaaa gcaaaaatgc aagcaagtat agaaaaaggt ggttctcttc ccaaagtgga 1681 agccaaattc atcaattatg tgaagaattg cttccggatg actgaccaag aggctattca 1741 agatctctgg cagtggagga agtctcttgg aggcggaggg agtggtggag gcggatcagg 1801 aggcggtgga tctatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 1861 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 1921 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 1981 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 2041 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 2101 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 2161 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 2221 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 2281 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 2341 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 2401 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 2461 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 2521 gtaggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc 2581 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 2641 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 2701 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 2761 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 2821 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 2881 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2941 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 3001 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 3061 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 3121 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 3181 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 3241 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3361 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3421 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 3481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 3541 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 3601 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 3661 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 3721 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 3781 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 3841 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 3901 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 3961 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 4021 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 4081 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 4141 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 4201 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 4261 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 4321 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 4381 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 4441 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 4501 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 4561 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 4621 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 4681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4741 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 4801 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 4861 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 4921 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4981 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5641 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 5701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 6961 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggt //