LOCUS       V042942                 8642 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042942
VERSION     V042942
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..2335
                     /label="NR1H4(NM_001206979)"
                     /note="NR1H4(NM_001206979)"
                     /gene="NR1H4"
     CDS             2336..2392
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3422..3475
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             3476..3703
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /transl_table=1
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
                     /label="ubiquitin"
     CDS             3704..3931
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /transl_table=1
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
                     /label="ubiquitin"
     CDS             3932..4159
                     /codon_start=1
                     /product="human ubiquitin"
                     /transl_table=1
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
                     /label="ubiquitin"
     polyA_signal    4209..4433
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4479..4907
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4921..5250
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5317..6111
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6285..6406
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6455..6471)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6479..6495
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6503..6533)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6548..6569
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6857..7442)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7613..8473)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8474..8578)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg ggatcaaaaa tgaatctcat tgaacattcc catttaccta ccacagatga
      961 attttctttt tctgaaaatt tatttggtgt tttaacagaa caagtggcag gtcctctggg
     1021 acagaacctg gaagtggaac catactcgca atacagcaat gttcagtttc cccaagttca
     1081 accacagatt tcctcgtcat cctattattc caacctgggt ttctaccccc agcagcctga
     1141 agagtggtac tctcctggaa tatatgaact caggcgtatg ccagctgaga ctctctacca
     1201 gggagaaact gaggtagcag agatgcctgt aacaaagaag ccccgcatgg gcgcgtcagc
     1261 agggaggatc aaaggggatg agctgtgtgt tgtttgtgga gacagagcct ctggatacca
     1321 ctataatgca ctgacctgtg aggggtgtaa aggtttcttc aggagaagca ttaccaaaaa
     1381 cgctgtgtac aagtgtaaaa acgggggcaa ctgtgtgatg gatatgtaca tgcgaagaaa
     1441 gtgtcaagag tgtcgactaa ggaaatgcaa agagatggga atgttggctg aatgtatgta
     1501 tacaggcttg ttaactgaaa ttcagtgtaa atctaagcga ctgagaaaaa atgtgaagca
     1561 gcatgcagat cagaccgtga atgaagacag tgaaggtcgt gacttgcgac aagtgacctc
     1621 gacaacaaag tcatgcaggg agaaaactga actcacccca gatcaacaga ctcttctaca
     1681 ttttattatg gattcatata acaaacagag gatgcctcag gaaataacaa ataaaatttt
     1741 aaaagaagaa ttcagtgcag aagaaaattt tctcattttg acggaaatgg caaccaatca
     1801 tgtacaggtt cttgtagaat tcacaaaaaa gctaccagga tttcagactt tggaccatga
     1861 agaccagatt gctttgctga aagggtctgc ggttgaagct atgttccttc gttcagctga
     1921 gattttcaat aagaaacttc cgtctgggca ttctgaccta ttggaagaaa gaattcgaaa
     1981 tagtggtatc tctgatgaat atataacacc tatgtttagt ttttataaaa gtattgggga
     2041 actgaaaatg actcaagagg agtatgctct gcttacagca attgttatcc tgtctccaga
     2101 tagacaatac ataaaggata gagaggcagt agagaagctt caggagccac ttcttgatgt
     2161 gctacaaaag ttgtgtaaga ttcaccagcc tgaaaatcct caacactttg cctgtctcct
     2221 gggtcgcctg actgaattac ggacattcaa tcatcaccac gctgagatgc tgatgtcatg
     2281 gagagtaaac gaccacaagt ttaccccact tctctgtgaa atctgggacg tgcaggcaac
     2341 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggcaga
     2401 gcagctttct ccaggaaagg cggtggatca ggtgtgcacc ttccttttca aaaagcctgg
     2461 gcggaaaggg gctgctggac gcagaaagcg cccggcctgc gacccagagc ccggagaaag
     2521 cggcagcagt agcgacgaag gctgcactgt ggttcgaccg gaaaagaagc gggtgaccca
     2581 caatccaatg atacagaaga cccgtgacag tggtaaacag aaggcggctt acggcgactt
     2641 gagcagcgaa gaggaagagg aaaatgagcc cgagagtctc ggcgtggttt ataaatccac
     2701 ccgttcggcg aaacccgtgg gaccagagga tatgggagcg acagctgtct atgagctgga
     2761 cacagagaaa gagcgcgatg cacaagccat ctttgagcgc agccagaaga tccaggagga
     2821 gctgaggggc aaggaggatg acaagatcta tcggggaatc aacaattatc agaaatacat
     2881 gaagcccaag gatacgtcta tgggcaatgc ctcttccggg atggtgagga agggccccat
     2941 ccgagcgccc gagcatctac gtgccaccgt gcgctgggat taccagcccg acatctgtaa
     3001 ggactacaaa gagactggct tctgcggctt cggagacagc tgcaaattcc tccatgaccg
     3061 ttcagattac aagcatgggt ggcagatcga acgtgagctt gatgagggtc gctatggtgt
     3121 ctatgaggat gaaaactatg aagtgggaag cgatgatgag gaaataccat tcaagtgttt
     3181 catctgtcgc cagagcttcc aaaacccagt tgtcaccaag tgcaggcatt atttctgcga
     3241 gagctgtgca ctgcagcatt tccgcaccac cccgcgctgc tatgtctgtg accagcagac
     3301 caatggcgtc ttcaatccag cgaaagaatt gattgctaaa ctagagaagc atcgagctac
     3361 aggagagggt ggtgcttccg acttgccaga agaccccgat gaggatgcaa ttcccattac
     3421 tgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgca
     3481 gatcttcgtg aaaaccctta ccggcaagac catcaccctt gaggtggagc ccagtgacac
     3541 catcgaaaat gtgaaggcca agatccagga taaggaaggc attccccccg accagcagag
     3601 gctcatcttt gcaggcaagc agctggaaga tggccgtact ctttctgact acaacatcca
     3661 gaaggagtcg accctgcacc tggtcctgcg tctgagaggt ggtatgcaga tcttcgtgaa
     3721 gaccctgacc ggcaagacca tcaccctgga agtggagccc agtgacacca tcgaaaatgt
     3781 gaaggccaag atccaggata aagaaggcat ccctcccgac cagcagaggc tcatctttgc
     3841 aggcaagcag ctggaagatg gccgcactct ttctgactac aacatccaga aggagtcgac
     3901 cctgcacctg gtcctgcgtc tgagaggtgg tatgcagatc ttcgtgaaga ccctgaccgg
     3961 caagaccatc actctggagg tggagcccag tgacaccatc gaaaatgtga aggccaagat
     4021 ccaagataaa gaaggcatcc cccccgacca gcagaggctc atctttgcag gcaagcagct
     4081 ggaagatggc cgcactcttt ctgactacaa catccagaaa gagtcgaccc tgcacctggt
     4141 cctgcgcctg aggggtggct gttaactcga gtctagaggg cccgtttaaa cccgctgatc
     4201 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4261 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4321 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4381 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4441 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4501 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4561 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4621 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4681 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4741 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4801 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4861 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4921 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4981 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5041 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5101 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5161 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5221 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5281 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5341 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5401 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5461 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5521 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5581 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5641 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5701 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5761 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5821 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5881 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5941 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6001 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6061 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6121 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7381 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8581 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8641 gt
//
